MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy1
MS data file : PRT1311_JBEI_1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 8 Sep 2025 at 17:17:19 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,450

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 1001–1100 (out of 4217)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
761 326.1482 325.1409
762 326.1483 325.1410
763 326.1484 325.1411
764 326.1492 325.1419
765 327.0521 326.0448
766 327.1026 326.0953
767 327.1027 326.0954
768 327.1027 326.0954
769 327.1032 326.0959
770 327.1037 326.0964
771 327.1037 326.0964
772 327.1049 326.0976
773 327.1053 326.0980
774 327.1219 326.1146
775 327.1220 326.1147
776 327.1221 326.1148
777 327.1221 326.1148
778 327.1327 326.1254
779 327.1329 326.1256
780 327.1330 326.1257
781 327.1330 326.1257
782 327.1421 326.1348
783 327.1425 326.1352
784 327.1429 326.1356
785 327.1440 326.1367
786 327.1441 326.1368
787 327.1441 326.1368
788 327.1442 326.1369
789 327.1442 326.1369
790 327.1443 326.1370
791 327.1443 326.1370
792 327.1444 326.1371
793 327.1446 326.1373
794 327.1447 326.1374
795 327.1447 326.1374
796 327.1452 326.1379
797 327.1797 326.1724
798 327.1799 326.1726
799 327.1803 326.1730
800 327.1804 326.1731
801 327.1805 326.1732
802 327.1808 326.1735
803 327.1808 326.1735
804 327.1811 326.1738
805 327.1813 326.1740
806 327.1815 326.1742
807 327.2000 326.1927
808 327.2001 326.1928
809 327.2002 326.1929
810 327.2003 326.1930
811 327.2003 326.1930
812 327.2004 326.1931
813 327.2004 326.1931
814 327.2004 326.1931
815 327.2004 326.1931
816 327.2005 326.1932
817 327.2373 326.2300
818 327.9633 326.9560
819 328.0175 327.0102
820 328.0176 327.0103
821 328.0178 327.0105
822 329.0665 328.0592
823 329.0674 328.0601
824 329.0820 328.0747
825 329.0821 328.0748
826 329.0822 328.0749
827 329.0822 328.0749
828 329.0822 328.0749
829 329.0824 328.0751
830 329.1205 328.1132
831 329.1372 328.1299
832 329.1376 328.1303
833 329.1377 328.1304
834 329.1377 328.1304
835 329.1378 328.1305
836 329.1597 328.1524
837 329.1599 328.1526
838 329.1600 328.1527
839 329.1601 328.1528
840 329.1602 328.1529
841 329.1604 328.1531
842 329.1604 328.1531
843 329.1606 328.1533
844 329.1609 328.1536
845 329.1612 328.1539
846 329.9588 328.9515
847 330.0486 329.0413
848 331.0972 330.0899
849 331.0974 330.0901
850 331.0979 330.0906
851 331.1155 330.1082
852 331.1163 330.1090
853 331.1163 330.1090
854 331.1163 330.1090
855 331.1164 330.1091
856 331.1165 330.1092
857 331.1169 330.1096
858 331.1533 330.1460
859 331.1533 330.1460
860 331.1534 330.1461
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