MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Andy1
MS data file : PRT1311_JBEI_1.mgf
Database : Ecoli-MetEng 20200720 (4,900 sequences; 1,661,431 residues)
Timestamp : 8 Sep 2025 at 17:17:19 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,450

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 19 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Protein families 1–10 (out of 14)

Page: 1 2 Next 

+1

Accession Score Description
1 Q88JG7_PSEPK 2537 Fe-containing alcohol dehydrogenase OS=Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440) OX=160488 GN=yiaY PE=3 SV=1

+2

Accession Score Description
1 Q88JG6_PSEPK 262 histidine kinase OS=Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440) OX=160488 GN=PP_2683 PE=4 SV=2

+3

Accession Score Description
1 TOLB_ECOLI 239 Protein TolB OS=Escherichia coli (strain K12) GN=tolB PE=1 SV=1

+4

Accession Score Description
1 EFTU1_ECOLI 221 Elongation factor Tu 1 OS=Escherichia coli (strain K12) GN=tufA PE=1 SV=1

+5

Accession Score Description
1 TRYP_PIG 218 Trypsin OS=Sus scrofa PE=1 SV=1

+6

Accession Score Description
1 Q70I01_9ACTO 133 Borrelidin polyketide synthase, type I OS=Streptomyces parvulus GN=borA2 PE=4 SV=1

+7

Accession Score Description
1 DNAJ_ECOLI 102 Chaperone protein DnaJ OS=Escherichia coli (strain K12) GN=dnaJ PE=1 SV=3

+8

Accession Score Description
1 RECA_ECOLI 61 Protein RecA OS=Escherichia coli (strain K12) GN=recA PE=1 SV=2

+9

Accession Score Description
1 K2C1_HUMAN 52 Keratin, type II cytoskeletal 1 OS=Homo sapiens GN=KRT1 PE=1 SV=6

-10

Accession Score Description
1 YBBD_ECOLI 49 description
Score Mass Matches Sequences emPAI
10.1 YBBD_ECOLI 49 0 3 (2) 1 (1) 0.26
description

-3 peptide matches (1 non-duplicate, 2 duplicate)

Query Dupes Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank U Peptide
3009 -2 533.3330 532.3257 532.3221 6.90 0 39 0.00024 +1Score > 15 indicates identity U E.SLVSK.H
3007   533.3322 532.3249 532.3221 5.40 0 (14) 0.068 +1Score > 15 indicates identity U E.SLVSK.H
3008   533.3329 532.3256 532.3221 6.71 0 (26) 0.0046 +1Score > 15 indicates identity U E.SLVSK.H

1 subset or intersection (2 subset proteins in total)

Score Mass Subset of
Q6M2X6_CORGL 36 0 10.1
description
1 sameset of Q6M2X6_CORGL
Q8NMS0_CORGL 36 0
description
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