MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Andy1 |
| MS data file | : | PRT1311_JBEI_1.mgf |
| Database | : | Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues) |
| Timestamp | : | 8 Sep 2025 at 17:17:19 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,450 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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1| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88JG7_PSEPK | 2537 | Fe-containing alcohol dehydrogenase OS=Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440) OX=160488 GN=yiaY PE=3 SV=1 |
2| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q88JG6_PSEPK | 262 | histidine kinase OS=Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440) OX=160488 GN=PP_2683 PE=4 SV=2 |
3| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | TOLB_ECOLI | 239 | Protein TolB OS=Escherichia coli (strain K12) GN=tolB PE=1 SV=1 |
4| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | EFTU1_ECOLI | 221 | Elongation factor Tu 1 OS=Escherichia coli (strain K12) GN=tufA PE=1 SV=1 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 4.1 | EFTU1_ECOLI |
221 | 43427 | 26 (13) | 11 (7) | 0.43 | |
| Elongation factor Tu 1 OS=Escherichia coli (strain K12) GN=tufA PE=1 SV=1 | |||||||
| 1 sameset of EFTU1_ECOLI | |||||||
| EFTU2_ECOLI |
221 | 43457 | 26 (13) | 11 (7) | 0.43 | ||
| Elongation factor Tu 2 OS=Escherichia coli (strain K12) GN=tufB PE=1 SV=1 | |||||||
26 peptide matches (12 non-duplicate, 14 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
| 3119 |
1 |
351.6944 | 701.3742 | 701.3708 | 4.93 | 1 | 22 | 0.064 | 1Score > 23 indicates identity |
U | K.LLDEGR.A |
| 3138 |
2 |
388.7103 | 775.4060 | 775.4017 | 5.58 | 0 | 23 | 0.014 | 1Score > 20 indicates identity Score > 17 indicates homology |
U | R.HTPFFK.G |
| 3154 |
401.2477 | 800.4808 | 800.4756 | 6.54 | 0 | 16 | 0.12 | 1Score > 19 indicates identity |
U | R.TVGAGVVAK.V | |
| 3177 |
1 |
419.2532 | 836.4918 | 836.4868 | 6.02 | 1 | 21 | 0.011 | 1Score > 14 indicates identity |
U | R.EHILLGR.Q |
| 3333 |
2 |
514.3016 | 1026.5886 | 1026.5822 | 6.30 | 1 | 76 | 9.2e-008 | 1Score > 18 indicates identity |
U | R.AGENVGVLLR.G |
| 3489 |
617.3147 | 1232.6148 | 1232.6091 | 4.69 | 0 | 2 | 1 | 1Score > 22 indicates identity Score > 15 indicates homology |
U | K.GYRPQFYFR.T | |
| 3492 |
1 |
411.8792 | 1232.6158 | 1232.6091 | 5.44 | 0 | 14 | 0.059 | 1Score > 22 indicates identity Score > 14 indicates homology |
U | K.GYRPQFYFR.T |
| 3547 |
2 |
652.3977 | 1302.7808 | 1302.7759 | 3.83 | 0 | 23 | 0.0046 | 1Score > 13 indicates identity |
U | K.TTLTAAITTVLAK.T |
| 3801 |
1 |
601.9707 | 1802.8903 | 1802.8799 | 5.75 | 1 | 53 | 3e-005 | 1Score > 20 indicates identity |
U | R.GITINTSHVEYDTPTR.H |
| 3806 |
1 |
604.9869 | 1811.9389 | 1811.9274 | 6.34 | 0 | 21 | 0.036 | 1Score > 19 indicates identity |
U | K.MVVTLIHPIAMDDGLR.F + 2 Oxidation (M) |
| 3848 |
2 |
983.4890 | 1964.9634 | 1964.9367 | 13.6 | 1 | 46 | 0.00014 | 1Score > 20 indicates identity |
U | R.ELLSQYDFPGDDTPIVR.G + Deamidated (NQ) |
D:\Xcalibur\Data\Jennifer\PRT1311 Andy\PRT1311_JBEI_1.rawScore > 19 indicates identity |
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| 4206 |
1 |
910.7897 | 2729.3473 | 2729.3186 | 10.5 | 0 | 19 | 0.059 | 1Score > 19 indicates identity |
U | K.NMITGAAQMDGAILVVAATDGPMPQTR.E + Deamidated (NQ) |
| 10.5 | 0 | 19 | 0.059 | 1 | K.NMITGAAQMDGAILVVAATDGPMPQTR.E + Deamidated (NQ) | ||||||
5| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | TRYP_PIG | 218 | Trypsin OS=Sus scrofa PE=1 SV=1 |
6| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | Q70I01_9ACTO | 133 | Borrelidin polyketide synthase, type I OS=Streptomyces parvulus GN=borA2 PE=4 SV=1 |
7| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | DNAJ_ECOLI | 102 | Chaperone protein DnaJ OS=Escherichia coli (strain K12) GN=dnaJ PE=1 SV=3 |
8| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | RECA_ECOLI | 61 | Protein RecA OS=Escherichia coli (strain K12) GN=recA PE=1 SV=2 |
9| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | K2C1_HUMAN | 52 | Keratin, type II cytoskeletal 1 OS=Homo sapiens GN=KRT1 PE=1 SV=6 |
10| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | YBBD_ECOLI | 49 | description |
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