MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy1
MS data file : PRT1311_JBEI_1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 8 Sep 2025 at 17:17:19 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,450

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2501–2600 (out of 4217)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2261 393.3003 392.2930
2262 393.3003 392.2930
2263 393.3004 392.2931
2264 393.3004 392.2931
2265 393.3004 392.2931
2266 393.3008 392.2935
2267 393.3020 392.2947
2268 395.0174 394.0101
2269 395.0178 394.0105
2270 395.0179 394.0106
2271 395.0362 394.0289
2272 395.0756 394.0683
2273 395.0757 394.0684
2274 395.0760 394.0687
2275 395.0768 394.0695
2276 395.1295 394.1222
2277 395.1297 394.1224
2278 395.1305 394.1232
2279 395.1317 394.1244
2280 395.1324 394.1251
2281 395.1687 394.1614
2282 395.1699 394.1626
2283 395.1699 394.1626
2284 395.1700 394.1627
2285 395.1701 394.1628
2286 395.1701 394.1628
2287 395.1702 394.1629
2288 395.1702 394.1629
2289 395.1702 394.1629
2290 395.1702 394.1629
2291 395.1703 394.1630
2292 395.1703 394.1630
2293 395.1704 394.1631
2294 395.1705 394.1632
2295 395.1706 394.1633
2296 395.1707 394.1634
2297 395.1708 394.1635
2298 395.2062 394.1989
2299 395.2064 394.1991
2300 395.2065 394.1992
2301 395.2065 394.1992
2302 395.2067 394.1994
2303 395.2067 394.1994
2304 395.2067 394.1994
2305 395.2068 394.1995
2306 395.2068 394.1995
2307 395.2070 394.1997
2308 395.2073 394.2000
2309 395.2073 394.2000
2310 395.2074 394.2001
2311 395.2077 394.2004
2312 395.2078 394.2005
2313 395.2250 394.2177
2314 395.2272 394.2199
2315 395.2614 394.2541
2316 395.2617 394.2544
2317 396.2644 395.2571
2318 396.2652 395.2579
2319 396.2663 395.2590
2320 397.0516 396.0443
2321 397.0518 396.0445
2322 397.0519 396.0446
2323 397.0522 396.0449
2324 397.1457 396.1384
2325 397.1463 396.1390
2326 397.1465 396.1392
2327 397.1475 396.1402
2328 397.1488 396.1415
2329 397.1495 396.1422
2330 397.1498 396.1425
2331 397.1855 396.1782
2332 397.1856 396.1783
2333 397.1857 396.1784
2334 397.1859 396.1786
2335 397.1859 396.1786
2336 397.1860 396.1787
2337 397.1860 396.1787
2338 397.1860 396.1787
2339 397.1860 396.1787
2340 397.1862 396.1789
2341 397.1862 396.1789
2342 397.1862 396.1789
2343 397.1866 396.1793
2344 397.1866 396.1793
2345 397.2219 396.2146
2346 397.2224 396.2151
2347 397.2234 396.2161
2348 399.1235 398.1162
2349 399.1242 398.1169
2350 399.1621 398.1548
2351 399.1630 398.1557
2352 399.2021 398.1948
2353 399.2026 398.1953
2354 401.1390 400.1317
2355 401.1397 400.1324
2356 402.2477 401.2404
2357 402.2478 401.2405
2358 402.2481 401.2408
2359 403.1750 402.1677
2360 403.1750 402.1677
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