MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy1
MS data file : PRT1311_JBEI_1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 8 Sep 2025 at 17:17:19 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,450

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2801–2900 (out of 4217)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2561 419.3185 418.3112
2562 419.3185 418.3112
2563 419.3186 418.3113
2564 419.3187 418.3114
2565 419.3188 418.3115
2566 419.3189 418.3116
2567 420.0742 419.0669
2568 420.9341 419.9268
2569 421.0586 420.0513
2570 421.0596 420.0523
2571 421.1855 420.1782
2572 421.1855 420.1782
2573 421.1857 420.1784
2574 421.1858 420.1785
2575 421.1859 420.1786
2576 421.1859 420.1786
2577 421.1860 420.1787
2578 421.1860 420.1787
2579 421.1863 420.1790
2580 421.1863 420.1790
2581 421.1865 420.1792
2582 421.1867 420.1794
2583 421.1872 420.1799
2584 421.2041 420.1968
2585 421.2042 420.1969
2586 421.2042 420.1969
2587 421.2042 420.1969
2588 421.2045 420.1972
2589 421.2046 420.1973
2590 421.2047 420.1974
2591 421.2209 420.2136
2592 421.2213 420.2140
2593 421.2213 420.2140
2594 421.2214 420.2141
2595 421.2216 420.2143
2596 421.2226 420.2153
2597 421.2227 420.2154
2598 421.2227 420.2154
2599 421.2228 420.2155
2600 421.2228 420.2155
2601 421.2228 420.2155
2602 421.2229 420.2156
2603 421.2231 420.2158
2604 421.2232 420.2159
2605 422.9301 421.9228
2606 423.0720 422.0647
2607 423.1620 422.1547
2608 423.1639 422.1566
2609 423.1643 422.1570
2610 423.1646 422.1573
2611 423.1647 422.1574
2612 423.1647 422.1574
2613 423.1651 422.1578
2614 423.1652 422.1579
2615 423.1653 422.1580
2616 423.1656 422.1583
2617 423.1999 422.1926
2618 423.2004 422.1931
2619 423.2005 422.1932
2620 423.2006 422.1933
2621 423.2007 422.1934
2622 423.2008 422.1935
2623 423.2009 422.1936
2624 423.2014 422.1941
2625 423.2014 422.1941
2626 423.2014 422.1941
2627 423.2014 422.1941
2628 423.2015 422.1942
2629 423.2015 422.1942
2630 423.2015 422.1942
2631 423.2015 422.1942
2632 423.2016 422.1943
2633 423.2016 422.1943
2634 423.2016 422.1943
2635 423.2016 422.1943
2636 423.2017 422.1944
2637 423.2018 422.1945
2638 423.2018 422.1945
2639 423.2020 422.1947
2640 423.2199 422.2126
2641 423.2199 422.2126
2642 423.2200 422.2127
2643 423.2201 422.2128
2644 423.2201 422.2128
2645 423.2202 422.2129
2646 423.2203 422.2130
2647 423.2203 422.2130
2648 423.2205 422.2132
2649 423.2205 422.2132
2650 423.2205 422.2132
2651 423.2206 422.2133
2652 423.2207 422.2134
2653 423.2216 422.2143
2654 423.2219 422.2146
2655 425.1394 424.1321
2656 425.1400 424.1327
2657 425.1787 424.1714
2658 425.1787 424.1714
2659 425.1797 424.1724
2660 425.1798 424.1725
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