MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy1
MS data file : PRT1311_JBEI_1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 8 Sep 2025 at 17:17:19 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,450

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 4001–4100 (out of 4217)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4212 922.1320 2763.3742
4214 922.7965 2765.3677
4216 926.1685 2775.4837
4217 927.4915 2779.4527
4218 927.9883 2780.9431
4219 930.4946 2788.4620
4220 932.1590 2793.4552
4221 935.1814 2802.5224
4222 937.1584 2808.4534
4223 937.3264 2808.9574
4224 937.3292 2808.9658
4225 937.4884 2809.4434
4227 942.1218 2823.3436
4228 942.4501 2824.3285
4229 950.8672 2849.5798
4230 950.8699 2849.5879
4231 950.8706 2849.5900
4232 950.8710 2849.5912
4233 955.4727 2863.3963
4234 718.3750 2869.4709
4235 718.3769 2869.4785
4236 963.8069 2888.3989
4237 965.1873 2892.5401
4238 975.2011 2922.5815
4239 975.2043 2922.5911
4240 975.5394 2923.5964
4241 978.5155 2932.5247
4242 982.8124 2945.4154
4244 1003.7258 3008.1556
4245 1006.2411 3015.7015
4246 1008.1699 3021.4879
4247 1008.5120 3022.5142
4248 1008.5148 3022.5226
4249 1008.8484 3023.5234
4250 1008.8496 3023.5270
4251 1008.8497 3023.5273
4253 1008.8519 3023.5339
4255 1010.1750 3027.5032
4256 1014.7889 3041.3449
4257 1014.8444 3041.5114
4258 764.1683 3052.6441
4260 1018.8924 3053.6554
4261 1024.6888 3071.0446
4262 1024.8549 3071.5429
4263 1025.8915 3074.6527
4264 1025.9305 3074.7697
4265 1026.2135 3075.6187
4266 1026.3701 3076.0885
4267 1034.5955 3100.7647
4268 776.2017 3100.7777
4269 1034.9255 3101.7547
4270 1042.2552 3123.7438
4271 1048.3688 3142.0846
4272 1051.3627 3151.0663
4273 1072.5317 3214.5733
4274 1072.5358 3214.5856
4275 649.7399 3243.6631
4277 814.1904 3252.7325
4278 1089.5350 3265.5832
4279 1089.8646 3266.5720
4280 818.4164 3269.6365
4289 1093.9436 3278.8090
4292 820.9545 3279.7889
4293 1101.2606 3300.7600
4294 1101.5958 3301.7656
4295 1101.5973 3301.7701
4296 826.6938 3302.7461
4297 826.6960 3302.7549
4298 1103.2616 3306.7630
4299 1103.2656 3306.7750
4300 827.9496 3307.7693
4301 1103.5996 3307.7770
4302 1103.6003 3307.7791
4303 827.9525 3307.7809
4304 1103.6034 3307.7884
4305 830.1886 3316.7253
4306 830.1890 3316.7269
4307 830.4394 3317.7285
4308 1106.9210 3317.7412
4309 830.4466 3317.7573
4310 831.6912 3322.7357
4311 1108.9214 3323.7424
4312 1110.5881 3328.7425
4313 1110.6042 3328.7908
4314 834.1808 3332.6941
4315 834.1848 3332.7101
4316 1112.5872 3334.7398
4317 1112.9287 3335.7643
4318 1113.2739 3336.7999
4319 835.4524 3337.7805
4320 1116.1857 3345.5353
4321 837.4370 3345.7189
4322 837.4407 3345.7337
4323 1117.2400 3348.6982
4324 675.1401 3370.6641
4325 1132.2108 3393.6106
4326 850.2023 3396.7801
4327 850.2025 3396.7809
4328 850.2031 3396.7833
4329 850.4547 3397.7897
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