MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy1
MS data file : PRT1311_JBEI_1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 8 Sep 2025 at 17:17:19 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,450

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3701–3800 (out of 4217)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3782 844.8877 1687.7608
3783 563.5944 1687.7614
3788 581.0008 1739.9806
3789 581.3344 1740.9814
3790 871.5007 1740.9868
3791 871.9549 1741.8952
3792 884.6277 1767.2408
3793 442.9550 1767.7909
3794 591.2841 1770.8305
3795 591.2847 1770.8323
3796 886.9459 1771.8772
3797 887.4464 1772.8782
3798 887.4490 1772.8834
3799 892.1542 1782.2938
3800 898.4423 1794.8700
3804 904.4840 1806.9534
3807 605.3209 1812.9409
3808 921.4927 1840.9708
3809 616.3156 1845.9250
3810 616.3161 1845.9265
3811 927.9785 1853.9424
3812 618.9903 1853.9491
3813 929.9845 1857.9544
3814 934.0497 1866.0848
3815 623.0357 1866.0853
3816 623.0364 1866.0874
3817 934.5515 1867.0884
3818 937.3283 1872.6420
3819 941.4750 1880.9354
3820 941.4861 1880.9576
3821 953.4917 1904.9688
3822 955.5165 1909.0184
3823 956.5134 1911.0122
3824 958.9854 1915.9562
3826 645.0089 1932.0049
3829 645.3447 1933.0123
3830 645.3447 1933.0123
3838 652.6729 1954.9969
3840 654.9884 1961.9434
3841 982.0207 1962.0268
3842 982.0222 1962.0298
3843 982.0225 1962.0304
3844 982.0229 1962.0312
3845 655.0180 1962.0322
3846 655.3221 1962.9445
3850 492.2555 1964.9929
3851 492.5068 1965.9981
3853 1005.5098 2009.0050
3854 1007.9984 2013.9822
3855 1010.3377 2018.6608
3856 1010.6740 2019.3334
3857 1012.5200 2023.0254
3858 1012.5248 2023.0350
3859 1017.0267 2032.0388
3860 1024.5112 2047.0078
3861 1024.5319 2047.0492
3863 687.0206 2058.0400
3865 1030.5281 2059.0416
3866 687.3554 2059.0444
3867 688.0616 2061.1630
3868 689.3750 2065.1032
3874 1036.5338 2071.0530
3890 1045.9366 2089.8586
3891 697.9620 2090.8642
3892 1048.1893 2094.3640
3893 1048.5333 2095.0520
3894 1048.5369 2095.0592
3895 1050.5613 2099.1080
3896 700.7100 2099.1082
3897 1050.5646 2099.1146
3898 1050.5648 2099.1150
3899 700.7125 2099.1157
3901 700.7129 2099.1169
3902 1050.5674 2099.1202
3903 1050.9563 2099.8980
3904 700.9743 2099.9011
3906 700.9747 2099.9023
3907 1050.9585 2099.9024
3908 1051.5359 2101.0572
3910 701.3666 2101.0780
3911 701.6989 2102.0749
3912 1053.5573 2105.1000
3913 702.7075 2105.1007
3914 1053.5594 2105.1042
3915 1054.0308 2106.0470
3916 527.5307 2106.0937
3917 703.3026 2106.8860
3918 1054.4521 2106.8896
3919 527.7775 2107.0809
3921 527.7783 2107.0841
3922 703.3689 2107.0849
3923 703.3694 2107.0864
3925 1055.5494 2109.0842
3926 1056.5652 2111.1158
3927 1057.0613 2112.1080
3928 705.0435 2112.1087
3935 1059.5424 2117.0702
3936 1059.5477 2117.0808
3937 706.7011 2117.0815
3938 1059.5497 2117.0848
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