MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy1
MS data file : PRT1311_JBEI_1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 8 Sep 2025 at 17:17:19 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,450

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1801–1900 (out of 4217)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1561 355.1928 354.1855
1562 355.1931 354.1858
1563 355.1935 354.1862
1564 355.1942 354.1869
1565 355.2688 354.2615
1566 355.2690 354.2617
1567 355.9581 354.9508
1568 355.9886 354.9813
1569 355.9888 354.9815
1570 356.1322 355.1249
1571 356.1324 355.1251
1572 356.2719 355.2646
1573 356.2732 355.2659
1574 357.1120 356.1047
1575 357.1132 356.1059
1576 357.1147 356.1074
1577 357.1151 356.1078
1578 357.1523 356.1450
1579 357.1526 356.1453
1580 357.1917 356.1844
1581 357.9539 356.9466
1582 357.9738 356.9665
1583 357.9741 356.9668
1584 359.0929 358.0856
1585 359.1111 358.1038
1586 359.1111 358.1038
1587 359.1117 358.1044
1588 359.1118 358.1045
1589 359.1118 358.1045
1590 359.1119 358.1046
1591 359.1122 358.1049
1592 359.1132 358.1059
1593 359.1483 358.1410
1594 359.1484 358.1411
1595 359.1486 358.1413
1596 359.1487 358.1414
1597 359.1488 358.1415
1598 359.1489 358.1416
1599 359.1490 358.1417
1600 359.1490 358.1417
1601 359.1490 358.1417
1602 359.1491 358.1418
1603 359.1493 358.1420
1604 359.1724 358.1651
1605 359.2421 358.2348
1606 359.2424 358.2351
1607 359.9695 358.9622
1608 359.9699 358.9626
1609 361.0505 360.0432
1610 361.0515 360.0442
1611 361.0518 360.0445
1612 361.1270 360.1197
1613 361.1270 360.1197
1614 361.1271 360.1198
1615 361.1272 360.1199
1616 361.1272 360.1199
1617 361.1277 360.1204
1618 361.1642 360.1569
1619 361.1643 360.1570
1620 361.1643 360.1570
1621 361.1644 360.1571
1622 361.1645 360.1572
1623 361.1645 360.1572
1624 361.1645 360.1572
1625 361.1646 360.1573
1626 361.1646 360.1573
1627 361.1646 360.1573
1628 361.1647 360.1574
1629 361.1647 360.1574
1630 361.1647 360.1574
1631 361.1647 360.1574
1632 361.1651 360.1578
1633 361.1808 360.1735
1634 361.2004 360.1931
1635 361.2008 360.1935
1636 361.2011 360.1938
1637 361.2018 360.1945
1638 362.0416 361.0343
1639 362.9994 361.9921
1640 363.0260 362.0187
1641 363.1040 362.0967
1642 363.1042 362.0969
1643 363.1405 362.1332
1644 363.1424 362.1351
1645 363.1429 362.1356
1646 363.1434 362.1361
1647 363.1434 362.1361
1648 363.1435 362.1362
1649 363.1436 362.1363
1650 363.1436 362.1363
1651 363.1436 362.1363
1652 363.1436 362.1363
1653 363.1437 362.1364
1654 363.1437 362.1364
1655 363.1437 362.1364
1656 363.1439 362.1366
1657 363.1440 362.1367
1658 363.1441 362.1368
1659 363.1800 362.1727
1660 363.1800 362.1727
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