MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita6 sp
MS data file : PRT1270_T-BRSC_6_20250714122635.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:38:56 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,869

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 801–900 (out of 3786)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
2973 923.4497 1844.8848 1844.8839 0.50 1 15 0.1 +1Score > 40 indicates identity
Score > 18 indicates homology		 SHVTLPQIRGMFNGDR + 2 Deamidated (NQ); Oxidation (M)
771 421.7234 841.4322 841.4406 -9.92 0 15 0.79 +1Score > 39 indicates identity
Score > 27 indicates homology		 NTPNQIR
1147 332.8549 995.5429 995.5440 -1.14 1 15 0.43 +1Score > 37 indicates identity
Score > 24 indicates homology		 WTYKTGLK
1576 594.8222 1187.6298 1187.6397 -8.32 1 15 0.7 +1Score > 41 indicates identity
Score > 26 indicates homology		 LAEALTISQNK + Deamidated (NQ)
2232 738.9006 1475.7866 1475.7653 14.5 1 15 0.25 +1Score > 40 indicates identity
Score > 22 indicates homology		 DNIKIMSALINAR + 2 Deamidated (NQ); Oxidation (M)
2749 571.6672 1711.9798 1711.9608 11.1 1 15 0.36 +1Score > 34 indicates identity
Score > 23 indicates homology		 QQPITTTLELVDIIK + Deamidated (NQ)
1519 389.5573 1165.6501 1165.6567 -5.72 1 15 0.49 +1Score > 37 indicates identity
Score > 25 indicates homology		 KLGDHLTNLR
1831 644.8527 1287.6908 1287.6783 9.77 0 15 0.71 +1Score > 41 indicates identity
Score > 26 indicates homology		 VTQANVSNVLSR + Deamidated (NQ)
2065 699.8886 1397.7626 1397.7514 8.04 1 15 0.85 +1Score > 39 indicates identity
Score > 27 indicates homology		 VLALLNDNLQRE + Deamidated (NQ)
397 333.1890 664.3634 664.3504 19.7 1 15 0.33 +1Score > 39 indicates identity
Score > 23 indicates homology		 SSTRSK
3222 671.3552 2011.0438 2011.0262 8.74 1 15 0.42 +1Score > 39 indicates identity
Score > 24 indicates homology		 QTLSPSVLIEHLNLFGNE + Deamidated (NQ)
2784 576.9919 1727.9539 1727.9206 19.3 1 15 0.23 +1Score > 37 indicates identity
Score > 21 indicates homology		 FNTLHQLLRTLQDK + 2 Deamidated (NQ)
1542 586.8538 1171.6930 1171.6812 10.1 1 15 0.52 +1Score > 36 indicates identity
Score > 25 indicates homology		 LTLKLQTLNE
3366 705.3883 2113.1431 2113.1380 2.39 0 15 0.16 +1Score > 36 indicates identity
Score > 20 indicates homology		 WNQLDLAIVLLSIMGIALE + 2 Deamidated (NQ)
718 407.2282 812.4418 812.4504 -10.6 1 15 0.16 +1Score > 39 indicates identity
Score > 20 indicates homology		 KAPASSPR
1265 349.8411 1046.5015 1046.5179 -15.7 1 15 0.77 +1Score > 42 indicates identity
Score > 26 indicates homology		 KGTPGDLMGR + Oxidation (M)
1516 389.2171 1164.6295 1164.6073 19.0 1 15 0.96 +1Score > 39 indicates identity
Score > 27 indicates homology		 YINPSRIMR + Oxidation (M)
2156 718.9119 1435.8092 1435.7922 11.9 1 15 0.53 +1Score > 36 indicates identity
Score > 25 indicates homology		 IATQESLITGIYK
2034 693.8627 1385.7108 1385.7375 -19.2 1 15 0.36 +1Score > 41 indicates identity
Score > 23 indicates homology		 NAAAGTLRQLDTR
815 429.2231 856.4316 856.4403 -10.1 1 15 0.76 +1Score > 39 indicates identity
Score > 26 indicates homology		 LQPAEGSR
1875 654.8463 1307.6780 1307.6721 4.55 1 15 0.27 +1Score > 40 indicates identity
Score > 22 indicates homology		 LYELIDSNSVR
2007 456.5702 1366.6888 1366.6915 -1.98 0 15 0.46 +1Score > 40 indicates identity
Score > 24 indicates homology		 GTYVCIATNALGK
2643 832.4598 1662.9050 1662.8981 4.19 1 15 0.29 +1Score > 37 indicates identity
Score > 22 indicates homology		 IGNQIDLLFLKFNE
607 380.7238 759.4330 759.4279 6.78 0 15 0.92 +1Score > 40 indicates identity
Score > 27 indicates homology		 WNSIIK
3189 996.0222 1990.0298 1990.0081 10.9 1 15 0.16 +1Score > 39 indicates identity
Score > 20 indicates homology		 QTTEPNYPEMLLIAINK + Oxidation (M)
4290 976.5315 2926.5727 2926.6254 -18.0 1 15 1 +1Score > 33 indicates identity
Score > 28 indicates homology		 ARIAGVNIPNHQHTVIGLTAIFGIGTTR
1005 314.8367 941.4883 941.4930 -5.05 0 15 0.67 +1Score > 40 indicates identity
Score > 26 indicates homology		 VLSNAPDAR
1929 665.3479 1328.6812 1328.6936 -9.29 1 15 0.44 +1Score > 41 indicates identity
Score > 24 indicates homology		 QVVVNLLGSRGGE + 2 Deamidated (NQ)
889 445.7256 889.4366 889.4393 -2.93 1 15 0.5 +1Score > 42 indicates identity
Score > 24 indicates homology		 ELSAQDVK + Deamidated (NQ)
3362 704.3729 2110.0969 2110.0921 2.26 1 15 0.15 +1Score > 38 indicates identity
Score > 19 indicates homology		 LLFNDIIFRMPNPNYVK + Deamidated (NQ); Oxidation (M)
3712 769.0380 2304.0922 2304.0586 14.6 1 15 0.41 +1Score > 39 indicates identity
Score > 24 indicates homology		 NKIFQNFFPDLFSSLQNDE + 2 Deamidated (NQ)
2295 502.5964 1504.7674 1504.7643 2.07 1 15 0.68 +1Score > 41 indicates identity
Score > 26 indicates homology		 WALQAMGKMAGGIR + Oxidation (M)
3426 712.7402 2135.1988 2135.1699 13.5 1 15 0.22 +1Score > 32 indicates identity
Score > 21 indicates homology		 LSVAAVNGPRSVVVSGEPGALR + Deamidated (NQ)
2809 871.9969 1741.9792 1741.9475 18.2 0 15 0.59 +1Score > 35 indicates identity
Score > 25 indicates homology		 QLVQQGLGIFGGAIADR
2572 407.4531 1625.7833 1625.7937 -6.39 1 15 0.55 +1Score > 40 indicates identity
Score > 25 indicates homology		 IFDEYNLDAVTIGR + Deamidated (NQ)
3420 1068.5615 2135.1084 2135.1409 -15.2 1 15 0.28 +1Score > 38 indicates identity
Score > 22 indicates homology		 LADGAIDLYAMVVVLSRASR + Oxidation (M)
2483 794.4098 1586.8050 1586.8093 -2.68 1 15 0.85 +1Score > 40 indicates identity
Score > 27 indicates homology		 LKGFWGQDVAPIQE
1129 495.2801 988.5456 988.5375 8.22 0 15 0.75 +1Score > 40 indicates identity
Score > 26 indicates homology		 MNILIVNR + Deamidated (NQ); Oxidation (M)
2059 695.8885 1389.7624 1389.7365 18.7 0 15 0.69 +1Score > 39 indicates identity
Score > 26 indicates homology		 QPLGGPLGGINPNR + Deamidated (NQ)
3059 947.4986 1892.9826 1892.9731 5.05 1 15 0.81 +1Score > 39 indicates identity
Score > 27 indicates homology		 NFNVSVNILQGKISQTK + 4 Deamidated (NQ)
618 382.7134 763.4122 763.4228 -13.8 0 15 0.29 +1Score > 41 indicates identity
Score > 22 indicates homology		 NAVAVYK
1708 618.3073 1234.6000 1234.5975 2.02 1 15 0.41 +1Score > 42 indicates identity
Score > 24 indicates homology		 KSAMQAGSDLAR + Deamidated (NQ)
2371 772.4069 1542.7992 1542.8140 -9.59 0 15 0.55 +1Score > 40 indicates identity
Score > 25 indicates homology		 LAIQNSQIASAIALE + 2 Deamidated (NQ)
2397 519.3005 1554.8797 1554.8617 11.6 0 15 0.57 +1Score > 36 indicates identity
Score > 25 indicates homology		 VTQLLSLLANVQQE
2932 604.3597 1810.0573 1810.0352 12.2 0 15 0.76 +1Score > 30 indicates identity
Score > 26 indicates homology		 QNQTIILALALQWAVK + Deamidated (NQ)
3406 1064.5637 2127.1128 2127.0749 17.8 1 15 0.6 +1Score > 38 indicates identity
Score > 25 indicates homology		 IHSTFTIQVEYGTGPHAIR + Deamidated (NQ)
3718 769.7098 2306.1076 2306.1464 -16.8 1 15 0.18 +1Score > 39 indicates identity
Score > 20 indicates homology		 FQSDLKIINDCLDGLIQNAK + 2 Deamidated (NQ)
1146 332.8548 995.5426 995.5400 2.59 1 15 0.62 +1Score > 37 indicates identity
Score > 25 indicates homology		 EGQVTPLPR
1547 587.3134 1172.6122 1172.6037 7.30 1 15 0.95 +1Score > 41 indicates identity
Score > 27 indicates homology		 QLDNGIVKQR + 3 Deamidated (NQ)
816 429.2236 856.4326 856.4290 4.20 0 15 0.56 +1Score > 39 indicates identity
Score > 25 indicates homology		 AGVGVSAAPE
3450 1076.5603 2151.1060 2151.0848 9.90 1 15 0.18 +1Score > 38 indicates identity
Score > 20 indicates homology		 KPHTTTVAPPQINFIKNNE + 3 Deamidated (NQ)
568 368.7280 735.4414 735.4391 3.13 0 15 0.89 +1Score > 34 indicates identity
Score > 27 indicates homology		 LPHTIR
2050 695.8283 1389.6420 1389.6623 -14.6 1 15 0.66 +1Score > 40 indicates identity
Score > 25 indicates homology		 ESGVDANLSSLLGE
780 422.7452 843.4758 843.4888 -15.3 1 15 0.85 +1Score > 43 indicates identity
Score > 27 indicates homology		 INLKMPK + Deamidated (NQ)
1358 366.8922 1097.6548 1097.6444 9.41 0 15 0.79 +1Score > 37 indicates identity
Score > 26 indicates homology		 VVQNVINALK + Deamidated (NQ)
1641 405.8958 1214.6656 1214.6507 12.3 0 15 0.25 +1Score > 40 indicates identity
Score > 21 indicates homology		 ALIAGNIGTVASE
3672 759.3495 2275.0267 2275.0249 0.77 0 15 0.12 +1Score > 37 indicates identity
Score > 18 indicates homology		 WQGCDQMALVAQLSPGQLQE + Deamidated (NQ); Oxidation (M)
1987 454.2553 1359.7441 1359.7259 13.4 0 15 0.25 +1Score > 39 indicates identity
Score > 21 indicates homology		 VLDNHTALAHIR + Deamidated (NQ)
3674 570.0474 2276.1605 2276.1424 7.97 0 15 0.099 +1Score > 39 indicates identity
Score > 17 indicates homology		 TTLNLTLIDSPGLLFDQSQAE + Deamidated (NQ)
2025 690.3358 1378.6570 1378.6472 7.12 1 15 0.97 +1Score > 41 indicates identity
Score > 27 indicates homology		 TCLQMPGISEVK + Deamidated (NQ); Oxidation (M)
1916 442.2671 1323.7795 1323.7809 -1.07 1 15 0.42 +1Score > 34 indicates identity
Score > 23 indicates homology		 GVIAMLARQLPR
2625 825.9308 1649.8470 1649.8334 8.25 1 15 0.58 +1Score > 40 indicates identity
Score > 25 indicates homology		 AVLLMAAFELTQQAE + Oxidation (M)
1451 569.3577 1136.7008 1136.6805 17.9 0 15 0.28 +1Score > 30 indicates identity
Score > 22 indicates homology		 QIIAPALVALE
1946 671.3813 1340.7480 1340.7551 -5.27 0 15 0.55 +1Score > 38 indicates identity
Score > 25 indicates homology		 LNPSLVISLSQGL + Deamidated (NQ)
399 333.6776 665.3406 665.3384 3.32 0 15 0.93 +1Score > 41 indicates identity
Score > 27 indicates homology		 DVASFK
416 336.2197 670.4248 670.4126 18.3 1 15 0.7 +1Score > 40 indicates identity
Score > 26 indicates homology		 RVTAPK
1655 610.3287 1218.6428 1218.6390 3.15 1 15 0.23 +1Score > 41 indicates identity
Score > 21 indicates homology		 AALASMQRLNK + Deamidated (NQ); Oxidation (M)
1941 669.8262 1337.6378 1337.6503 -9.33 1 15 0.77 +1Score > 41 indicates identity
Score > 26 indicates homology		 NYVVLQPTEFE
564 367.7147 733.4148 733.4123 3.53 0 15 0.99 +1Score > 40 indicates identity
Score > 27 indicates homology		 FADILR
3932 832.4506 2494.3300 2494.3067 9.32 1 15 0.14 +1Score > 35 indicates identity
Score > 19 indicates homology		 TSAYIDVPTLVRNTILDIGYNR + Deamidated (NQ)
2530 806.9333 1611.8520 1611.8216 18.9 1 15 0.49 +1Score > 39 indicates identity
Score > 24 indicates homology		 QEDQVNVSLQSLPR
1468 381.8937 1142.6593 1142.6547 4.03 1 15 0.64 +1Score > 39 indicates identity
Score > 25 indicates homology		 KLALDLVDQK + Deamidated (NQ)
950 460.2531 918.4916 918.4771 15.9 0 15 0.97 +1Score > 41 indicates identity
Score > 27 indicates homology		 LTDIAGSSR
1105 487.3131 972.6116 972.5968 15.3 1 14 0.28 +1Score > 30 indicates identity
Score > 21 indicates homology		 ILKTGISNK
893 446.7440 891.4734 891.4637 11.0 0 14 0.88 +1Score > 41 indicates identity
Score > 26 indicates homology		 VVGMFPSR
1854 649.3488 1296.6830 1296.6898 -5.22 1 14 0.13 +1Score > 40 indicates identity
Score > 18 indicates homology		 NVTPNNNIRQK
3498 727.0822 2178.2248 2178.2082 7.61 0 14 0.095 +1Score > 32 indicates identity
Score > 17 indicates homology		 CNLSTVPGLALAQLPALVALR + 2 Deamidated (NQ)
1338 362.5463 1084.6171 1084.6142 2.69 1 14 0.81 +1Score > 39 indicates identity
Score > 26 indicates homology		 QGAKGLAWVR
1957 673.3621 1344.7096 1344.6959 10.2 1 14 0.31 +1Score > 41 indicates identity
Score > 22 indicates homology		 AIDLLGSKTAPME
1233 345.5026 1033.4860 1033.5040 -17.4 1 14 0.98 +1Score > 41 indicates identity
Score > 27 indicates homology		 DLESSASAVR
592 377.7517 753.4888 753.4861 3.67 1 14 0.38 +1Score > 32 indicates identity
Score > 23 indicates homology		 LGKLPAR
2067 700.3289 1398.6432 1398.6514 -5.84 0 14 0.94 +1Score > 40 indicates identity
Score > 27 indicates homology		 IIHQITDLNNSE + 3 Deamidated (NQ)
2496 532.2679 1593.7819 1593.7787 1.98 0 14 0.98 +1Score > 41 indicates identity
Score > 27 indicates homology		 SFFDAPANQTVLQR + Deamidated (NQ)
433 338.1837 674.3528 674.3460 10.2 1 14 0.83 +1Score > 43 indicates identity
Score > 26 indicates homology		 TDGRAR
2085 468.9143 1403.7211 1403.7369 -11.2 1 14 0.24 +1Score > 41 indicates identity
Score > 21 indicates homology		 TPAKGGVTVSSSASR
1630 606.3564 1210.6982 1210.6809 14.3 1 14 0.93 +1Score > 36 indicates identity
Score > 27 indicates homology		 VIVPEAITIQE
1778 422.9199 1265.7379 1265.7231 11.7 1 14 0.56 +1Score > 35 indicates identity
Score > 24 indicates homology		 LPISLPNGVEVK + Deamidated (NQ)
2335 759.9387 1517.8628 1517.8705 -5.02 1 14 0.52 +1Score > 35 indicates identity
Score > 24 indicates homology		 ISLVNIKYLVLDE
294 602.3586 601.3513 601.3435 13.0 1 14 0.95 +1Score > 45 indicates identity
Score > 27 indicates homology		 EAAAIK
982 310.1643 927.4711 927.4774 -6.79 0 14 0.55 +1Score > 40 indicates identity
Score > 24 indicates homology		 QQSQAPLR + Deamidated (NQ)
1478 384.1885 1149.5437 1149.5423 1.18 1 14 0.82 +1Score > 42 indicates identity
Score > 26 indicates homology		 MFPGGKMNPR + Oxidation (M)
2609 820.9221 1639.8296 1639.8279 1.04 0 14 0.28 +1Score > 40 indicates identity
Score > 21 indicates homology		 WIAYAQSLTSKPMK + Deamidated (NQ); Oxidation (M)
2790 866.8896 1731.7646 1731.7807 -9.30 0 14 0.33 +1Score > 39 indicates identity
Score > 22 indicates homology		 NDNPLVDMQAMSPTAK + Deamidated (NQ)
1245 518.2982 1034.5818 1034.5648 16.5 1 14 0.72 +1Score > 38 indicates identity
Score > 25 indicates homology		 AIIKYQGLE + Deamidated (NQ)
583 375.1999 748.3852 748.3827 3.36 1 14 0.82 +1Score > 42 indicates identity
Score > 26 indicates homology		 SSRSNAK
400 334.1739 666.3332 666.3337 -0.65 1 14 0.95 +1Score > 42 indicates identity
Score > 27 indicates homology		 YETVR
1777 633.8368 1265.6590 1265.6503 6.90 0 14 0.48 +1Score > 40 indicates identity
Score > 24 indicates homology		 VTYDIQASLQK + Deamidated (NQ)
2316 379.2159 1512.8345 1512.8260 5.61 0 14 0.73 +1Score > 38 indicates identity
Score > 25 indicates homology		 LASNVNLSLGVVDGR
207 501.3163 500.3090 500.3071 3.88 0 14 0.34 +1Score > 43 indicates identity
Score > 22 indicates homology		 AVVGR
1591 597.8575 1193.7004 1193.7132 -10.7 0 14 0.69 +1Score > 34 indicates identity
Score > 25 indicates homology		 NVPLAPLTTLR
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