MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy1
MS data file : PRT1311_JBEI_1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 8 Sep 2025 at 17:17:19 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,450

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3301–3400 (out of 4217)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
3069 620.2850 619.2777
3070 620.2864 619.2791
3072 623.3262 622.3189
3073 312.6653 623.3160
3074 312.6657 623.3168
3075 317.1615 632.3084
3076 636.9379 635.9306
3077 638.3765 637.3692
3079 638.3767 637.3694
3080 319.6920 637.3694
3082 638.3770 637.3697
3086 643.1226 642.1153
3087 645.0975 644.0902
3090 324.0167 646.0188
3091 324.0168 646.0190
3092 324.0172 646.0198
3093 325.1638 648.3130
3094 325.1642 648.3138
3095 330.0481 658.0816
3096 332.2086 662.4026
3097 333.9640 665.9134
3098 667.3011 666.2938
3102 667.3023 666.2950
3103 672.4078 671.4005
3104 674.0534 673.0461
3105 339.1035 676.1924
3116 341.1761 680.3376
3120 354.1971 706.3796
3121 354.6882 707.3618
3122 354.7016 707.3886
3123 354.7017 707.3888
3125 354.7019 707.3892
3126 712.8621 711.8548
3127 740.8693 739.8620
3129 743.4453 742.4380
3130 744.9899 743.9826
3134 376.2343 750.4540
3136 768.4188 767.4115
3140 392.2294 782.4442
3142 783.7496 782.7423
3143 393.2298 784.4450
3146 786.4034 785.3961
3147 787.0007 785.9934
3148 789.4834 788.4761
3149 798.3950 797.3877
3150 798.4035 797.3962
3151 799.4113 798.4040
3155 401.2483 800.4820
3160 404.7447 807.4748
3161 404.7449 807.4752
3162 404.7451 807.4756
3163 404.7451 807.4756
3164 404.7455 807.4764
3165 404.7456 807.4766
3166 814.4449 813.4376
3171 408.7401 815.4656
3172 819.4158 818.4085
3173 412.7553 823.4960
3174 412.7556 823.4966
3187 859.0757 858.0684
3189 433.2558 864.4970
3190 866.1395 865.1322
3193 435.7761 869.5376
3194 435.7761 869.5376
3195 435.7763 869.5380
3197 436.8563 871.6980
3199 890.2350 889.2277
3200 891.4097 890.4024
3202 301.0871 900.2395
3203 901.4542 900.4469
3204 901.4608 900.4535
3210 914.5382 913.5309
3211 457.7738 913.5330
3212 458.2749 914.5352
3219 307.8607 920.5603
3220 307.8607 920.5603
3221 461.2877 920.5608
3222 461.2878 920.5610
3223 461.2879 920.5612
3224 461.2879 920.5612
3225 461.7922 921.5698
3227 309.5014 925.4824
3228 309.8374 926.4904
3229 464.7378 927.4610
3230 464.7606 927.5066
3231 932.4883 931.4810
3232 932.4977 931.4904
3233 313.1790 936.5152
3234 938.5140 937.5067
3235 314.1616 939.4630
3236 314.1618 939.4636
3237 472.7355 943.4564
3238 472.7358 943.4570
3239 472.7359 943.4572
3240 944.6252 943.6179
3244 953.4850 952.4777
3245 954.2355 953.2282
3246 954.4949 953.4876
3247 955.4825 954.4752
3257 955.9185 954.9112
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