MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy1
MS data file : PRT1311_JBEI_1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 8 Sep 2025 at 17:17:19 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,450

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1601–1700 (out of 4217)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1361 349.2212 348.2139
1362 349.2213 348.2140
1363 349.2214 348.2141
1364 349.2214 348.2141
1365 349.2215 348.2142
1366 349.2216 348.2143
1367 349.2216 348.2143
1368 349.2216 348.2143
1369 349.2220 348.2147
1370 349.2220 348.2147
1371 349.2227 348.2154
1372 349.2227 348.2154
1373 351.0496 350.0423
1374 351.1034 350.0961
1375 351.1034 350.0961
1376 351.1045 350.0972
1377 351.1052 350.0979
1378 351.1417 350.1344
1379 351.1430 350.1357
1380 351.1432 350.1359
1381 351.1433 350.1360
1382 351.1434 350.1361
1383 351.1435 350.1362
1384 351.1435 350.1362
1385 351.1436 350.1363
1386 351.1436 350.1363
1387 351.1436 350.1363
1388 351.1437 350.1364
1389 351.1438 350.1365
1390 351.1439 350.1366
1391 351.1440 350.1367
1392 351.1799 350.1726
1393 351.1799 350.1726
1394 351.1799 350.1726
1395 351.1799 350.1726
1396 351.1799 350.1726
1397 351.1800 350.1727
1398 351.1800 350.1727
1399 351.1800 350.1727
1400 351.1800 350.1727
1401 351.1800 350.1727
1402 351.1800 350.1727
1403 351.1801 350.1728
1404 351.1801 350.1728
1405 351.1801 350.1728
1406 351.1801 350.1728
1407 351.1801 350.1728
1408 351.1802 350.1729
1409 351.1803 350.1730
1410 351.1985 350.1912
1411 351.1985 350.1912
1412 351.1989 350.1916
1413 351.1998 350.1925
1414 351.2163 350.2090
1415 351.2163 350.2090
1416 351.2164 350.2091
1417 351.2164 350.2091
1418 351.2165 350.2092
1419 351.2165 350.2092
1420 351.2166 350.2093
1421 351.2166 350.2093
1422 351.2167 350.2094
1423 351.2167 350.2094
1424 351.2168 350.2095
1425 351.2168 350.2095
1426 351.2168 350.2095
1427 351.2170 350.2097
1428 351.2177 350.2104
1429 351.2365 350.2292
1430 351.2368 350.2295
1431 351.2369 350.2296
1432 351.2371 350.2298
1433 351.2371 350.2298
1434 351.2371 350.2298
1435 351.2371 350.2298
1436 351.2372 350.2299
1437 351.2373 350.2300
1438 351.2374 350.2301
1439 351.2374 350.2301
1440 351.2376 350.2303
1441 351.2377 350.2304
1442 351.2383 350.2310
1443 352.0119 351.0046
1444 352.0120 351.0047
1445 352.0120 351.0047
1446 352.0121 351.0048
1447 352.0121 351.0048
1448 352.2198 351.2125
1449 352.2201 351.2128
1450 352.2202 351.2129
1451 352.2205 351.2132
1452 353.0311 352.0238
1453 353.0429 352.0356
1454 353.0435 352.0362
1455 353.0699 352.0626
1456 353.0849 352.0776
1457 353.0849 352.0776
1458 353.0852 352.0779
1459 353.1200 352.1127
1460 353.1208 352.1135
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