MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy1
MS data file : PRT1311_JBEI_1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 8 Sep 2025 at 17:17:19 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,450

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2201–2300 (out of 4217)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1961 379.1356 378.1283
1962 379.1364 378.1291
1963 379.1379 378.1306
1964 379.1381 378.1308
1965 379.1384 378.1311
1966 379.1385 378.1312
1967 379.1385 378.1312
1968 379.1389 378.1316
1969 379.1389 378.1316
1970 379.1389 378.1316
1971 379.1391 378.1318
1972 379.1394 378.1321
1973 379.1749 378.1676
1974 379.1749 378.1676
1975 379.1751 378.1678
1976 379.1751 378.1678
1977 379.1751 378.1678
1978 379.1752 378.1679
1979 379.1753 378.1680
1980 379.1753 378.1680
1981 379.1753 378.1680
1982 379.1753 378.1680
1983 379.1753 378.1680
1984 379.1754 378.1681
1985 379.1754 378.1681
1986 379.1754 378.1681
1987 379.1754 378.1681
1988 379.1754 378.1681
1989 379.1755 378.1682
1990 379.1756 378.1683
1991 379.1757 378.1684
1992 379.1938 378.1865
1993 379.1941 378.1868
1994 379.2112 378.2039
1995 379.2113 378.2040
1996 379.2114 378.2041
1997 379.2114 378.2041
1998 379.2117 378.2044
1999 379.2118 378.2045
2000 379.2119 378.2046
2001 379.2120 378.2047
2002 379.2122 378.2049
2003 379.2122 378.2049
2004 379.2125 378.2052
2005 379.2126 378.2053
2006 379.2682 378.2609
2007 381.0378 380.0305
2008 381.0385 380.0312
2009 381.1129 380.1056
2010 381.1516 380.1443
2011 381.1526 380.1453
2012 381.1536 380.1463
2013 381.1540 380.1467
2014 381.1542 380.1469
2015 381.1543 380.1470
2016 381.1543 380.1470
2017 381.1543 380.1470
2018 381.1543 380.1470
2019 381.1544 380.1471
2020 381.1544 380.1471
2021 381.1545 380.1472
2022 381.1546 380.1473
2023 381.1548 380.1475
2024 381.1550 380.1477
2025 381.1898 380.1825
2026 381.1902 380.1829
2027 381.1905 380.1832
2028 381.1906 380.1833
2029 381.1906 380.1833
2030 381.1906 380.1833
2031 381.1908 380.1835
2032 381.1908 380.1835
2033 381.1909 380.1836
2034 381.1909 380.1836
2035 381.1909 380.1836
2036 381.1910 380.1837
2037 381.1910 380.1837
2038 381.1911 380.1838
2039 381.1911 380.1838
2040 381.1911 380.1838
2041 381.1911 380.1838
2042 381.1911 380.1838
2043 381.1912 380.1839
2044 381.1914 380.1841
2045 381.1915 380.1842
2046 381.2040 380.1967
2047 381.2101 380.2028
2048 381.2102 380.2029
2049 381.2103 380.2030
2050 381.2104 380.2031
2051 381.2104 380.2031
2052 381.2104 380.2031
2053 381.2105 380.2032
2054 381.2105 380.2032
2055 381.2106 380.2033
2056 381.2106 380.2033
2057 381.2106 380.2033
2058 381.2112 380.2039
2059 383.1030 382.0957
2060 383.1292 382.1219
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