MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Andy1 |
| MS data file | : | PRT1311_JBEI_1.mgf |
| Database | : | Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues) |
| Timestamp | : | 8 Sep 2025 at 17:17:19 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,450 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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1| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88JG7_PSEPK | 2537 | Fe-containing alcohol dehydrogenase OS=Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440) OX=160488 GN=yiaY PE=3 SV=1 |
2| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88JG6_PSEPK | 262 | histidine kinase OS=Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440) OX=160488 GN=PP_2683 PE=4 SV=2 |
3| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | TOLB_ECOLI | 239 | Protein TolB OS=Escherichia coli (strain K12) GN=tolB PE=1 SV=1 |
4| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | EFTU1_ECOLI | 221 | Elongation factor Tu 1 OS=Escherichia coli (strain K12) GN=tufA PE=1 SV=1 |
5| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | TRYP_PIG | 218 | Trypsin OS=Sus scrofa PE=1 SV=1 |
6| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q70I01_9ACTO | 133 | Borrelidin polyketide synthase, type I OS=Streptomyces parvulus GN=borA2 PE=4 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 6.1 | Q70I01_9ACTO |
133 | 166730 | 14 (6) | 4 (2) | 0.06 | |
| Borrelidin polyketide synthase, type I OS=Streptomyces parvulus GN=borA2 PE=4 SV=1 | |||||||
| 1 sameset of Q70I01_9ACTO | |||||||
| K4I6L4_ACP-AT-9ACTN |
133 | 263142 | 9 (6) | 3 (2) | 0.03 | ||
| Flv003-ACP-AT-FluC OS=Actinomadura vulgaris subsp. lanata OX=1233072 GN=fluC PE=4 SV=1 | |||||||
14 peptide matches (8 non-duplicate, 6 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 3731 |
1 |
521.9614 | 1562.8624 | 1562.8529 | 6.08 | 0 | 7 | 0.42 | 1Score > 16 indicates identity |
K.SNIGHAQAAAGVAGVIK.M | |
| 3832 |
977.4979 | 1952.9812 | 1952.9705 | 5.52 | 0 | 27 | 0.0091 | 1Score > 19 indicates identity |
U | R.GVASSASGGLAFVFAGQGSQR.L | |
| 3834 |
1 |
652.3359 | 1953.9859 | 1953.9545 | 16.1 | 0 | 34 | 0.002 | 1Score > 20 indicates identity |
U | R.GVASSASGGLAFVFAGQGSQR.L + Deamidated (NQ) |
| 3835 |
652.3367 | 1953.9883 | 1953.9545 | 17.3 | 0 | 6 | 1 | 1Score > 20 indicates identity Score > 18 indicates homology |
U | R.GVASSASGGLAFVFAGQGSQR.L + Deamidated (NQ) | |
| 3982 |
1080.5184 | 2159.0222 | 2158.9960 | 12.2 | 1 | 6 | 1.2 | 1Score > 19 indicates identity |
U | R.EGGFLDGAGQFDAAFFGISPR.E + Deamidated (NQ) | |
D:\Xcalibur\Data\Jennifer\PRT1311 Andy\PRT1311_JBEI_1.rawScore > 19 indicates identity |
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| 3983 |
2 |
720.6837 | 2159.0293 | 2158.9960 | 15.4 | 1 | 10 | 0.49 | 1Score > 19 indicates identity |
U | R.EGGFLDGAGQFDAAFFGISPR.E + Deamidated (NQ) |
| 2.12 | 1 | 10 | 0.49 | 1 | RLMQMMPQVLPSPDAMGPK + Deamidated (NQ); 2 Oxidation (M) | ||||||
| 2.12 | 1 | 3 | 2.3 | 3 | RLMQMMPQVLPSPDAMGPK + Deamidated (NQ); 2 Oxidation (M) | ||||||
| 2.12 | 1 | 3 | 2.4 | 4 | RLMQMMPQVLPSPDAMGPK + Deamidated (NQ); 2 Oxidation (M) | ||||||
| 4186 |
1 |
866.1358 | 2595.3856 | 2595.3486 | 14.3 | 0 | 53 | 9.8e-006 | 1Score > 15 indicates identity |
U | R.TVLSAAASLYVQGHPVDWAPLFPR.A + Deamidated (NQ) |
| 4187 |
1 |
649.8538 | 2595.3861 | 2595.3486 | 14.5 | 0 | 25 | 0.0062 | 1Score > 15 indicates identity |
U | R.TVLSAAASLYVQGHPVDWAPLFPR.A + Deamidated (NQ) |
7| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | DNAJ_ECOLI | 102 | Chaperone protein DnaJ OS=Escherichia coli (strain K12) GN=dnaJ PE=1 SV=3 |
8| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RECA_ECOLI | 61 | Protein RecA OS=Escherichia coli (strain K12) GN=recA PE=1 SV=2 |
9| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | K2C1_HUMAN | 52 | Keratin, type II cytoskeletal 1 OS=Homo sapiens GN=KRT1 PE=1 SV=6 |
10| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | YBBD_ECOLI | 49 | description |
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