MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Andy1 |
| MS data file | : | PRT1311_JBEI_1.mgf |
| Database | : | Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues) |
| Timestamp | : | 8 Sep 2025 at 17:17:19 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,450 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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1| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88JG7_PSEPK | 2537 | Fe-containing alcohol dehydrogenase OS=Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440) OX=160488 GN=yiaY PE=3 SV=1 |
2| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88JG6_PSEPK | 262 | histidine kinase OS=Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440) OX=160488 GN=PP_2683 PE=4 SV=2 |
3| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | TOLB_ECOLI | 239 | Protein TolB OS=Escherichia coli (strain K12) GN=tolB PE=1 SV=1 |
4| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | EFTU1_ECOLI | 221 | Elongation factor Tu 1 OS=Escherichia coli (strain K12) GN=tufA PE=1 SV=1 |
5| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | TRYP_PIG | 218 | Trypsin OS=Sus scrofa PE=1 SV=1 |
6| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q70I01_9ACTO | 133 | Borrelidin polyketide synthase, type I OS=Streptomyces parvulus GN=borA2 PE=4 SV=1 |
7| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | DNAJ_ECOLI | 102 | Chaperone protein DnaJ OS=Escherichia coli (strain K12) GN=dnaJ PE=1 SV=3 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 7.1 | DNAJ_ECOLI |
102 | 41645 | 11 (7) | 7 (4) | 0.24 | |
| Chaperone protein DnaJ OS=Escherichia coli (strain K12) GN=dnaJ PE=1 SV=3 | |||||||
11 peptide matches (7 non-duplicate, 4 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 3208 |
2 |
457.2266 | 912.4386 | 912.4341 | 4.94 | 0 | 30 | 0.0046 | 1Score > 19 indicates identity |
U | K.FFDDLTR.- |
| 3226 |
309.5013 | 925.4821 | 925.4770 | 5.49 | 1 | 1 | 2.8 | 2Score > 18 indicates identity |
U | K.QHPIFER.E | |
| 3309 |
500.7597 | 999.5048 | 999.4985 | 6.32 | 0 | 36 | 0.0011 | 1Score > 19 indicates identity |
U | K.IPAGVDTGDR.I | |
D:\Xcalibur\Data\Jennifer\PRT1311 Andy\PRT1311_JBEI_1.rawScore > 22 indicates identity |
|||||||||||
| 3348 |
523.7590 | 1045.5034 | 1045.4975 | 5.71 | 0 | 50 | 8.9e-005 | 1Score > 22 indicates identity |
U | R.GGAQGDLLCR.V | |
| No other peptide matches in query | |||||||||||
| 3432 |
382.5400 | 1144.5982 | 1144.5910 | 6.24 | 1 | 15 | 0.2 | 1Score > 20 indicates identity |
U | R.GTLIKDPCNK.C | |
| 3524 |
2 |
423.9043 | 1268.6911 | 1268.6837 | 5.81 | 1 | 30 | 0.0043 | 1Score > 19 indicates identity |
U | K.IPAGVDTGDRIR.L |
| 3825 |
641.2961 | 1920.8665 | 1920.8360 | 15.9 | 0 | 12 | 0.27 | 1Score > 18 indicates identity |
U | R.QGFFAVQQTCPHCQGR.G + Deamidated (NQ) | |
8| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RECA_ECOLI | 61 | Protein RecA OS=Escherichia coli (strain K12) GN=recA PE=1 SV=2 |
9| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | K2C1_HUMAN | 52 | Keratin, type II cytoskeletal 1 OS=Homo sapiens GN=KRT1 PE=1 SV=6 |
10| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | YBBD_ECOLI | 49 | description |
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