MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy1
MS data file : PRT1311_JBEI_1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 8 Sep 2025 at 17:17:19 GMT
Repeat search

[help]

Export

Not what you expected? Try the select summary.

Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,450

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

Filters

[help]

Show

Unassigned peptides, 3201–3300 (out of 4217)

Page: Previous 1 …28 29 30 31 32 33 34 35 36 37 38 … 43 Next 

Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2961 492.9069 491.8996
2962 493.0202 492.0129
2963 493.2426 492.2353
2964 493.2443 492.2370
2965 493.2446 492.2373
2966 494.0505 493.0432
2967 494.0514 493.0441
2968 494.9068 493.8995
2969 496.1070 495.0997
2970 496.1077 495.1004
2971 496.1080 495.1007
2972 497.1987 496.1914
2973 497.1989 496.1916
2974 499.1774 498.1701
2975 499.1777 498.1704
2976 499.1778 498.1705
2977 500.7596 499.7523
2978 503.1102 502.1029
2979 504.8371 503.8298
2980 504.8372 503.8299
2981 510.1238 509.1165
2982 510.2408 509.2335
2983 510.2416 509.2343
2984 510.2416 509.2343
2985 511.1786 510.1713
2986 511.1786 510.1713
2987 513.1931 512.1858
2988 513.1933 512.1860
2989 513.1940 512.1867
2990 515.3327 514.3254
2991 520.2325 519.2252
2992 520.2329 519.2256
2993 520.2329 519.2256
2994 520.2329 519.2256
2995 520.2330 519.2257
2996 522.1249 521.1176
2997 522.1250 521.1177
2998 522.1251 521.1178
2999 523.1013 522.0940
3000 523.1025 522.0952
3001 525.1945 524.1872
3002 525.2816 524.2743
3003 528.0560 527.0487
3004 529.0398 528.0325
3005 532.1270 531.1197
3006 532.1277 531.1204
3010 537.1176 536.1103
3011 538.1186 537.1113
3012 539.2103 538.2030
3015 539.3086 538.3013
3016 541.1892 540.1819
3017 541.2925 540.2852
3018 542.2856 541.2783
3019 542.2859 541.2786
3020 542.2866 541.2793
3021 542.2869 541.2796
3023 551.2965 550.2892
3024 551.2969 550.2896
3025 552.1340 551.1267
3026 552.1347 551.1274
3027 552.1349 551.1276
3028 552.2699 551.2626
3029 552.2700 551.2627
3030 552.2701 551.2628
3031 552.2703 551.2630
3032 552.2706 551.2633
3033 555.0303 554.0230
3034 555.0305 554.0232
3035 555.2059 554.1986
3036 557.0105 556.0032
3037 562.0452 561.0379
3038 566.1174 565.1101
3039 566.1186 565.1113
3040 566.1187 565.1114
3041 567.1291 566.1218
3042 579.2920 578.2847
3043 579.2921 578.2848
3045 582.1130 581.1057
3046 586.0629 585.0556
3047 589.3703 588.3630
3048 300.5289 599.0432
3049 601.3713 600.3640
3050 603.0892 602.0819
3051 302.5362 603.0578
3052 604.0958 603.0885
3053 606.0706 605.0633
3054 606.0709 605.0636
3055 308.1563 614.2980
3056 308.1962 614.3778
3058 616.2897 615.2824
3059 616.2906 615.2833
3060 616.2907 615.2834
3061 616.2911 615.2838
3062 309.0422 616.0698
3063 309.0424 616.0702
3064 309.0938 616.1730
3065 309.1693 616.3240
3066 309.1696 616.3246
3067 310.5336 619.0526
3068 310.5339 619.0532
Page: Previous 1 …28 29 30 31 32 33 34 35 36 37 38 … 43 Next 

Not what you expected? Try the select summary.

Mascot: http://www.matrixscience.com/