MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy1
MS data file : PRT1311_JBEI_1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 8 Sep 2025 at 17:17:19 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,450

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3501–3600 (out of 4217)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3426 1132.8790 1131.8717
3427 568.3149 1134.6152
3428 568.3151 1134.6156
3431 382.5398 1144.5976
3433 573.3071 1144.5996
3434 383.1721 1146.4945
3435 578.2299 1154.4452
3436 386.5578 1156.6516
3437 386.5579 1156.6519
3440 588.7814 1175.5482
3441 588.7818 1175.5490
3442 1179.3743 1178.3670
3443 1179.8744 1178.8671
3444 1181.6189 1180.6116
3445 394.5825 1180.7257
3449 595.3376 1188.6606
3450 397.5826 1189.7260
3451 397.9258 1190.7556
3452 397.9258 1190.7556
3453 397.9260 1190.7562
3454 397.9260 1190.7562
3455 397.9261 1190.7565
3456 397.9261 1190.7565
3457 596.3856 1190.7566
3458 596.3856 1190.7566
3459 596.3858 1190.7570
3460 397.9263 1190.7571
3461 397.9263 1190.7571
3462 397.9265 1190.7577
3463 397.9265 1190.7577
3464 600.8382 1199.6618
3465 600.8631 1199.7116
3466 402.5855 1204.7347
3467 402.5859 1204.7359
3468 402.5862 1204.7368
3469 609.7885 1217.5624
3470 407.2580 1218.7522
3471 407.2580 1218.7522
3472 407.2581 1218.7525
3473 407.2581 1218.7525
3474 611.7705 1221.5264
3475 611.7710 1221.5274
3476 408.5890 1222.7452
3477 408.5892 1222.7458
3478 408.5894 1222.7464
3479 408.5896 1222.7470
3480 408.5896 1222.7470
3481 410.1972 1227.5698
3482 410.1976 1227.5710
3486 308.9529 1231.7825
3487 308.9530 1231.7829
3488 411.6016 1231.7830
3493 617.7869 1233.5592
3494 412.2332 1233.6778
3498 620.7767 1239.5388
3501 416.9330 1247.7772
3502 312.9517 1247.7777
3503 312.9517 1247.7777
3504 416.9332 1247.7778
3505 416.9333 1247.7781
3506 416.9333 1247.7781
3507 312.9518 1247.7781
3508 312.9518 1247.7781
3509 312.9518 1247.7781
3510 417.8751 1250.6035
3511 632.3624 1262.7102
3512 632.3629 1262.7112
3513 632.8561 1263.6976
3514 632.8638 1263.7130
3515 634.2943 1266.5740
3516 634.2957 1266.5768
3517 635.3146 1268.6146
3518 635.3146 1268.6146
3519 635.3147 1268.6148
3520 635.3153 1268.6160
3521 635.3153 1268.6160
3534 639.8360 1277.6574
3536 642.3670 1282.7194
3537 642.3676 1282.7206
3538 642.3679 1282.7212
3540 432.9337 1295.7793
3541 432.9339 1295.7799
3542 650.3397 1298.6648
3544 651.8641 1301.7136
3545 651.8655 1301.7164
3550 654.8389 1307.6632
3552 439.5403 1315.5991
3553 441.5726 1321.6960
3555 441.5728 1321.6966
3557 441.5729 1321.6969
3563 665.3710 1328.7274
3564 444.5437 1330.6093
3565 444.8777 1331.6113
3569 451.1669 1350.4789
3573 693.3581 1384.7016
3574 693.3585 1384.7024
3576 465.2513 1392.7321
3582 712.8618 1423.7090
3583 712.8618 1423.7090
3589 712.8621 1423.7096
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