MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy1
MS data file : PRT1311_JBEI_1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 8 Sep 2025 at 17:17:19 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,450

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 401–500 (out of 4217)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
161 306.1562 305.1489
162 306.1562 305.1489
163 307.0103 306.0030
164 307.0104 306.0031
165 307.0777 306.0704
166 307.0778 306.0705
167 307.0779 306.0706
168 307.0783 306.0710
169 307.0789 306.0716
170 307.1136 306.1063
171 307.1151 306.1078
172 307.1163 306.1090
173 307.1166 306.1093
174 307.1167 306.1094
175 307.1167 306.1094
176 307.1167 306.1094
177 307.1167 306.1094
178 307.1167 306.1094
179 307.1168 306.1095
180 307.1168 306.1095
181 307.1168 306.1095
182 307.1169 306.1096
183 307.1169 306.1096
184 307.1169 306.1096
185 307.1169 306.1096
186 307.1533 306.1460
187 307.1534 306.1461
188 307.1534 306.1461
189 307.1535 306.1462
190 307.1535 306.1462
191 307.1535 306.1462
192 307.1535 306.1462
193 307.1535 306.1462
194 307.1535 306.1462
195 307.1536 306.1463
196 307.1536 306.1463
197 307.1537 306.1464
198 307.1537 306.1464
199 307.1537 306.1464
200 307.1537 306.1464
201 307.1538 306.1465
202 307.1539 306.1466
203 307.1550 306.1477
204 307.1905 306.1832
205 307.1906 306.1833
206 307.1906 306.1833
207 307.1906 306.1833
208 307.1907 306.1834
209 307.1907 306.1834
210 307.1908 306.1835
211 307.1908 306.1835
212 307.1908 306.1835
213 307.1908 306.1835
214 307.1909 306.1836
215 307.1910 306.1837
216 307.1910 306.1837
217 307.1910 306.1837
218 307.1910 306.1837
219 307.1913 306.1840
220 307.1915 306.1842
221 307.1916 306.1843
222 307.2101 306.2028
223 307.2107 306.2034
224 307.2114 306.2041
225 307.9938 306.9865
226 309.0931 308.0858
227 309.0934 308.0861
228 309.0942 308.0869
229 309.0947 308.0874
230 309.0947 308.0874
231 309.0951 308.0878
232 309.0952 308.0879
233 309.1318 308.1245
234 309.1322 308.1249
235 309.1324 308.1251
236 309.1325 308.1252
237 309.1325 308.1252
238 309.1326 308.1253
239 309.1326 308.1253
240 309.1326 308.1253
241 309.1327 308.1254
242 309.1327 308.1254
243 309.1328 308.1255
244 309.1328 308.1255
245 309.1329 308.1256
246 309.1331 308.1258
247 309.1331 308.1258
248 309.1333 308.1260
249 309.1333 308.1260
250 309.1334 308.1261
251 309.1335 308.1262
252 309.1693 308.1620
253 309.1694 308.1621
254 309.1694 308.1621
255 309.1694 308.1621
256 309.1695 308.1622
257 309.1695 308.1622
258 309.1695 308.1622
259 309.1695 308.1622
260 309.1695 308.1622
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