MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy1
MS data file : PRT1311_JBEI_1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 8 Sep 2025 at 17:17:19 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,450

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2601–2700 (out of 4217)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2361 403.1752 402.1679
2362 403.1753 402.1680
2363 403.1757 402.1684
2364 403.2314 402.2241
2365 405.0741 404.0668
2366 405.0759 404.0686
2367 405.1541 404.1468
2368 405.1543 404.1470
2369 405.1543 404.1470
2370 405.1544 404.1471
2371 405.1550 404.1477
2372 405.1905 404.1832
2373 405.1906 404.1833
2374 405.1910 404.1837
2375 405.1911 404.1838
2376 405.1911 404.1838
2377 405.1912 404.1839
2378 405.1913 404.1840
2379 405.1913 404.1840
2380 405.1914 404.1841
2381 405.1914 404.1841
2382 405.1916 404.1843
2383 405.1916 404.1843
2384 405.1925 404.1852
2385 405.2269 404.2196
2386 405.2271 404.2198
2387 405.2275 404.2202
2388 405.2275 404.2202
2389 405.2276 404.2203
2390 405.2276 404.2203
2391 405.2277 404.2204
2392 405.2297 404.2224
2393 406.0584 405.0511
2394 406.0585 405.0512
2395 407.1697 406.1624
2396 407.1703 406.1630
2397 407.1704 406.1631
2398 407.1704 406.1631
2399 407.1705 406.1632
2400 407.1705 406.1632
2401 407.1705 406.1632
2402 407.1705 406.1632
2403 407.1705 406.1632
2404 407.1706 406.1633
2405 407.1707 406.1634
2406 407.1709 406.1636
2407 407.2066 406.1993
2408 407.2067 406.1994
2409 407.2068 406.1995
2410 407.2068 406.1995
2411 407.2069 406.1996
2412 407.2069 406.1996
2413 407.2069 406.1996
2414 407.2069 406.1996
2415 407.2069 406.1996
2416 407.2070 406.1997
2417 407.2071 406.1998
2418 407.2071 406.1998
2419 407.2076 406.2003
2420 407.2081 406.2008
2421 407.2084 406.2011
2422 407.2084 406.2011
2423 407.2087 406.2014
2424 407.2248 406.2175
2425 407.2248 406.2175
2426 407.2249 406.2176
2427 407.2249 406.2176
2428 407.2250 406.2177
2429 407.2251 406.2178
2430 407.2252 406.2179
2431 407.2252 406.2179
2432 407.2253 406.2180
2433 407.2253 406.2180
2434 407.2255 406.2182
2435 407.2255 406.2182
2436 407.2262 406.2189
2437 407.2272 406.2199
2438 407.2426 406.2353
2439 409.1459 408.1386
2440 409.1461 408.1388
2441 409.1472 408.1399
2442 409.1479 408.1406
2443 409.1494 408.1421
2444 409.1494 408.1421
2445 409.1495 408.1422
2446 409.1495 408.1422
2447 409.1497 408.1424
2448 409.1499 408.1426
2449 409.1657 408.1584
2450 409.1668 408.1595
2451 409.1852 408.1779
2452 409.1855 408.1782
2453 409.1857 408.1784
2454 409.1857 408.1784
2455 409.1858 408.1785
2456 409.1858 408.1785
2457 409.1859 408.1786
2458 409.1859 408.1786
2459 409.1859 408.1786
2460 409.1860 408.1787
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