MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy1
MS data file : PRT1311_JBEI_1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 8 Sep 2025 at 17:17:19 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,450

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3601–3700 (out of 4217)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3591 712.8622 1423.7098
3592 712.8623 1423.7100
3593 475.5773 1423.7101
3596 475.5775 1423.7107
3602 712.8636 1423.7126
3604 712.8641 1423.7136
3605 713.3618 1424.7090
3606 475.9127 1424.7163
3607 479.2667 1434.7783
3608 479.2675 1434.7807
3609 479.2679 1434.7819
3610 479.2680 1434.7822
3611 480.2470 1437.7192
3612 720.8591 1439.7036
3619 483.5636 1447.6690
3622 483.5638 1447.6696
3623 724.8422 1447.6698
3624 483.5641 1447.6705
3636 485.2722 1452.7948
3637 485.2726 1452.7960
3639 730.8835 1459.7524
3640 366.4215 1461.6569
3641 488.2263 1461.6571
3642 488.2265 1461.6577
3643 488.2266 1461.6580
3644 488.2268 1461.6586
3645 488.2269 1461.6589
3646 488.2270 1461.6592
3649 741.8738 1481.7330
3650 741.8770 1481.7394
3651 741.8774 1481.7402
3652 742.3624 1482.7102
3653 742.3628 1482.7110
3654 742.3644 1482.7142
3657 745.4455 1488.8764
3658 745.4492 1488.8838
3659 373.4269 1489.6785
3661 373.4270 1489.6789
3662 497.5682 1489.6828
3663 498.5862 1492.7368
3666 499.6031 1495.7875
3672 377.4619 1505.8185
3673 377.4619 1505.8185
3677 377.4620 1505.8189
3686 377.4623 1505.8201
3688 377.4624 1505.8205
3689 377.7132 1506.8237
3691 757.4011 1512.7876
3692 505.2712 1512.7918
3694 507.6071 1519.7995
3695 507.6071 1519.7995
3696 507.9405 1520.7997
3697 383.4515 1529.7769
3700 512.2795 1533.8167
3701 513.6103 1537.8091
3702 513.6104 1537.8094
3703 513.6105 1537.8097
3704 514.3001 1539.8785
3707 772.8971 1543.7796
3709 772.8973 1543.7800
3711 515.6219 1543.8439
3712 777.4153 1552.8160
3714 518.6129 1552.8169
3715 518.6130 1552.8172
3716 777.4160 1552.8174
3717 518.6131 1552.8175
3718 518.6132 1552.8178
3719 518.6132 1552.8178
3720 518.6132 1552.8178
3721 518.6133 1552.8181
3722 518.6136 1552.8190
3724 518.9470 1553.8192
3725 518.9476 1553.8210
3730 521.9551 1562.8435
3733 785.4424 1568.8702
3734 786.4059 1570.7972
3738 527.6165 1579.8277
3739 527.6165 1579.8277
3740 527.6165 1579.8277
3742 791.4158 1580.8170
3746 797.3532 1592.6918
3747 804.9171 1607.8196
3749 536.9482 1607.8228
3750 537.6193 1609.8361
3751 405.1906 1616.7333
3753 539.9374 1616.7904
3754 405.4509 1617.7745
3761 544.6088 1630.8046
3762 544.6091 1630.8055
3766 553.3083 1656.9031
3767 553.3088 1656.9046
3768 553.6423 1657.9051
3769 416.4874 1661.9205
3770 416.7379 1662.9225
3775 837.5539 1673.0932
3776 558.9745 1673.9017
3777 419.4830 1673.9029
3778 842.9324 1683.8502
3780 844.8871 1687.7596
3781 844.8872 1687.7598
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