MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy1
MS data file : PRT1311_JBEI_1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 8 Sep 2025 at 17:17:19 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,450

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2101–2200 (out of 4217)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1861 371.2656 370.2583
1862 371.3168 370.3095
1863 371.3170 370.3097
1864 371.3173 370.3100
1865 371.3174 370.3101
1866 371.3174 370.3101
1867 371.3174 370.3101
1868 371.3174 370.3101
1869 371.3174 370.3101
1870 371.3174 370.3101
1871 371.3174 370.3101
1872 371.3174 370.3101
1873 371.3174 370.3101
1874 371.3174 370.3101
1875 371.3175 370.3102
1876 371.3175 370.3102
1877 371.3175 370.3102
1878 371.3175 370.3102
1879 371.3175 370.3102
1880 371.3175 370.3102
1881 371.3175 370.3102
1882 371.3176 370.3103
1883 371.3176 370.3103
1884 371.3176 370.3103
1885 371.3176 370.3103
1886 371.3177 370.3104
1887 371.3177 370.3104
1888 371.3177 370.3104
1889 371.3178 370.3105
1890 371.3180 370.3107
1891 371.3180 370.3107
1892 371.3181 370.3108
1893 371.3181 370.3108
1894 371.3183 370.3110
1895 371.3191 370.3118
1896 371.3194 370.3121
1897 372.0523 371.0450
1898 372.0527 371.0454
1899 372.9984 371.9911
1900 373.0933 372.0860
1901 373.0941 372.0868
1902 373.0943 372.0870
1903 373.1091 372.1018
1904 373.1653 372.1580
1905 373.9995 372.9922
1906 373.9998 372.9925
1907 374.9932 373.9859
1908 375.0674 374.0601
1909 375.0674 374.0601
1910 375.0674 374.0601
1911 375.0675 374.0602
1912 375.0677 374.0604
1913 375.1428 374.1355
1914 375.1434 374.1361
1915 375.1436 374.1363
1916 375.1436 374.1363
1917 375.1436 374.1363
1918 375.1439 374.1366
1919 375.1439 374.1366
1920 375.1439 374.1366
1921 375.1442 374.1369
1922 375.1801 374.1728
1923 375.1804 374.1731
1924 375.1804 374.1731
1925 375.1806 374.1733
1926 375.8635 374.8562
1927 375.8635 374.8562
1928 375.9956 374.9883
1929 377.0072 375.9999
1930 377.1569 376.1496
1931 377.1590 376.1517
1932 377.1592 376.1519
1933 377.1592 376.1519
1934 377.1593 376.1520
1935 377.1594 376.1521
1936 377.1594 376.1521
1937 377.1595 376.1522
1938 377.1595 376.1522
1939 377.1595 376.1522
1940 377.1595 376.1522
1941 377.1595 376.1522
1942 377.1596 376.1523
1943 377.1596 376.1523
1944 377.1599 376.1526
1945 377.1600 376.1527
1946 377.1601 376.1528
1947 377.1957 376.1884
1948 377.1960 376.1887
1949 377.1960 376.1887
1950 377.1961 376.1888
1951 377.1962 376.1889
1952 377.1962 376.1889
1953 377.1963 376.1890
1954 377.1964 376.1891
1955 377.1964 376.1891
1956 377.1964 376.1891
1957 377.1964 376.1891
1958 377.1966 376.1893
1959 377.1967 376.1894
1960 377.9911 376.9838
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