MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy1
MS data file : PRT1311_JBEI_1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 8 Sep 2025 at 17:17:19 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,450

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 4101–4200 (out of 4217)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4330 1144.2574 3429.7504
4331 1144.5901 3430.7485
4332 1144.5903 3430.7491
4333 1144.6018 3430.7836
4334 863.6760 3450.6749
4335 1160.2751 3477.8035
4336 1160.2756 3477.8050
4337 870.4604 3477.8125
4338 1160.6096 3478.8070
4339 870.7105 3478.8129
4340 879.6537 3514.5857
4341 1172.5372 3514.5898
4342 881.6633 3522.6241
4343 881.6641 3522.6273
4344 881.9122 3523.6197
4345 881.9128 3523.6221
4346 881.9128 3523.6221
4347 881.9134 3523.6245
4348 881.9136 3523.6253
4349 882.1629 3524.6225
4350 882.1642 3524.6277
4351 882.1680 3524.6429
4352 705.9409 3524.6681
4353 882.1744 3524.6685
4354 706.1418 3525.6726
4355 706.3403 3526.6651
4356 889.4170 3553.6389
4357 906.9907 3623.9337
4358 918.2168 3668.8381
4360 736.2022 3675.9746
4361 748.4095 3737.0111
4362 946.4861 3781.9153
4363 946.4874 3781.9205
4364 946.4890 3781.9269
4365 946.7366 3782.9173
4366 950.4903 3797.9321
4367 953.2375 3808.9209
4368 958.2786 3829.0853
4369 808.8195 4039.0611
4370 809.0206 4040.0666
4371 1013.0200 4048.0509
4373 1013.0208 4048.0541
4374 1013.0209 4048.0545
4375 1013.0216 4048.0573
4376 1013.0223 4048.0601
4377 1013.0233 4048.0641
4378 1013.0240 4048.0669
4379 1013.0255 4048.0729
4380 1016.5266 4062.0773
4381 1016.5291 4062.0873
4382 1016.7748 4063.0701
4383 1051.5544 4202.1885
4384 1063.7699 4251.0505
4385 1083.5719 4330.2585
4386 1087.5635 4346.2249
4387 1087.5786 4346.2853
4388 874.6417 4368.1721
4389 1123.8397 4491.3297
4390 1128.8816 4511.4973
4391 903.3079 4511.5031
4392 937.9084 4684.5056
4394 1172.3779 4685.4825
4395 1172.3792 4685.4877
4396 1172.3809 4685.4945
4397 938.5125 4687.5261
4398 1177.8776 4707.4813
4399 1178.8813 4711.4961
4400 1179.6377 4714.5217
4401 945.6888 4723.4076
4402 945.8976 4724.4516
4403 956.2055 4775.9911
4404 987.5366 4932.6466
4405 849.8217 5092.8865
4406 646.6415 5165.0738
4407 1112.5986 5557.9566
4408 927.6558 5559.8911
4409 927.6565 5559.8953
4410 930.3227 5575.8925
4411 1118.7467 5588.6971
4412 1125.3790 5621.8586
4413 703.9125 5623.2418
4414 1164.5905 5817.9161
4415 1167.5853 5832.8901
4416 1170.3927 5846.9271
4417 1005.5095 6027.0133
4418 1005.6752 6028.0075
4419 1005.6759 6028.0117
4420 1010.1741 6055.0009
4421 1011.1679 6060.9637
4422 1015.0101 6084.0169
4423 770.3169 6154.4770
4424 684.8389 6154.4846
4425 880.2223 6154.5052
4426 901.3198 6302.1877
4427 914.7682 6396.3265
4428 800.9258 6399.3482
4429 1075.4126 6446.4319
4430 922.0638 6447.3957
4431 922.2136 6448.4443
4432 1078.0699 6462.3757
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