MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy1
MS data file : PRT1311_JBEI_1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 8 Sep 2025 at 17:17:19 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,450

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1401–1500 (out of 4217)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1161 341.1583 340.1510
1162 341.1586 340.1513
1163 341.1591 340.1518
1164 341.1592 340.1519
1165 341.1593 340.1520
1166 341.1593 340.1520
1167 341.1593 340.1520
1168 341.1594 340.1521
1169 341.1594 340.1521
1170 341.1594 340.1521
1171 341.1594 340.1521
1172 341.1595 340.1522
1173 341.1599 340.1526
1174 341.1603 340.1530
1175 341.1604 340.1531
1176 341.1955 340.1882
1177 341.1964 340.1891
1178 341.1964 340.1891
1179 341.1968 340.1895
1180 341.1969 340.1896
1181 341.1970 340.1897
1182 341.1970 340.1897
1183 341.1971 340.1898
1184 341.1971 340.1898
1185 341.1971 340.1898
1186 341.1972 340.1899
1187 341.1972 340.1899
1188 341.1973 340.1900
1189 341.1974 340.1901
1190 341.1976 340.1903
1191 341.2164 340.2091
1192 341.2166 340.2093
1193 341.2169 340.2096
1194 341.2172 340.2099
1195 341.2533 340.2460
1196 343.0356 342.0283
1197 343.0973 342.0900
1198 343.0974 342.0901
1199 343.0975 342.0902
1200 343.0978 342.0905
1201 343.0978 342.0905
1202 343.0980 342.0907
1203 343.0980 342.0907
1204 343.1157 342.1084
1205 343.1161 342.1088
1206 343.1163 342.1090
1207 343.1163 342.1090
1208 343.1165 342.1092
1209 343.1170 342.1097
1210 343.1346 342.1273
1211 343.1351 342.1278
1212 343.1414 342.1341
1213 343.1544 342.1471
1214 343.1545 342.1472
1215 343.2334 342.2261
1216 344.9173 343.9100
1217 345.1128 344.1055
1218 345.1145 344.1072
1219 345.1145 344.1072
1220 345.1320 344.1247
1221 345.1323 344.1250
1222 345.1324 344.1251
1223 345.1327 344.1254
1224 345.1327 344.1254
1225 345.1327 344.1254
1226 345.1328 344.1255
1227 345.1328 344.1255
1228 345.1329 344.1256
1229 345.1330 344.1257
1230 345.1331 344.1258
1231 345.1331 344.1258
1232 345.1332 344.1259
1233 345.1693 344.1620
1234 345.1693 344.1620
1235 345.1694 344.1621
1236 345.1696 344.1623
1237 345.1696 344.1623
1238 345.1697 344.1624
1239 345.1698 344.1625
1240 345.1698 344.1625
1241 345.1699 344.1626
1242 345.1701 344.1628
1243 346.0369 345.0296
1244 346.0370 345.0297
1245 346.1874 345.1801
1246 346.9010 345.8937
1247 347.0219 346.0146
1248 347.1100 346.1027
1249 347.1103 346.1030
1250 347.1108 346.1035
1251 347.1109 346.1036
1252 347.1110 346.1037
1253 347.1112 346.1039
1254 347.1116 346.1043
1255 347.1483 346.1410
1256 347.1483 346.1410
1257 347.1483 346.1410
1258 347.1484 346.1411
1259 347.1484 346.1411
1260 347.1485 346.1412
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