MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy1
MS data file : PRT1311_JBEI_1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 8 Sep 2025 at 17:17:19 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,450

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1101–1200 (out of 4217)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
861 331.1534 330.1461
862 331.1535 330.1462
863 331.1535 330.1462
864 331.1536 330.1463
865 331.1537 330.1464
866 331.1538 330.1465
867 331.1538 330.1465
868 331.1538 330.1465
869 331.1538 330.1465
870 331.1540 330.1467
871 331.1770 330.1697
872 331.1772 330.1699
873 331.1773 330.1700
874 331.1774 330.1701
875 331.1898 330.1825
876 331.1900 330.1827
877 331.1900 330.1827
878 331.1905 330.1832
879 331.1906 330.1833
880 331.1910 330.1837
881 332.1716 331.1643
882 332.1717 331.1644
883 332.1718 331.1645
884 332.2086 331.2013
885 333.0551 332.0478
886 333.0558 332.0485
887 333.0559 332.0486
888 333.0566 332.0493
889 333.0570 332.0497
890 333.0941 332.0868
891 333.0945 332.0872
892 333.0945 332.0872
893 333.1321 332.1248
894 333.1321 332.1248
895 333.1321 332.1248
896 333.1324 332.1251
897 333.1324 332.1251
898 333.1324 332.1251
899 333.1325 332.1252
900 333.1326 332.1253
901 333.1328 332.1255
902 333.1328 332.1255
903 333.1691 332.1618
904 333.1691 332.1618
905 333.1691 332.1618
906 333.1692 332.1619
907 333.1692 332.1619
908 333.1692 332.1619
909 333.1692 332.1619
910 333.1692 332.1619
911 333.1693 332.1620
912 333.1693 332.1620
913 333.1693 332.1620
914 333.1693 332.1620
915 333.1693 332.1620
916 333.1693 332.1620
917 333.1694 332.1621
918 333.1694 332.1621
919 333.1694 332.1621
920 333.1694 332.1621
921 333.1695 332.1622
922 333.1701 332.1628
923 333.1704 332.1631
924 333.2054 332.1981
925 333.2055 332.1982
926 333.2055 332.1982
927 333.2056 332.1983
928 333.2056 332.1983
929 333.2057 332.1984
930 333.2058 332.1985
931 333.2058 332.1985
932 333.2059 332.1986
933 333.2060 332.1987
934 333.2061 332.1988
935 333.2061 332.1988
936 333.2065 332.1992
937 333.2068 332.1995
938 333.2148 332.2075
939 333.2149 332.2076
940 333.2149 332.2076
941 333.2152 332.2079
942 333.2252 332.2179
943 333.2253 332.2180
944 333.2254 332.2181
945 333.2255 332.2182
946 333.2256 332.2183
947 333.2256 332.2183
948 333.2257 332.2184
949 333.2265 332.2192
950 333.2266 332.2193
951 334.0107 333.0034
952 334.0108 333.0035
953 334.0108 333.0035
954 335.0322 334.0249
955 335.0326 334.0253
956 335.0326 334.0253
957 335.1098 334.1025
958 335.1101 334.1028
959 335.1103 334.1030
960 335.1103 334.1030
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