MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy1
MS data file : PRT1311_JBEI_1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 8 Sep 2025 at 17:17:19 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,450

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 901–1000 (out of 4217)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
661 323.1483 322.1410
662 323.1483 322.1410
663 323.1483 322.1410
664 323.1483 322.1410
665 323.1484 322.1411
666 323.1484 322.1411
667 323.1484 322.1411
668 323.1484 322.1411
669 323.1484 322.1411
670 323.1484 322.1411
671 323.1484 322.1411
672 323.1484 322.1411
673 323.1486 322.1413
674 323.1486 322.1413
675 323.1487 322.1414
676 323.1488 322.1415
677 323.1668 322.1595
678 323.1854 322.1781
679 323.1855 322.1782
680 323.1855 322.1782
681 323.1855 322.1782
682 323.1856 322.1783
683 323.1856 322.1783
684 323.1856 322.1783
685 323.1856 322.1783
686 323.1856 322.1783
687 323.1856 322.1783
688 323.1856 322.1783
689 323.1857 322.1784
690 323.1857 322.1784
691 323.1857 322.1784
692 323.1857 322.1784
693 323.1858 322.1785
694 323.1859 322.1786
695 323.1859 322.1786
696 323.1859 322.1786
697 323.1859 322.1786
698 323.1860 322.1787
699 323.1863 322.1790
700 323.1869 322.1796
701 323.2046 322.1973
702 323.2047 322.1974
703 323.2047 322.1974
704 323.2048 322.1975
705 323.2049 322.1976
706 323.2050 322.1977
707 323.2057 322.1984
708 323.2791 322.2718
709 324.0168 323.0095
710 324.0168 323.0095
711 325.0865 324.0792
712 325.0874 324.0801
713 325.0875 324.0802
714 325.0890 324.0817
715 325.0894 324.0821
716 325.1268 324.1195
717 325.1268 324.1195
718 325.1269 324.1196
719 325.1272 324.1199
720 325.1272 324.1199
721 325.1275 324.1202
722 325.1275 324.1202
723 325.1277 324.1204
724 325.1277 324.1204
725 325.1277 324.1204
726 325.1277 324.1204
727 325.1280 324.1207
728 325.1282 324.1209
729 325.1286 324.1213
730 325.1643 324.1570
731 325.1644 324.1571
732 325.1644 324.1571
733 325.1647 324.1574
734 325.1647 324.1574
735 325.1648 324.1575
736 325.1649 324.1576
737 325.1824 324.1751
738 325.1825 324.1752
739 325.1826 324.1753
740 325.1827 324.1754
741 325.1828 324.1755
742 325.1828 324.1755
743 325.1830 324.1757
744 325.1830 324.1757
745 325.1832 324.1759
746 325.1838 324.1765
747 325.2016 324.1943
748 325.2019 324.1946
749 325.2021 324.1948
750 325.2022 324.1949
751 325.2023 324.1950
752 325.2212 324.2139
753 325.2213 324.2140
754 325.2216 324.2143
755 325.2223 324.2150
756 325.2951 324.2878
757 326.0123 325.0050
758 326.0124 325.0051
759 326.0125 325.0052
760 326.1480 325.1407
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