MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy1
MS data file : PRT1311_JBEI_1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 8 Sep 2025 at 17:17:19 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,450

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2301–2400 (out of 4217)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2061 383.1301 382.1228
2062 383.1304 382.1231
2063 383.1337 382.1264
2064 383.1690 382.1617
2065 383.1693 382.1620
2066 383.1699 382.1626
2067 383.1699 382.1626
2068 383.1700 382.1627
2069 383.1703 382.1630
2070 383.1704 382.1631
2071 383.1705 382.1632
2072 383.1706 382.1633
2073 383.1707 382.1634
2074 383.1709 382.1636
2075 383.1711 382.1638
2076 383.1715 382.1642
2077 383.2065 382.1992
2078 383.2071 382.1998
2079 383.2073 382.2000
2080 383.2249 382.2176
2081 383.2258 382.2185
2082 383.2261 382.2188
2083 383.2264 382.2191
2084 383.2265 382.2192
2085 383.2268 382.2195
2086 383.2269 382.2196
2087 383.2269 382.2196
2088 383.2271 382.2198
2089 383.2277 382.2204
2090 383.9535 382.9462
2091 385.1067 384.0994
2092 385.1459 384.1386
2093 385.1460 384.1387
2094 385.1465 384.1392
2095 385.1482 384.1409
2096 385.1867 384.1794
2097 385.1870 384.1797
2098 385.1872 384.1799
2099 385.1878 384.1805
2100 385.2214 384.2141
2101 385.2214 384.2141
2102 385.2430 384.2357
2103 385.2431 384.2358
2104 385.2432 384.2359
2105 385.2439 384.2366
2106 385.2443 384.2370
2107 385.9688 384.9615
2108 387.1230 386.1157
2109 387.1232 386.1159
2110 387.1803 386.1730
2111 387.1972 386.1899
2112 387.2005 386.1932
2113 389.1598 388.1525
2114 389.1599 388.1526
2115 389.1600 388.1527
2116 389.1601 388.1528
2117 389.1604 388.1531
2118 389.1964 388.1891
2119 389.1964 388.1891
2120 389.1966 388.1893
2121 389.1971 388.1898
2122 389.2530 388.2457
2123 391.1361 390.1288
2124 391.1384 390.1311
2125 391.1384 390.1311
2126 391.1385 390.1312
2127 391.1386 390.1313
2128 391.1387 390.1314
2129 391.1389 390.1316
2130 391.1394 390.1321
2131 391.1746 390.1673
2132 391.1751 390.1678
2133 391.1751 390.1678
2134 391.1752 390.1679
2135 391.1752 390.1679
2136 391.1753 390.1680
2137 391.1753 390.1680
2138 391.1754 390.1681
2139 391.1755 390.1682
2140 391.1755 390.1682
2141 391.1756 390.1683
2142 391.1758 390.1685
2143 391.1759 390.1686
2144 391.1759 390.1686
2145 391.2117 390.2044
2146 391.2118 390.2045
2147 391.2121 390.2048
2148 391.2122 390.2049
2149 391.2123 390.2050
2150 391.2123 390.2050
2151 391.2124 390.2051
2152 391.2124 390.2051
2153 391.2125 390.2052
2154 391.2125 390.2052
2155 391.2126 390.2053
2156 391.2320 390.2247
2157 391.2858 390.2785
2158 391.2860 390.2787
2159 391.2862 390.2789
2160 391.2863 390.2790
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