MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy1
MS data file : PRT1311_JBEI_1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 8 Sep 2025 at 17:17:19 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,450

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 301–400 (out of 4217)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
61 303.0848 302.0775
62 303.1022 302.0949
63 303.1026 302.0953
64 303.1216 302.1143
65 303.1216 302.1143
66 303.1216 302.1143
67 303.1216 302.1143
68 303.1216 302.1143
69 303.1217 302.1144
70 303.1217 302.1144
71 303.1219 302.1146
72 303.1219 302.1146
73 303.1219 302.1146
74 303.1219 302.1146
75 303.1219 302.1146
76 303.1219 302.1146
77 303.1220 302.1147
78 303.1220 302.1147
79 303.1223 302.1150
80 303.1609 302.1536
81 303.1784 302.1711
82 303.1788 302.1715
83 303.1788 302.1715
84 303.9641 302.9568
85 304.1764 303.1691
86 304.1769 303.1696
87 304.1770 303.1697
88 304.1771 303.1698
89 304.9723 303.9650
90 304.9724 303.9651
91 305.0988 304.0915
92 305.0988 304.0915
93 305.0992 304.0919
94 305.0999 304.0926
95 305.1000 304.0927
96 305.1003 304.0930
97 305.1006 304.0933
98 305.1007 304.0934
99 305.1007 304.0934
100 305.1007 304.0934
101 305.1008 304.0935
102 305.1008 304.0935
103 305.1010 304.0937
104 305.1011 304.0938
105 305.1375 304.1302
106 305.1375 304.1302
107 305.1376 304.1303
108 305.1377 304.1304
109 305.1377 304.1304
110 305.1377 304.1304
111 305.1377 304.1304
112 305.1377 304.1304
113 305.1378 304.1305
114 305.1378 304.1305
115 305.1378 304.1305
116 305.1378 304.1305
117 305.1378 304.1305
118 305.1378 304.1305
119 305.1378 304.1305
120 305.1378 304.1305
121 305.1379 304.1306
122 305.1379 304.1306
123 305.1379 304.1306
124 305.1381 304.1308
125 305.1385 304.1312
126 305.1583 304.1510
127 305.1743 304.1670
128 305.1744 304.1671
129 305.1745 304.1672
130 305.1745 304.1672
131 305.1745 304.1672
132 305.1746 304.1673
133 305.1746 304.1673
134 305.1747 304.1674
135 305.1747 304.1674
136 305.1747 304.1674
137 305.1748 304.1675
138 305.1750 304.1677
139 305.1753 304.1680
140 305.1753 304.1680
141 305.1754 304.1681
142 305.1755 304.1682
143 305.1756 304.1683
144 305.1757 304.1684
145 305.1758 304.1685
146 305.1759 304.1686
147 305.1762 304.1689
148 305.1962 304.1889
149 305.2109 304.2036
150 305.2119 304.2046
151 305.9581 304.9508
152 306.0060 304.9987
153 306.0061 304.9988
154 306.0061 304.9988
155 306.0062 304.9989
156 306.0064 304.9991
157 306.1557 305.1484
158 306.1561 305.1488
159 306.1561 305.1488
160 306.1561 305.1488
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