MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy1
MS data file : PRT1311_JBEI_1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 8 Sep 2025 at 17:17:19 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,450

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1701–1800 (out of 4217)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1461 353.1216 352.1143
1462 353.1218 352.1145
1463 353.1223 352.1150
1464 353.1223 352.1150
1465 353.1227 352.1154
1466 353.1228 352.1155
1467 353.1228 352.1155
1468 353.1588 352.1515
1469 353.1589 352.1516
1470 353.1591 352.1518
1471 353.1591 352.1518
1472 353.1591 352.1518
1473 353.1591 352.1518
1474 353.1592 352.1519
1475 353.1596 352.1523
1476 353.1596 352.1523
1477 353.1596 352.1523
1478 353.1597 352.1524
1479 353.1598 352.1525
1480 353.1598 352.1525
1481 353.1598 352.1525
1482 353.1599 352.1526
1483 353.1599 352.1526
1484 353.1600 352.1527
1485 353.1772 352.1699
1486 353.1772 352.1699
1487 353.1772 352.1699
1488 353.1776 352.1703
1489 353.1776 352.1703
1490 353.1954 352.1881
1491 353.1962 352.1889
1492 353.1962 352.1889
1493 353.1963 352.1890
1494 353.1965 352.1892
1495 353.2136 352.2063
1496 353.2143 352.2070
1497 353.2154 352.2081
1498 353.2158 352.2085
1499 353.2324 352.2251
1500 353.2326 352.2253
1501 353.2529 352.2456
1502 353.2530 352.2457
1503 353.2530 352.2457
1504 353.2530 352.2457
1505 353.2533 352.2460
1506 353.2534 352.2461
1507 353.2542 352.2469
1508 354.2174 353.2101
1509 354.2563 353.2490
1510 355.0385 354.0312
1511 355.0386 354.0313
1512 355.0390 354.0317
1513 355.0706 354.0633
1514 355.0707 354.0634
1515 355.0715 354.0642
1516 355.0716 354.0643
1517 355.0719 354.0646
1518 355.0720 354.0647
1519 355.0721 354.0648
1520 355.0721 354.0648
1521 355.0721 354.0648
1522 355.0721 354.0648
1523 355.0722 354.0649
1524 355.0722 354.0649
1525 355.0722 354.0649
1526 355.0722 354.0649
1527 355.0723 354.0650
1528 355.0724 354.0651
1529 355.0724 354.0651
1530 355.0725 354.0652
1531 355.0726 354.0653
1532 355.0728 354.0655
1533 355.0731 354.0658
1534 355.0829 354.0756
1535 355.0831 354.0758
1536 355.0832 354.0759
1537 355.0985 354.0912
1538 355.1081 354.1008
1539 355.1360 354.1287
1540 355.1362 354.1289
1541 355.1368 354.1295
1542 355.1383 354.1310
1543 355.1385 354.1312
1544 355.1387 354.1314
1545 355.1389 354.1316
1546 355.1391 354.1318
1547 355.1392 354.1319
1548 355.1649 354.1576
1549 355.1747 354.1674
1550 355.1751 354.1678
1551 355.1752 354.1679
1552 355.1761 354.1688
1553 355.1762 354.1689
1554 355.1762 354.1689
1555 355.1764 354.1691
1556 355.1765 354.1692
1557 355.1765 354.1692
1558 355.1766 354.1693
1559 355.1924 354.1851
1560 355.1927 354.1854
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