MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Andy1 |
| MS data file | : | PRT1311_JBEI_1.mgf |
| Database | : | Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues) |
| Timestamp | : | 8 Sep 2025 at 17:17:19 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,450 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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1| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88JG7_PSEPK | 2537 | Fe-containing alcohol dehydrogenase OS=Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440) OX=160488 GN=yiaY PE=3 SV=1 |
2| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88JG6_PSEPK | 262 | histidine kinase OS=Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440) OX=160488 GN=PP_2683 PE=4 SV=2 |
3| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | TOLB_ECOLI | 239 | Protein TolB OS=Escherichia coli (strain K12) GN=tolB PE=1 SV=1 |
4| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | EFTU1_ECOLI | 221 | Elongation factor Tu 1 OS=Escherichia coli (strain K12) GN=tufA PE=1 SV=1 |
5| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | TRYP_PIG | 218 | Trypsin OS=Sus scrofa PE=1 SV=1 |
6| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q70I01_9ACTO | 133 | Borrelidin polyketide synthase, type I OS=Streptomyces parvulus GN=borA2 PE=4 SV=1 |
7| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | DNAJ_ECOLI | 102 | Chaperone protein DnaJ OS=Escherichia coli (strain K12) GN=dnaJ PE=1 SV=3 |
8| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RECA_ECOLI | 61 | Protein RecA OS=Escherichia coli (strain K12) GN=recA PE=1 SV=2 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 8.1 | RECA_ECOLI |
61 | 38121 | 9 (3) | 6 (1) | 0.06 | |
| Protein RecA OS=Escherichia coli (strain K12) GN=recA PE=1 SV=2 | |||||||
9 peptide matches (6 non-duplicate, 3 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
D:\Xcalibur\Data\Jennifer\PRT1311 Andy\PRT1311_JBEI_1.rawScore > 20 indicates identity Score > 18 indicates homology |
|||||||||||
| 6.53 | 1 | 5 | 1 | 1 | AEGFR | ||||||
| 6.53 | 0 | 5 | 1 | 1 | AQGFR + Deamidated (NQ) | ||||||
| 3044 |
579.2923 | 578.2850 | 578.2846 | 0.70 | 0 | 5 | 1 | 1Score > 20 indicates identity Score > 18 indicates homology |
U | K.GSIMR.L + Oxidation (M) | |
| 6.53 | 0 | 5 | 1 | 1 | QAGFR + Deamidated (NQ) | ||||||
| 0.70 | 0 | 5 | 1 | 1 | SVAMR + Oxidation (M) | ||||||
| 0.67 | 0 | 5 | 1 | 1 | TGVMR + Oxidation (M) | ||||||
| 0.70 | 0 | 5 | 1 | 1 | VASMR + Oxidation (M) | ||||||
| 0.67 | 0 | 5 | 1 | 1 | VTGMR + Oxidation (M) | ||||||
| 3057 |
308.1966 | 614.3786 | 614.3751 | 5.71 | 0 | 3 | 1 | 1Score > 20 indicates identity Score > 16 indicates homology |
U | K.LAGNLK.Q | |
| 3089 |
1 |
323.7015 | 645.3884 | 645.3850 | 5.38 | 0 | 8 | 0.64 | 1Score > 18 indicates identity |
U | K.IAAPFK.Q |
| 3128 |
371.6994 | 741.3842 | 741.3810 | 4.43 | 0 | 11 | 0.24 | 1Score > 17 indicates identity |
U | K.FYASVR.L | |
| 3384 |
2 |
542.8251 | 1083.6356 | 1083.6288 | 6.35 | 1 | 38 | 0.00037 | 1Score > 16 indicates identity |
U | K.ALAAALGQIEK.Q |
| 3787 |
870.9955 | 1739.9764 | 1739.9669 | 5.47 | 0 | 11 | 0.12 | 1Score > 15 indicates identity |
U | R.SGAVDVIVVDSVAALTPK.A | |
9| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | K2C1_HUMAN | 52 | Keratin, type II cytoskeletal 1 OS=Homo sapiens GN=KRT1 PE=1 SV=6 |
10| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | YBBD_ECOLI | 49 | description |
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