MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy1
MS data file : PRT1311_JBEI_1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 8 Sep 2025 at 17:17:19 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,450

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3101–3200 (out of 4217)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2861 451.2336 450.2263
2862 451.2341 450.2268
2863 451.2342 450.2269
2864 453.1729 452.1656
2865 453.1731 452.1658
2866 453.1736 452.1663
2867 453.2115 452.2042
2868 453.2117 452.2044
2869 453.2119 452.2046
2870 453.2122 452.2049
2871 453.2122 452.2049
2872 453.2122 452.2049
2873 453.2123 452.2050
2874 453.2124 452.2051
2875 453.2124 452.2051
2876 453.2125 452.2052
2877 453.2127 452.2054
2878 453.2127 452.2054
2879 453.2129 452.2056
2880 455.1510 454.1437
2881 455.1524 454.1451
2882 455.1891 454.1818
2883 455.1892 454.1819
2884 455.2280 454.2207
2885 455.2284 454.2211
2886 455.2704 454.2631
2887 455.2704 454.2631
2888 455.2707 454.2634
2889 455.2708 454.2635
2890 455.2709 454.2636
2891 455.2711 454.2638
2892 455.2711 454.2638
2893 455.2711 454.2638
2894 455.2711 454.2638
2895 457.1659 456.1586
2896 457.1679 456.1606
2897 461.2591 460.2518
2898 462.0878 461.0805
2899 463.0799 462.0726
2900 464.0279 463.0206
2901 465.0956 464.0883
2902 465.0973 464.0900
2903 465.2120 464.2047
2904 465.2121 464.2048
2905 465.2121 464.2048
2906 465.2124 464.2051
2907 465.2125 464.2052
2908 465.2126 464.2053
2909 465.2127 464.2054
2910 465.2130 464.2057
2911 465.2131 464.2058
2912 466.0432 465.0359
2913 466.0440 465.0367
2914 466.0975 465.0902
2915 467.1881 466.1808
2916 467.1911 466.1838
2917 467.2280 466.2207
2918 467.2284 466.2211
2919 467.2285 466.2212
2920 467.2286 466.2213
2921 467.2286 466.2213
2922 467.2286 466.2213
2923 467.2288 466.2215
2924 467.2295 466.2222
2925 467.2295 466.2222
2926 469.0187 468.0114
2927 469.1668 468.1595
2928 469.1670 468.1597
2929 469.1674 468.1601
2930 469.2067 468.1994
2931 469.2433 468.2360
2932 469.2434 468.2361
2933 469.2437 468.2364
2934 469.2443 468.2370
2935 471.1362 470.1289
2936 471.1821 470.1748
2937 472.2908 471.2835
2938 473.0509 472.0436
2939 473.1518 472.1445
2940 473.1614 472.1541
2941 476.1011 475.0938
2942 476.1012 475.0939
2943 476.9344 475.9271
2944 479.0833 478.0760
2945 479.2282 478.2209
2946 480.1125 479.1052
2947 480.1132 479.1059
2948 481.0922 480.0849
2949 481.1665 480.1592
2950 481.2066 480.1993
2951 481.2072 480.1999
2952 481.2440 480.2367
2953 483.1825 482.1752
2954 483.1830 482.1757
2955 483.1832 482.1759
2956 485.1614 484.1541
2957 485.1618 484.1545
2958 487.0300 486.0227
2959 487.3377 486.3304
2960 490.0905 489.0832
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