MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy1
MS data file : PRT1311_JBEI_1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 8 Sep 2025 at 17:17:19 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,450

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1201–1300 (out of 4217)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
961 335.1108 334.1035
962 335.1112 334.1039
963 335.1113 334.1040
964 335.1114 334.1041
965 335.1115 334.1042
966 335.1117 334.1044
967 335.1481 334.1408
968 335.1484 334.1411
969 335.1484 334.1411
970 335.1485 334.1412
971 335.1485 334.1412
972 335.1485 334.1412
973 335.1485 334.1412
974 335.1486 334.1413
975 335.1486 334.1413
976 335.1487 334.1414
977 335.1487 334.1414
978 335.1487 334.1414
979 335.1487 334.1414
980 335.1487 334.1414
981 335.1487 334.1414
982 335.1487 334.1414
983 335.1488 334.1415
984 335.1488 334.1415
985 335.1490 334.1417
986 335.1490 334.1417
987 335.1849 334.1776
988 335.1849 334.1776
989 335.1850 334.1777
990 335.1850 334.1777
991 335.1850 334.1777
992 335.1851 334.1778
993 335.1851 334.1778
994 335.1851 334.1778
995 335.1851 334.1778
996 335.1852 334.1779
997 335.1852 334.1779
998 335.1852 334.1779
999 335.1852 334.1779
1000 335.1852 334.1779
1001 335.1854 334.1781
1002 335.1854 334.1781
1003 335.1856 334.1783
1004 335.1856 334.1783
1005 335.1858 334.1785
1006 335.1866 334.1793
1007 335.2208 334.2135
1008 335.2212 334.2139
1009 335.2213 334.2140
1010 335.2213 334.2140
1011 335.2213 334.2140
1012 335.2214 334.2141
1013 335.2214 334.2141
1014 335.2215 334.2142
1015 335.2215 334.2142
1016 335.2216 334.2143
1017 335.2216 334.2143
1018 335.2218 334.2145
1019 335.2222 334.2149
1020 335.2228 334.2155
1021 335.2409 334.2336
1022 335.2413 334.2340
1023 335.2414 334.2341
1024 335.2416 334.2343
1025 335.2416 334.2343
1026 335.2419 334.2346
1027 335.2419 334.2346
1028 335.2425 334.2352
1029 335.2433 334.2360
1030 335.9674 334.9601
1031 335.9675 334.9602
1032 335.9676 334.9603
1033 335.9678 334.9605
1034 335.9799 334.9726
1035 336.0160 335.0087
1036 337.0887 336.0814
1037 337.0892 336.0819
1038 337.1252 336.1179
1039 337.1270 336.1197
1040 337.1274 336.1201
1041 337.1276 336.1203
1042 337.1276 336.1203
1043 337.1276 336.1203
1044 337.1279 336.1206
1045 337.1280 336.1207
1046 337.1281 336.1208
1047 337.1284 336.1211
1048 337.1287 336.1214
1049 337.1290 336.1217
1050 337.1291 336.1218
1051 337.1292 336.1219
1052 337.1293 336.1220
1053 337.1636 336.1563
1054 337.1641 336.1568
1055 337.1641 336.1568
1056 337.1642 336.1569
1057 337.1642 336.1569
1058 337.1643 336.1570
1059 337.1643 336.1570
1060 337.1643 336.1570
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