MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy1
MS data file : PRT1311_JBEI_1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 8 Sep 2025 at 17:17:19 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,450

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 2001–2100 (out of 4217)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1761 367.1749 366.1676
1762 367.1749 366.1676
1763 367.1750 366.1677
1764 367.1750 366.1677
1765 367.1750 366.1677
1766 367.1752 366.1679
1767 367.1752 366.1679
1768 367.1752 366.1679
1769 367.1752 366.1679
1770 367.1753 366.1680
1771 367.1753 366.1680
1772 367.1753 366.1680
1773 367.1753 366.1680
1774 367.1754 366.1681
1775 367.1754 366.1681
1776 367.1754 366.1681
1777 367.1754 366.1681
1778 367.1756 366.1683
1779 367.1758 366.1685
1780 367.1929 366.1856
1781 367.1933 366.1860
1782 367.1934 366.1861
1783 367.1938 366.1865
1784 367.1938 366.1865
1785 367.1939 366.1866
1786 367.1942 366.1869
1787 367.2111 366.2038
1788 367.2118 366.2045
1789 367.2122 366.2049
1790 367.2122 366.2049
1791 367.2122 366.2049
1792 367.2318 366.2245
1793 367.2319 366.2246
1794 367.2320 366.2247
1795 367.2320 366.2247
1796 367.2321 366.2248
1797 367.2321 366.2248
1798 367.2322 366.2249
1799 367.2323 366.2250
1800 367.2323 366.2250
1801 367.2324 366.2251
1802 367.2324 366.2251
1803 367.2325 366.2252
1804 367.2332 366.2259
1805 367.2336 366.2263
1806 368.0588 367.0515
1807 369.1131 368.1058
1808 369.1133 368.1060
1809 369.1142 368.1069
1810 369.1161 368.1088
1811 369.1532 368.1459
1812 369.1534 368.1461
1813 369.1535 368.1462
1814 369.1541 368.1468
1815 369.1542 368.1469
1816 369.1543 368.1470
1817 369.1547 368.1474
1818 369.1548 368.1475
1819 369.1548 368.1475
1820 369.1550 368.1477
1821 369.1552 368.1479
1822 369.1554 368.1481
1823 369.1714 368.1641
1824 369.1717 368.1644
1825 369.1718 368.1645
1826 369.1719 368.1646
1827 369.1721 368.1648
1828 369.1912 368.1839
1829 369.1913 368.1840
1830 369.2103 368.2030
1831 369.2104 368.2031
1832 369.2484 368.2411
1833 370.0287 369.0214
1834 370.0290 369.0217
1835 370.0290 369.0217
1836 370.0290 369.0217
1837 370.2510 369.2437
1838 370.2513 369.2440
1839 370.2519 369.2446
1840 370.8844 369.8771
1841 370.8844 369.8771
1842 370.8847 369.8774
1843 370.8849 369.8776
1844 370.8849 369.8776
1845 370.8849 369.8776
1846 370.8849 369.8776
1847 370.8849 369.8776
1848 370.8850 369.8777
1849 370.8851 369.8778
1850 370.9995 369.9922
1851 371.0797 370.0724
1852 371.0797 370.0724
1853 371.1046 370.0973
1854 371.1049 370.0976
1855 371.1053 370.0980
1856 371.1284 370.1211
1857 371.1291 370.1218
1858 371.1294 370.1221
1859 371.1325 370.1252
1860 371.1717 370.1644
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