| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Andy1 |
| MS data file | : | PRT1311_JBEI_1.mgf |
| Database | : | Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues) |
| Timestamp | : | 8 Sep 2025 at 17:17:19 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | |
| Variable modifications | : | |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,450 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 | 2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | ||||
| 6.2e-007 | 1 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | ||||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle (
) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.
| Query | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | Peptide |
|---|---|---|---|---|---|---|---|---|---|
| Query | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | Peptide |
| 353.1216 | 352.1143 | ||||||||
| 353.1218 | 352.1145 | ||||||||
| 353.1223 | 352.1150 | ||||||||
| 353.1223 | 352.1150 | ||||||||
| 353.1227 | 352.1154 | ||||||||
| 353.1228 | 352.1155 | ||||||||
| 353.1228 | 352.1155 | ||||||||
| 353.1588 | 352.1515 | ||||||||
| 353.1589 | 352.1516 | ||||||||
| 353.1591 | 352.1518 | ||||||||
| 353.1591 | 352.1518 | ||||||||
| 353.1591 | 352.1518 | ||||||||
| 353.1591 | 352.1518 | ||||||||
| 353.1592 | 352.1519 | ||||||||
| 353.1596 | 352.1523 | ||||||||
| 353.1596 | 352.1523 | ||||||||
| 353.1596 | 352.1523 | ||||||||
| 353.1597 | 352.1524 | ||||||||
| 353.1598 | 352.1525 | ||||||||
| 353.1598 | 352.1525 | ||||||||
| 353.1598 | 352.1525 | ||||||||
| 353.1599 | 352.1526 | ||||||||
| 353.1599 | 352.1526 | ||||||||
| 353.1600 | 352.1527 | ||||||||
| 353.1772 | 352.1699 | ||||||||
| 353.1772 | 352.1699 | ||||||||
| 353.1772 | 352.1699 | ||||||||
| 353.1776 | 352.1703 | ||||||||
| 353.1776 | 352.1703 | ||||||||
| 353.1954 | 352.1881 | ||||||||
| 353.1962 | 352.1889 | ||||||||
| 353.1962 | 352.1889 | ||||||||
| 353.1963 | 352.1890 | ||||||||
| 353.1965 | 352.1892 | ||||||||
| 353.2136 | 352.2063 | ||||||||
| 353.2143 | 352.2070 | ||||||||
| 353.2154 | 352.2081 | ||||||||
| 353.2158 | 352.2085 | ||||||||
| 353.2324 | 352.2251 | ||||||||
| 353.2326 | 352.2253 | ||||||||
| 353.2529 | 352.2456 | ||||||||
| 353.2530 | 352.2457 | ||||||||
| 353.2530 | 352.2457 | ||||||||
| 353.2530 | 352.2457 | ||||||||
| 353.2533 | 352.2460 | ||||||||
| 353.2534 | 352.2461 | ||||||||
| 353.2542 | 352.2469 | ||||||||
| 354.2174 | 353.2101 | ||||||||
| 354.2563 | 353.2490 | ||||||||
| 355.0385 | 354.0312 | ||||||||
| 355.0386 | 354.0313 | ||||||||
| 355.0390 | 354.0317 | ||||||||
| 355.0706 | 354.0633 | ||||||||
| 355.0707 | 354.0634 | ||||||||
| 355.0715 | 354.0642 | ||||||||
| 355.0716 | 354.0643 | ||||||||
| 355.0719 | 354.0646 | ||||||||
| 355.0720 | 354.0647 | ||||||||
| 355.0721 | 354.0648 | ||||||||
| 355.0721 | 354.0648 | ||||||||
| 355.0721 | 354.0648 | ||||||||
| 355.0721 | 354.0648 | ||||||||
| 355.0722 | 354.0649 | ||||||||
| 355.0722 | 354.0649 | ||||||||
| 355.0722 | 354.0649 | ||||||||
| 355.0722 | 354.0649 | ||||||||
| 355.0723 | 354.0650 | ||||||||
| 355.0724 | 354.0651 | ||||||||
| 355.0724 | 354.0651 | ||||||||
| 355.0725 | 354.0652 | ||||||||
| 355.0726 | 354.0653 | ||||||||
| 355.0728 | 354.0655 | ||||||||
| 355.0731 | 354.0658 | ||||||||
| 355.0829 | 354.0756 | ||||||||
| 355.0831 | 354.0758 | ||||||||
| 355.0832 | 354.0759 | ||||||||
| 355.0985 | 354.0912 | ||||||||
| 355.1081 | 354.1008 | ||||||||
| 355.1360 | 354.1287 | ||||||||
| 355.1362 | 354.1289 | ||||||||
| 355.1368 | 354.1295 | ||||||||
| 355.1383 | 354.1310 | ||||||||
| 355.1385 | 354.1312 | ||||||||
| 355.1387 | 354.1314 | ||||||||
| 355.1389 | 354.1316 | ||||||||
| 355.1391 | 354.1318 | ||||||||
| 355.1392 | 354.1319 | ||||||||
| 355.1649 | 354.1576 | ||||||||
| 355.1747 | 354.1674 | ||||||||
| 355.1751 | 354.1678 | ||||||||
| 355.1752 | 354.1679 | ||||||||
| 355.1761 | 354.1688 | ||||||||
| 355.1762 | 354.1689 | ||||||||
| 355.1762 | 354.1689 | ||||||||
| 355.1764 | 354.1691 | ||||||||
| 355.1765 | 354.1692 | ||||||||
| 355.1765 | 354.1692 | ||||||||
| 355.1766 | 354.1693 | ||||||||
| 355.1924 | 354.1851 | ||||||||
| 355.1927 | 354.1854 | ||||||||
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