MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy1
MS data file : PRT1311_JBEI_1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 8 Sep 2025 at 17:17:19 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,450

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1901–2000 (out of 4217)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1661 363.1800 362.1727
1662 363.1801 362.1728
1663 363.1801 362.1728
1664 363.1802 362.1729
1665 363.1802 362.1729
1666 363.1802 362.1729
1667 363.1802 362.1729
1668 363.1802 362.1729
1669 363.1803 362.1730
1670 363.1803 362.1730
1671 363.1803 362.1730
1672 363.1803 362.1730
1673 363.1804 362.1731
1674 363.1804 362.1731
1675 363.1804 362.1731
1676 363.1804 362.1731
1677 363.1806 362.1733
1678 363.1807 362.1734
1679 363.1807 362.1734
1680 363.1809 362.1736
1681 363.2175 362.2102
1682 364.9938 363.9865
1683 364.9940 363.9867
1684 364.9942 363.9869
1685 365.1195 364.1122
1686 365.1203 364.1130
1687 365.1205 364.1132
1688 365.1218 364.1145
1689 365.1227 364.1154
1690 365.1229 364.1156
1691 365.1230 364.1157
1692 365.1585 364.1512
1693 365.1588 364.1515
1694 365.1592 364.1519
1695 365.1593 364.1520
1696 365.1593 364.1520
1697 365.1594 364.1521
1698 365.1594 364.1521
1699 365.1594 364.1521
1700 365.1595 364.1522
1701 365.1595 364.1522
1702 365.1595 364.1522
1703 365.1595 364.1522
1704 365.1596 364.1523
1705 365.1596 364.1523
1706 365.1596 364.1523
1707 365.1597 364.1524
1708 365.1597 364.1524
1709 365.1598 364.1525
1710 365.1957 364.1884
1711 365.1958 364.1885
1712 365.1958 364.1885
1713 365.1958 364.1885
1714 365.1958 364.1885
1715 365.1958 364.1885
1716 365.1958 364.1885
1717 365.1958 364.1885
1718 365.1959 364.1886
1719 365.1959 364.1886
1720 365.1960 364.1887
1721 365.1960 364.1887
1722 365.1961 364.1888
1723 365.1961 364.1888
1724 365.1961 364.1888
1725 365.1962 364.1889
1726 365.1963 364.1890
1727 365.1963 364.1890
1728 365.1963 364.1890
1729 365.1964 364.1891
1730 365.1969 364.1896
1731 365.1980 364.1907
1732 365.2152 364.2079
1733 365.2156 364.2083
1734 365.2156 364.2083
1735 365.2156 364.2083
1736 365.2157 364.2084
1737 365.2158 364.2085
1738 365.2158 364.2085
1739 365.2158 364.2085
1740 365.2158 364.2085
1741 365.2159 364.2086
1742 365.2159 364.2086
1743 365.2161 364.2088
1744 365.2162 364.2089
1745 365.2166 364.2093
1746 366.9933 365.9860
1747 367.1357 366.1284
1748 367.1365 366.1292
1749 367.1365 366.1292
1750 367.1376 366.1303
1751 367.1380 366.1307
1752 367.1385 366.1312
1753 367.1385 366.1312
1754 367.1385 366.1312
1755 367.1386 366.1313
1756 367.1386 366.1313
1757 367.1386 366.1313
1758 367.1387 366.1314
1759 367.1387 366.1314
1760 367.1747 366.1674
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