MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy1
MS data file : PRT1311_JBEI_1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 8 Sep 2025 at 17:17:19 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,450

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2701–2800 (out of 4217)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2461 409.1860 408.1787
2462 409.1860 408.1787
2463 409.1862 408.1789
2464 409.1862 408.1789
2465 409.1862 408.1789
2466 409.1866 408.1793
2467 409.2226 408.2153
2468 409.2227 408.2154
2469 409.2227 408.2154
2470 409.2228 408.2155
2471 409.2230 408.2157
2472 409.2230 408.2157
2473 409.2405 408.2332
2474 409.2405 408.2332
2475 409.2406 408.2333
2476 409.2408 408.2335
2477 409.2411 408.2338
2478 409.2413 408.2340
2479 409.2420 408.2347
2480 409.2428 408.2355
2481 409.9915 408.9842
2482 410.2436 409.2363
2483 410.2437 409.2364
2484 410.2438 409.2365
2485 410.2443 409.2370
2486 411.0130 410.0057
2487 411.0133 410.0060
2488 411.1234 410.1161
2489 411.1625 410.1552
2490 411.1625 410.1552
2491 411.1635 410.1562
2492 411.1639 410.1566
2493 411.1649 410.1576
2494 411.1649 410.1576
2495 411.1650 410.1577
2496 411.1650 410.1577
2497 411.1651 410.1578
2498 411.1656 410.1583
2499 411.2011 410.1938
2500 411.2012 410.1939
2501 411.2013 410.1940
2502 411.2013 410.1940
2503 411.2014 410.1941
2504 411.2015 410.1942
2505 411.2018 410.1945
2506 411.2021 410.1948
2507 413.1394 412.1321
2508 413.1398 412.1325
2509 413.1800 412.1727
2510 413.1807 412.1734
2511 413.1809 412.1736
2512 413.1814 412.1741
2513 413.1814 412.1741
2514 413.2687 412.2614
2515 413.2691 412.2618
2516 413.2691 412.2618
2517 413.2694 412.2621
2518 413.2694 412.2621
2519 413.2696 412.2623
2520 413.2699 412.2626
2521 413.2700 412.2627
2522 413.2701 412.2628
2523 413.2703 412.2630
2524 413.2704 412.2631
2525 413.2720 412.2647
2526 413.2744 412.2671
2527 415.1560 414.1487
2528 415.1563 414.1490
2529 415.1575 414.1502
2530 415.2162 414.2089
2531 416.2663 415.2590
2532 416.3751 415.3678
2533 416.3756 415.3683
2534 417.0583 416.0510
2535 417.0589 416.0516
2536 417.1914 416.1841
2537 417.8750 416.8677
2538 419.1700 418.1627
2539 419.1700 418.1627
2540 419.1701 418.1628
2541 419.1701 418.1628
2542 419.1701 418.1628
2543 419.1704 418.1631
2544 419.2060 418.1987
2545 419.2064 418.1991
2546 419.2067 418.1994
2547 419.2067 418.1994
2548 419.2068 418.1995
2549 419.2068 418.1995
2550 419.2069 418.1996
2551 419.3170 418.3097
2552 419.3173 418.3100
2553 419.3175 418.3102
2554 419.3179 418.3106
2555 419.3179 418.3106
2556 419.3179 418.3106
2557 419.3180 418.3107
2558 419.3181 418.3108
2559 419.3183 418.3110
2560 419.3183 418.3110
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