MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy1
MS data file : PRT1311_JBEI_1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 8 Sep 2025 at 17:17:19 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,450

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2401–2500 (out of 4217)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2161 391.2864 390.2791
2162 391.2864 390.2791
2163 391.2864 390.2791
2164 391.2864 390.2791
2165 391.2864 390.2791
2166 391.2864 390.2791
2167 391.2864 390.2791
2168 391.2865 390.2792
2169 391.2865 390.2792
2170 391.2865 390.2792
2171 391.2865 390.2792
2172 391.2865 390.2792
2173 391.2866 390.2793
2174 391.2866 390.2793
2175 391.2866 390.2793
2176 391.2866 390.2793
2177 391.2866 390.2793
2178 391.2866 390.2793
2179 391.2867 390.2794
2180 391.2867 390.2794
2181 391.2867 390.2794
2182 391.2867 390.2794
2183 391.2868 390.2795
2184 391.2868 390.2795
2185 391.2870 390.2797
2186 391.2870 390.2797
2187 391.2872 390.2799
2188 391.2872 390.2799
2189 391.2881 390.2808
2190 391.2885 390.2812
2191 393.0906 392.0833
2192 393.1513 392.1440
2193 393.1522 392.1449
2194 393.1538 392.1465
2195 393.1539 392.1466
2196 393.1542 392.1469
2197 393.1545 392.1472
2198 393.1545 392.1472
2199 393.1546 392.1473
2200 393.1546 392.1473
2201 393.1546 392.1473
2202 393.1547 392.1474
2203 393.1548 392.1475
2204 393.1548 392.1475
2205 393.1549 392.1476
2206 393.1908 392.1835
2207 393.1910 392.1837
2208 393.1910 392.1837
2209 393.1910 392.1837
2210 393.1911 392.1838
2211 393.1911 392.1838
2212 393.1911 392.1838
2213 393.1911 392.1838
2214 393.1912 392.1839
2215 393.1912 392.1839
2216 393.1912 392.1839
2217 393.1912 392.1839
2218 393.1912 392.1839
2219 393.1913 392.1840
2220 393.1913 392.1840
2221 393.1913 392.1840
2222 393.1922 392.1849
2223 393.2292 392.2219
2224 393.2458 392.2385
2225 393.2460 392.2387
2226 393.2460 392.2387
2227 393.2461 392.2388
2228 393.2461 392.2388
2229 393.2461 392.2388
2230 393.2462 392.2389
2231 393.2465 392.2392
2232 393.2477 392.2404
2233 393.2990 392.2917
2234 393.2993 392.2920
2235 393.2997 392.2924
2236 393.2997 392.2924
2237 393.2997 392.2924
2238 393.2997 392.2924
2239 393.2997 392.2924
2240 393.2997 392.2924
2241 393.2997 392.2924
2242 393.2998 392.2925
2243 393.2998 392.2925
2244 393.2998 392.2925
2245 393.2998 392.2925
2246 393.2998 392.2925
2247 393.2998 392.2925
2248 393.2999 392.2926
2249 393.2999 392.2926
2250 393.2999 392.2926
2251 393.2999 392.2926
2252 393.2999 392.2926
2253 393.2999 392.2926
2254 393.3000 392.2927
2255 393.3000 392.2927
2256 393.3000 392.2927
2257 393.3000 392.2927
2258 393.3000 392.2927
2259 393.3001 392.2928
2260 393.3002 392.2929
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