MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy1
MS data file : PRT1311_JBEI_1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 8 Sep 2025 at 17:17:19 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,450

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1301–1400 (out of 4217)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1061 337.1643 336.1570
1062 337.1644 336.1571
1063 337.1645 336.1572
1064 337.1645 336.1572
1065 337.1645 336.1572
1066 337.1646 336.1573
1067 337.1646 336.1573
1068 337.1647 336.1574
1069 337.1647 336.1574
1070 337.1647 336.1574
1071 337.1647 336.1574
1072 337.1649 336.1576
1073 337.1824 336.1751
1074 337.1825 336.1752
1075 337.1826 336.1753
1076 337.1826 336.1753
1077 337.1827 336.1754
1078 337.1827 336.1754
1079 337.1828 336.1755
1080 337.1828 336.1755
1081 337.1830 336.1757
1082 337.1833 336.1760
1083 337.2011 336.1938
1084 337.2011 336.1938
1085 337.2012 336.1939
1086 337.2385 336.2312
1087 337.2578 336.2505
1088 337.2579 336.2506
1089 337.2582 336.2509
1090 337.2582 336.2509
1091 337.2589 336.2516
1092 338.0564 337.0491
1093 338.0566 337.0493
1094 338.1660 337.1587
1095 338.1661 337.1588
1096 338.1661 337.1588
1097 338.1661 337.1588
1098 338.1665 337.1592
1099 339.0685 338.0612
1100 339.0694 338.0621
1101 339.1032 338.0959
1102 339.1035 338.0962
1103 339.1040 338.0967
1104 339.1044 338.0971
1105 339.1062 338.0989
1106 339.1069 338.0996
1107 339.1080 338.1007
1108 339.1422 338.1349
1109 339.1425 338.1352
1110 339.1428 338.1355
1111 339.1432 338.1359
1112 339.1434 338.1361
1113 339.1434 338.1361
1114 339.1435 338.1362
1115 339.1436 338.1363
1116 339.1436 338.1363
1117 339.1436 338.1363
1118 339.1436 338.1363
1119 339.1436 338.1363
1120 339.1437 338.1364
1121 339.1438 338.1365
1122 339.1439 338.1366
1123 339.1440 338.1367
1124 339.1798 338.1725
1125 339.1799 338.1726
1126 339.1800 338.1727
1127 339.1800 338.1727
1128 339.1801 338.1728
1129 339.1803 338.1730
1130 339.1808 338.1735
1131 339.1810 338.1737
1132 339.1811 338.1738
1133 339.1989 338.1916
1134 339.1989 338.1916
1135 339.1990 338.1917
1136 339.1991 338.1918
1137 339.1991 338.1918
1138 339.1991 338.1918
1139 339.1992 338.1919
1140 339.1993 338.1920
1141 339.1994 338.1921
1142 339.1994 338.1921
1143 339.1996 338.1923
1144 339.1997 338.1924
1145 339.2738 338.2665
1146 339.2740 338.2667
1147 340.2773 339.2700
1148 341.0227 340.0154
1149 341.1187 340.1114
1150 341.1190 340.1117
1151 341.1202 340.1129
1152 341.1209 340.1136
1153 341.1215 340.1142
1154 341.1379 340.1306
1155 341.1381 340.1308
1156 341.1381 340.1308
1157 341.1385 340.1312
1158 341.1478 340.1405
1159 341.1479 340.1406
1160 341.1480 340.1407
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