MASCOT Search ResultsMASCOT Search Results| User | : | Jennifer |
|---|---|---|
| : | [email protected] | |
| Search title | : | Andy1 |
| MS data file | : | PRT1311_JBEI_1.mgf |
| Database | : | Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues) |
| Timestamp | : | 8 Sep 2025 at 17:17:19 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | GluC_Trypsin |
| Fixed modifications | : | Carbamidomethyl (C) |
| Variable modifications | : | Deamidated (NQ), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm |
| Fragment mass tolerance | : | ± 0.1 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-FTICR |
| Number of queries | : | 4,450 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).
[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-005 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-006 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-007 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-005 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-005 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
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11| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | BTUF_ECOLI | 23 | Vitamin B12-binding protein OS=Escherichia coli (strain K12) GN=btuF PE=1 SV=2 |
12| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | MURC_ECOLI | 23 | description |
13| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | YCBK_ECOLI | 21 | Uncharacterized protein YcbK OS=Escherichia coli (strain K12) GN=ycbK PE=4 SV=1 |
14| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | K1C10_HUMAN | 18 | Keratin, type I cytoskeletal 10 OS=Homo sapiens GN=KRT10 PE=1 SV=6 |
| Score | Mass | Matches | Sequences | emPAI | |||
|---|---|---|---|---|---|---|---|
| 14.1 | K1C10_HUMAN |
18 | 59020 | 7 (1) | 4 (1) | 0.04 | |
| Keratin, type I cytoskeletal 10 OS=Homo sapiens GN=KRT10 PE=1 SV=6 | |||||||
7 peptide matches (4 non-duplicate, 3 duplicate)| Query | Dupes | Observed | Mr(expt) | Mr(calc) | ppm | M | Score | Expect | Rank | U | Peptide |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 3145 |
1 |
785.4618 | 784.4545 | 784.4555 | -1.27 | 0 | 14 | 0.17 | 7Score > 19 indicates identity |
U | E.QLQQIR.A |
| 3159 |
1 |
404.2067 | 806.3988 | 806.3923 | 8.17 | 0 | 8 | 1 | 1Score > 21 indicates identity Score > 21 indicates homology |
R.LAADDFR.L | |
| 3585 |
1 |
712.8619 | 1423.7092 | 1423.6830 | 18.4 | 1 | 1 | 1.1 | 3Score > 20 indicates identity Score > 14 indicates homology |
U | E.IDNNIEQISSYK.S + Deamidated (NQ) |
| 4259 |
1018.5601 | 3052.6585 | 3052.6040 | 17.8 | 1 | 18 | 0.019 | 1Score > 13 indicates identity |
U | K.TIDDLKNQILNLTTDNANILLQIDNAR.L + Deamidated (NQ) |
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