MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy1
MS data file : PRT1311_JBEI_1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 8 Sep 2025 at 17:17:19 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,450

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2901–3000 (out of 4217)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2661 425.1805 424.1732
2662 425.1805 424.1732
2663 425.1806 424.1733
2664 425.1806 424.1733
2665 425.1807 424.1734
2666 425.1808 424.1735
2667 425.1810 424.1737
2668 425.1811 424.1738
2669 425.1814 424.1741
2670 425.2171 424.2098
2671 425.2171 424.2098
2672 425.2172 424.2099
2673 425.2173 424.2100
2674 425.2173 424.2100
2675 425.2173 424.2100
2676 425.2177 424.2104
2677 425.2178 424.2105
2678 425.2179 424.2106
2679 425.2179 424.2106
2680 425.2182 424.2109
2681 425.2185 424.2112
2682 425.2356 424.2283
2683 425.2357 424.2284
2684 425.2358 424.2285
2685 425.2359 424.2286
2686 425.2359 424.2286
2687 425.2361 424.2288
2688 425.2362 424.2289
2689 425.2362 424.2289
2690 425.2363 424.2290
2691 425.2370 424.2297
2692 425.2375 424.2302
2693 425.2903 424.2830
2694 426.0055 424.9982
2695 427.0126 426.0053
2696 427.1554 426.1481
2697 427.1561 426.1488
2698 427.1570 426.1497
2699 427.1957 426.1884
2700 427.1965 426.1892
2701 427.1969 426.1896
2702 429.0900 428.0827
2703 429.0903 428.0830
2704 429.0911 428.0838
2705 429.0912 428.0839
2706 429.0912 428.0839
2707 429.0913 428.0840
2708 429.0916 428.0843
2709 429.0917 428.0844
2710 429.0917 428.0844
2711 429.0918 428.0845
2712 429.0920 428.0847
2713 429.0921 428.0848
2714 429.1336 428.1263
2715 429.1723 428.1650
2716 429.2699 428.2626
2717 430.8563 429.8490
2718 431.0197 430.0124
2719 431.0207 430.0134
2720 431.2744 430.2671
2721 432.8508 431.8435
2722 433.0582 432.0509
2723 433.1858 432.1785
2724 433.1860 432.1787
2725 433.1867 432.1794
2726 435.0741 434.0668
2727 435.0760 434.0687
2728 435.2013 434.1940
2729 435.2013 434.1940
2730 435.2016 434.1943
2731 435.2016 434.1943
2732 435.2017 434.1944
2733 435.2018 434.1945
2734 435.2019 434.1946
2735 435.2020 434.1947
2736 435.2021 434.1948
2737 435.2021 434.1948
2738 435.2021 434.1948
2739 435.2023 434.1950
2740 435.2026 434.1953
2741 437.0878 436.0805
2742 437.0895 436.0822
2743 437.0901 436.0828
2744 437.1788 436.1715
2745 437.1795 436.1722
2746 437.1798 436.1725
2747 437.1801 436.1728
2748 437.1803 436.1730
2749 437.1804 436.1731
2750 437.1805 436.1732
2751 437.1808 436.1735
2752 437.1810 436.1737
2753 437.1811 436.1738
2754 437.1812 436.1739
2755 437.1987 436.1914
2756 437.2169 436.2096
2757 437.2171 436.2098
2758 437.2171 436.2098
2759 437.2172 436.2099
2760 437.2172 436.2099
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