MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy1
MS data file : PRT1311_JBEI_1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 8 Sep 2025 at 17:17:19 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,450

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3801–3900 (out of 4217)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3939 706.7319 2117.1739
3940 706.7322 2117.1748
3941 706.7325 2117.1757
3942 706.7348 2117.1826
3943 707.0362 2118.0868
3944 1060.5081 2119.0016
3946 1060.5532 2119.0918
3947 531.2728 2121.0621
3949 708.0304 2121.0694
3950 531.2762 2121.0757
3953 708.3637 2122.0693
3954 531.5247 2122.0697
3955 531.5315 2122.0969
3956 708.6285 2122.8637
3957 710.0349 2127.0829
3962 535.2645 2137.0289
3964 713.4017 2137.1833
3965 713.4019 2137.1839
3966 713.4022 2137.1848
3967 713.4023 2137.1851
3968 713.4036 2137.1890
3970 535.5164 2138.0365
3971 713.6916 2138.0530
3974 715.7311 2144.1715
3975 716.0629 2145.1669
3976 716.3177 2145.9313
3977 716.6536 2146.9390
3979 718.0577 2151.1513
3980 1080.0172 2158.0198
3981 1080.5179 2159.0212
3986 1086.4120 2170.8094
3987 1088.0798 2174.1450
3988 727.7065 2180.0977
3989 727.7104 2180.1094
3990 727.7109 2180.1109
3991 728.0408 2181.1006
3993 1091.5619 2181.1092
3994 1091.5625 2181.1104
3995 1091.5627 2181.1108
3996 1091.5627 2181.1108
3997 1091.5629 2181.1112
3998 728.0446 2181.1120
3999 728.0447 2181.1123
4001 728.0462 2181.1168
4002 728.0464 2181.1174
4003 1091.5686 2181.1226
4004 737.3865 2209.1377
4005 737.7206 2210.1400
4011 738.0776 2211.2110
4012 1114.9064 2227.7982
4013 1115.2408 2228.4670
4014 560.0514 2236.1765
4015 747.3892 2239.1458
4016 747.7227 2240.1463
4017 571.5632 2282.2237
4018 571.5655 2282.2329
4019 762.0682 2283.1828
4022 765.0841 2292.2305
4024 765.4167 2293.2283
4026 765.4183 2293.2331
4027 765.4184 2293.2334
4028 765.4205 2293.2397
4030 1152.0597 2302.1048
4031 1152.5587 2303.1028
4032 768.7523 2303.2351
4033 1152.6278 2303.2410
4034 770.0405 2307.0997
4035 770.0422 2307.1048
4036 775.0873 2322.2401
4037 775.4229 2323.2469
4038 775.4229 2323.2469
4039 775.7181 2324.1325
4040 775.7194 2324.1364
4041 781.0938 2340.2596
4042 1171.1382 2340.2618
4043 781.0949 2340.2629
4044 781.0960 2340.2662
4045 586.0751 2340.2713
4046 781.3891 2341.1455
4048 781.3900 2341.1482
4049 586.3082 2341.2037
4050 1171.6285 2341.2424
4051 781.4247 2341.2523
4052 781.4249 2341.2529
4053 781.4255 2341.2547
4054 781.4260 2341.2562
4055 1171.6360 2341.2574
4056 781.4265 2341.2577
4057 586.3226 2341.2613
4059 1172.6293 2343.2440
4061 784.0858 2349.2356
4062 784.0884 2349.2434
4064 786.4249 2356.2529
4065 786.4252 2356.2538
4066 786.4335 2356.2787
4069 787.4102 2359.2088
4070 787.4132 2359.2178
4071 1181.1172 2360.2198
4072 591.8161 2363.2353
4073 592.3138 2365.2261
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