MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy1
MS data file : PRT1311_JBEI_1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 8 Sep 2025 at 17:17:19 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,450

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3901–4000 (out of 4217)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4074 790.4240 2368.2502
4075 790.7565 2369.2477
4076 790.7597 2369.2573
4077 790.7640 2369.2702
4080 793.0809 2376.2209
4082 595.5608 2378.2141
4083 595.8076 2379.2013
4084 476.8481 2379.2041
4085 595.8097 2379.2097
4086 596.0562 2380.1957
4087 596.0564 2380.1965
4088 797.4268 2389.2586
4089 797.4305 2389.2697
4090 798.3713 2392.0921
4091 1197.0896 2392.1646
4092 799.4065 2395.1977
4094 799.4083 2395.2031
4095 799.7446 2396.2120
4096 800.1044 2397.2914
4098 800.4344 2398.2814
4100 805.1240 2412.3502
4101 805.4127 2413.2163
4103 811.4232 2431.2478
4104 811.4244 2431.2514
4106 811.4247 2431.2523
4107 811.7574 2432.2504
4108 813.7860 2438.3362
4109 816.7325 2447.1757
4110 816.7666 2447.2780
4111 816.7673 2447.2801
4112 816.7678 2447.2816
4113 817.1018 2448.2836
4114 818.4164 2452.2274
4115 818.7355 2453.1847
4117 818.7484 2453.2234
4118 818.7510 2453.2312
4119 819.0859 2454.2359
4120 820.4200 2458.2382
4121 820.7557 2459.2453
4125 824.0735 2469.1987
4126 618.3184 2469.2445
4127 618.3185 2469.2449
4129 824.0897 2469.2473
4130 824.4071 2470.1995
4131 311.9458 2487.5082
4132 833.7414 2498.2024
4134 626.3215 2501.2569
4135 627.5635 2506.2249
4136 628.0823 2508.3001
4137 841.0696 2520.1870
4138 841.4362 2521.2868
4139 843.4063 2527.1971
4141 846.0573 2535.1501
4143 846.0596 2535.1570
4144 848.4004 2542.1794
4149 849.0723 2544.1951
4154 853.3893 2557.1461
4155 854.0690 2559.1852
4156 854.4037 2560.1893
4158 854.4055 2560.1947
4159 856.7258 2567.1556
4160 856.7269 2567.1589
4162 643.0465 2568.1569
4163 643.0468 2568.1581
4166 858.4062 2572.1968
4167 858.7395 2573.1967
4168 859.0748 2574.2026
4169 859.4229 2575.2469
4170 860.3941 2578.1605
4171 860.7258 2579.1556
4172 646.2925 2581.1409
4173 861.4476 2581.3210
4174 861.7202 2582.1388
4175 517.4360 2582.1436
4177 646.7935 2583.1449
4178 646.7977 2583.1617
4179 647.0615 2584.2169
4180 863.4003 2587.1791
4181 864.4068 2590.1986
4182 864.4068 2590.1986
4183 864.7422 2591.2048
4184 866.1331 2595.3775
4190 866.4716 2596.3930
4191 866.4727 2596.3963
4192 650.2866 2597.1173
4194 868.0801 2601.2185
4195 868.4064 2602.1974
4197 876.7414 2627.2024
4198 883.4734 2647.3984
4199 883.7977 2648.3713
4200 884.4620 2650.3642
4201 897.1672 2688.4798
4202 898.4322 2692.2748
4203 898.4360 2692.2862
4204 898.4476 2692.3210
4205 906.8693 2717.5861
4208 910.7913 2729.3521
4209 917.8391 2750.4955
4210 917.8422 2750.5048
4211 918.1754 2751.5044
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