MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy1
MS data file : PRT1311_JBEI_1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 8 Sep 2025 at 17:17:19 GMT
Repeat search

[help]

Export

Not what you expected? Try the select summary.

Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,450

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

Filters

[help]

Show

Unassigned peptides, 3401–3500 (out of 4217)

Page: Previous 1 …30 31 32 33 34 35 36 37 38 39 40 … 43 Next 

Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3258 478.7356 955.4566
3260 322.5075 964.5007
3261 967.4960 966.4887
3263 486.2411 970.4676
3266 972.4838 971.4765
3267 487.2480 972.4814
3268 487.2485 972.4824
3271 325.5362 973.5868
3272 325.5362 973.5868
3274 327.1213 978.3421
3276 327.2080 978.6022
3277 490.3087 978.6028
3278 490.3087 978.6028
3279 490.3087 978.6028
3280 490.3088 978.6030
3281 327.2083 978.6031
3282 491.7904 981.5662
3284 491.7907 981.5668
3288 492.2679 982.5212
3293 328.5145 982.5217
3295 495.2593 988.5040
3297 331.1811 990.5215
3308 993.5089 992.5016
3310 1003.6646 1002.6573
3311 1010.5071 1009.4998
3312 1011.0134 1010.0061
3314 506.7811 1011.5476
3316 506.7815 1011.5484
3317 506.7816 1011.5486
3318 1015.5093 1014.5020
3319 1018.5204 1017.5131
3320 510.8039 1019.5932
3321 510.8042 1019.5938
3322 510.8045 1019.5944
3323 512.8014 1023.5882
3324 512.8018 1023.5890
3325 512.8018 1023.5890
3326 1026.2178 1025.2105
3335 345.8919 1034.6539
3339 518.3350 1034.6554
3341 346.9016 1037.6830
3342 348.2036 1041.5890
3346 523.2927 1044.5708
3349 350.5470 1048.6192
3350 350.5471 1048.6195
3351 350.5471 1048.6195
3352 525.3172 1048.6198
3353 525.3173 1048.6200
3354 1051.5325 1050.5252
3355 1056.0354 1055.0281
3356 1060.5408 1059.5335
3357 1061.5372 1060.5299
3360 355.5435 1063.6087
3361 533.2681 1064.5216
3362 358.1943 1071.5611
3363 358.1953 1071.5641
3364 358.1956 1071.5650
3365 537.2863 1072.5580
3368 358.5424 1072.6054
3370 360.2305 1077.6697
3371 360.2310 1077.6712
3372 360.2310 1077.6712
3373 360.2310 1077.6712
3374 360.2311 1077.6715
3375 539.8431 1077.6716
3376 539.8436 1077.6726
3377 539.8438 1077.6730
3378 1079.5488 1078.5415
3379 1081.5554 1080.5481
3382 362.1941 1083.5605
3386 1092.0604 1091.0531
3387 546.8460 1091.6774
3388 547.2749 1092.5352
3390 1093.5454 1092.5381
3396 547.2775 1092.5404
3400 550.8041 1099.5936
3401 550.8043 1099.5940
3402 554.2787 1106.5428
3403 370.0286 1107.0640
3404 370.8844 1109.6314
3405 370.8849 1109.6329
3406 1113.5927 1112.5854
3407 372.8584 1115.5534
3408 559.2564 1116.4982
3410 559.2568 1116.4990
3411 559.2571 1116.4996
3412 559.2578 1116.5010
3413 559.3056 1116.5966
3414 559.8093 1117.6040
3415 373.9998 1118.9776
3416 377.1226 1128.3460
3417 377.2176 1128.6310
3418 377.2179 1128.6319
3419 566.2503 1130.4860
3420 566.2506 1130.4866
3421 377.8365 1130.4877
3422 377.8365 1130.4877
3423 566.2513 1130.4880
3424 566.2513 1130.4880
3425 566.2513 1130.4880
Page: Previous 1 …30 31 32 33 34 35 36 37 38 39 40 … 43 Next 

Not what you expected? Try the select summary.

Mascot: http://www.matrixscience.com/