MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy1
MS data file : PRT1311_JBEI_1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 8 Sep 2025 at 17:17:19 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,450

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3001–3100 (out of 4217)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2761 437.2173 436.2100
2762 437.2173 436.2100
2763 437.2173 436.2100
2764 437.2175 436.2102
2765 437.2175 436.2102
2766 437.2175 436.2102
2767 437.2177 436.2104
2768 437.2177 436.2104
2769 437.2177 436.2104
2770 437.2185 436.2112
2771 439.1564 438.1491
2772 439.1567 438.1494
2773 439.1574 438.1501
2774 439.1953 438.1880
2775 439.1959 438.1886
2776 439.1962 438.1889
2777 439.1964 438.1891
2778 439.1965 438.1892
2779 439.1968 438.1895
2780 439.1970 438.1897
2781 439.1971 438.1898
2782 439.1972 438.1899
2783 439.1973 438.1900
2784 439.1973 438.1900
2785 439.2148 438.2075
2786 439.2150 438.2077
2787 439.2150 438.2077
2788 439.2150 438.2077
2789 439.2151 438.2078
2790 439.2151 438.2078
2791 439.2152 438.2079
2792 439.2154 438.2081
2793 439.2155 438.2082
2794 439.2158 438.2085
2795 439.2162 438.2089
2796 439.2330 438.2257
2797 439.2330 438.2257
2798 439.2330 438.2257
2799 439.2330 438.2257
2800 439.2330 438.2257
2801 439.2336 438.2263
2802 439.9673 438.9600
2803 440.2388 439.2315
2804 440.2389 439.2316
2805 441.1729 440.1656
2806 441.1731 440.1658
2807 441.1732 440.1659
2808 441.2122 440.2049
2809 441.2305 440.2232
2810 441.2307 440.2234
2811 441.2310 440.2237
2812 441.2310 440.2237
2813 441.2313 440.2240
2814 441.2323 440.2250
2815 441.2331 440.2258
2816 443.0435 442.0362
2817 443.1487 442.1414
2818 443.1504 442.1431
2819 443.1530 442.1457
2820 443.2467 442.2394
2821 443.2468 442.2395
2822 443.2473 442.2400
2823 443.2474 442.2401
2824 443.2476 442.2403
2825 443.2487 442.2414
2826 445.0191 444.0118
2827 445.0195 444.0122
2828 446.2994 445.2921
2829 446.2999 445.2926
2830 446.3001 445.2928
2831 449.0906 448.0833
2832 449.1526 448.1453
2833 449.1541 448.1468
2834 449.1803 448.1730
2835 449.1809 448.1736
2836 449.1815 448.1742
2837 449.2173 448.2100
2838 449.2173 448.2100
2839 449.2174 448.2101
2840 449.2174 448.2101
2841 449.2176 448.2103
2842 450.2476 449.2403
2843 450.2480 449.2407
2844 450.2484 449.2411
2845 451.1567 450.1494
2846 451.1689 450.1616
2847 451.1700 450.1627
2848 451.1952 450.1879
2849 451.1961 450.1888
2850 451.1963 450.1890
2851 451.1964 450.1891
2852 451.1965 450.1892
2853 451.1967 450.1894
2854 451.1969 450.1896
2855 451.1971 450.1898
2856 451.1971 450.1898
2857 451.1972 450.1899
2858 451.1974 450.1901
2859 451.2331 450.2258
2860 451.2332 450.2259
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