MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Andy1 |
| MS data file | : | PRT1311_JBEI_1.mgf |
| Database | : | Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues) |
| Timestamp | : | 8 Sep 2025 at 17:17:19 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,450 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| Score | Mass | Matches | Sequences | emPAI | |||
| 1 | Q88JG7_PSEPK | 2537 | 44735 | 113 (90) | 15 (14) | 3.27 | Fe-containing alcohol dehydrogenase OS=Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440) OX=160488 GN=yiaY PE=3 SV=1 |
| 2 | Q88JG6_PSEPK | 262 | 61597 | 23 (16) | 10 (7) | 0.29 | histidine kinase OS=Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440) OX=160488 GN=PP_2683 PE=4 SV=2 |
| 3 | TOLB_ECOLI | 239 | 45927 | 16 (11) | 7 (6) | 0.41 | Protein TolB OS=Escherichia coli (strain K12) GN=tolB PE=1 SV=1 |
| 4 | EFTU1_ECOLI | 221 | 43427 | 26 (13) | 11 (7) | 0.43 | Elongation factor Tu 1 OS=Escherichia coli (strain K12) GN=tufA PE=1 SV=1 |
| 5 | TRYP_PIG | 218 | 25078 | 9 (7) | 3 (2) | 0.19 | Trypsin OS=Sus scrofa PE=1 SV=1 |
| 6 | Q70I01_9ACTO | 133 | 166730 | 14 (6) | 4 (2) | 0.06 | Borrelidin polyketide synthase, type I OS=Streptomyces parvulus GN=borA2 PE=4 SV=1 |
| 7 | DNAJ_ECOLI | 102 | 41645 | 11 (7) | 7 (4) | 0.24 | Chaperone protein DnaJ OS=Escherichia coli (strain K12) GN=dnaJ PE=1 SV=3 |
| 8 | RECA_ECOLI | 61 | 38121 | 9 (3) | 6 (1) | 0.06 | Protein RecA OS=Escherichia coli (strain K12) GN=recA PE=1 SV=2 |
| 9 | K2C1_HUMAN | 52 | 66170 | 4 (1) | 3 (1) | 0.03 | Keratin, type II cytoskeletal 1 OS=Homo sapiens GN=KRT1 PE=1 SV=6 |
| 10 | YBBD_ECOLI | 49 | 0 | 3 (2) | 1 (1) | 0.26 | description |
| 11 | BTUF_ECOLI | 23 | 29463 | 2 (1) | 1 (1) | 0.08 | Vitamin B12-binding protein OS=Escherichia coli (strain K12) GN=btuF PE=1 SV=2 |
| 12 | MURC_ECOLI | 23 | 0 | 2 (1) | 1 (1) | 0.04 | description |
| 13 | YCBK_ECOLI | 21 | 20342 | 3 (1) | 1 (1) | 0.12 | Uncharacterized protein YcbK OS=Escherichia coli (strain K12) GN=ycbK PE=4 SV=1 |
| 14 | K1C10_HUMAN | 18 | 59020 | 7 (1) | 4 (1) | 0.04 | Keratin, type I cytoskeletal 10 OS=Homo sapiens GN=KRT10 PE=1 SV=6 |
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