MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy1
MS data file : PRT1311_JBEI_1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 8 Sep 2025 at 17:17:19 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,450

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1501–1600 (out of 4217)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1261 347.1485 346.1412
1262 347.1486 346.1413
1263 347.1486 346.1413
1264 347.1486 346.1413
1265 347.1486 346.1413
1266 347.1486 346.1413
1267 347.1487 346.1414
1268 347.1487 346.1414
1269 347.1487 346.1414
1270 347.1488 346.1415
1271 347.1489 346.1416
1272 347.1849 346.1776
1273 347.1850 346.1777
1274 347.1851 346.1778
1275 347.1851 346.1778
1276 347.1852 346.1779
1277 347.1852 346.1779
1278 347.1852 346.1779
1279 347.1852 346.1779
1280 347.1853 346.1780
1281 347.1853 346.1780
1282 347.1853 346.1780
1283 347.1853 346.1780
1284 347.1854 346.1781
1285 347.1854 346.1781
1286 347.1856 346.1783
1287 347.1856 346.1783
1288 347.1857 346.1784
1289 347.1858 346.1785
1290 347.1868 346.1795
1291 348.0528 347.0455
1292 348.0528 347.0455
1293 348.0533 347.0460
1294 348.2033 347.1960
1295 348.2034 347.1961
1296 348.2035 347.1962
1297 348.2036 347.1963
1298 349.0300 348.0227
1299 349.0309 348.0236
1300 349.0311 348.0238
1301 349.0362 348.0289
1302 349.1247 348.1174
1303 349.1258 348.1185
1304 349.1263 348.1190
1305 349.1272 348.1199
1306 349.1272 348.1199
1307 349.1274 348.1201
1308 349.1277 348.1204
1309 349.1277 348.1204
1310 349.1277 348.1204
1311 349.1277 348.1204
1312 349.1278 348.1205
1313 349.1278 348.1205
1314 349.1279 348.1206
1315 349.1280 348.1207
1316 349.1281 348.1208
1317 349.1642 348.1569
1318 349.1642 348.1569
1319 349.1642 348.1569
1320 349.1642 348.1569
1321 349.1643 348.1570
1322 349.1643 348.1570
1323 349.1643 348.1570
1324 349.1643 348.1570
1325 349.1643 348.1570
1326 349.1643 348.1570
1327 349.1643 348.1570
1328 349.1644 348.1571
1329 349.1644 348.1571
1330 349.1644 348.1571
1331 349.1645 348.1572
1332 349.1645 348.1572
1333 349.1645 348.1572
1334 349.1646 348.1573
1335 349.1647 348.1574
1336 349.1648 348.1575
1337 349.1650 348.1577
1338 349.1660 348.1587
1339 349.1855 348.1782
1340 349.2005 348.1932
1341 349.2006 348.1933
1342 349.2006 348.1933
1343 349.2006 348.1933
1344 349.2007 348.1934
1345 349.2007 348.1934
1346 349.2008 348.1935
1347 349.2008 348.1935
1348 349.2008 348.1935
1349 349.2008 348.1935
1350 349.2008 348.1935
1351 349.2009 348.1936
1352 349.2010 348.1937
1353 349.2011 348.1938
1354 349.2011 348.1938
1355 349.2011 348.1938
1356 349.2012 348.1939
1357 349.2013 348.1940
1358 349.2023 348.1950
1359 349.2210 348.2137
1360 349.2210 348.2137
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