MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita6 sp
MS data file : PRT1270_T-BRSC_6_20250714122635.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:38:56 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,869

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 601–700 (out of 3786)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
691 398.7160 795.4174 795.4127 6.02 0 18 0.54 +1Score > 38 indicates identity
Score > 27 indicates homology
ASLGSFSK
2246 743.3759 1484.7372 1484.7260 7.61 0 17 0.6 +1Score > 41 indicates identity
Score > 28 indicates homology
LWDDGIIDPANTR
1621 603.2940 1204.5734 1204.5935 -16.7 1 17 0.88 +1Score > 41 indicates identity
Score > 29 indicates homology
EGLSTVNSIQR + 2 Deamidated (NQ)
1229 1031.5135 1030.5062 1030.5196 -13.0 0 17 0.54 +1Score > 41 indicates identity
Score > 27 indicates homology
NGVWSIAAGR + Deamidated (NQ)
1282 353.5287 1057.5643 1057.5768 -11.8 1 17 0.54 +1Score > 42 indicates identity
Score > 27 indicates homology
NTVDVEILR
1053 479.2555 956.4964 956.4927 3.91 1 17 0.71 +1Score > 40 indicates identity
Score > 28 indicates homology
TPVAEDVAR
346 315.1852 628.3558 628.3544 2.26 0 17 0.78 +1Score > 37 indicates identity
Score > 29 indicates homology
LGGGTPK
506 354.1779 706.3412 706.3286 17.9 0 17 0.65 +1Score > 41 indicates identity
Score > 28 indicates homology
QAVFNE
1017 473.2806 944.5466 944.5443 2.44 0 17 0.24 +1Score > 40 indicates identity
Score > 24 indicates homology
IYPGQVLR
1115 491.7418 981.4690 981.4556 13.7 1 17 0.52 +1Score > 41 indicates identity
Score > 27 indicates homology
WEQYVTR + Deamidated (NQ)
1484 385.5655 1153.6747 1153.6567 15.5 1 17 0.57 +1Score > 36 indicates identity
Score > 27 indicates homology
ITGINDPRLR
687 398.2000 794.3854 794.3810 5.58 1 17 0.68 +1Score > 41 indicates identity
Score > 28 indicates homology
AVFENSK + Deamidated (NQ)
3265 680.6824 2039.0254 2038.9901 17.3 1 17 0.11 +1Score > 39 indicates identity
Score > 20 indicates homology
GFGFITPESGNDLFVHFR
1947 671.3813 1340.7480 1340.7551 -5.27 0 17 0.29 +1Score > 38 indicates identity
Score > 24 indicates homology
LNPSLVISLSQGL + Deamidated (NQ)
3679 761.3997 2281.1773 2281.1372 17.6 1 17 0.088 +1Score > 38 indicates identity
Score > 19 indicates homology
MTGALNNSGTTAIFINQLRDK + Deamidated (NQ); Oxidation (M)
3287 686.3705 2056.0897 2056.0550 16.8 0 17 0.17 +1Score > 37 indicates identity
Score > 22 indicates homology
YLQDQLIIFMALASGISR + 2 Deamidated (NQ); Oxidation (M)
1381 554.8015 1107.5884 1107.5812 6.58 1 17 0.59 +1Score > 40 indicates identity
Score > 27 indicates homology
AASFEQTILK + Deamidated (NQ)
2523 805.3809 1608.7472 1608.7600 -7.91 0 17 0.89 +1Score > 40 indicates identity
Score > 29 indicates homology
MGNTVAMILAGGQGTR + Deamidated (NQ); 2 Oxidation (M)
3054 630.6956 1889.0650 1889.0590 3.14 1 17 0.48 +1Score > 33 indicates identity
Score > 27 indicates homology
TLKPVLMAMINRTITR + 2 Oxidation (M)
1995 455.2505 1362.7297 1362.7116 13.2 1 17 0.22 +1Score > 40 indicates identity
Score > 23 indicates homology
EPQTKPSRHQR
1131 495.2818 988.5490 988.5488 0.28 1 17 0.83 +1Score > 40 indicates identity
Score > 29 indicates homology
MLLGDLRR + Oxidation (M)
2528 537.3425 1609.0057 1608.9814 15.1 1 17 0.14 +1Score > 21 indicates identity LSPAEVALITTLLLR
853 437.2633 872.5120 872.4967 17.6 1 17 0.46 +1Score > 40 indicates identity
Score > 26 indicates homology
QIAAEITK
1480 384.2163 1149.6271 1149.6254 1.43 1 17 0.087 +1Score > 38 indicates identity
Score > 19 indicates homology
QRSPAQLPPR + Deamidated (NQ)
1887 439.2495 1314.7267 1314.7143 9.40 1 17 0.077 +1Score > 40 indicates identity
Score > 19 indicates homology
IVLNIGSGAESVR + Deamidated (NQ)
1646 608.8137 1215.6128 1215.5983 12.0 1 17 0.34 +1Score > 41 indicates identity
Score > 25 indicates homology
ESLATLNSVPGE
851 437.2154 872.4162 872.4141 2.49 0 17 0.61 +1Score > 40 indicates identity
Score > 27 indicates homology
TPTDHFR
1619 602.8404 1203.6662 1203.6645 1.44 1 17 0.9 +1Score > 39 indicates identity
Score > 29 indicates homology
IISMQKGGNLK + Oxidation (M)
1793 637.8111 1273.6076 1273.5972 8.17 1 17 0.98 +1Score > 41 indicates identity
Score > 30 indicates homology
QPIMQGDTEQK
2268 499.3059 1494.8959 1494.8810 9.95 1 17 0.23 +1Score > 30 indicates identity
Score > 23 indicates homology
GILNFAPVVLEVPK
2279 749.9398 1497.8650 1497.8402 16.6 1 17 0.48 +1Score > 34 indicates identity
Score > 26 indicates homology
ISIAGRLVADLLQE + Deamidated (NQ)
1390 557.8304 1113.6462 1113.6393 6.21 0 17 0.9 +1Score > 39 indicates identity
Score > 29 indicates homology
IANVLLASNAK + Deamidated (NQ)
1724 620.3399 1238.6652 1238.6619 2.72 1 17 0.7 +1Score > 39 indicates identity
Score > 28 indicates homology
AHGQKVADALTK + Deamidated (NQ)
312 306.7086 611.4026 611.4119 -15.1 0 17 0.16 +1Score > 32 indicates identity
Score > 22 indicates homology
VIKPR
475 349.7331 697.4516 697.4486 4.34 0 17 0.98 +1Score > 31 indicates identity
Score > 30 indicates homology
AAKPALK
413 336.1917 670.3688 670.3650 5.78 0 17 0.79 +1Score > 36 indicates identity
Score > 29 indicates homology
AGDIAPK
1443 567.8116 1133.6086 1133.5937 13.2 0 17 0.8 +1Score > 41 indicates identity
Score > 29 indicates homology
LMDALMTAIR
2608 820.9205 1639.8264 1639.8279 -0.91 0 17 0.28 +1Score > 40 indicates identity
Score > 24 indicates homology
WIAYAQSLTSKPMK + Deamidated (NQ); Oxidation (M)
2372 772.4094 1542.8042 1542.8140 -6.35 0 17 0.28 +1Score > 40 indicates identity
Score > 24 indicates homology
LAIQNSQIASAIALE + 2 Deamidated (NQ)
790 424.7000 847.3854 847.3858 -0.40 0 17 0.94 +1Score > 41 indicates identity
Score > 29 indicates homology
NPDMISR + Oxidation (M)
3802 794.0992 2379.2758 2379.2885 -5.37 0 17 0.098 +1Score > 35 indicates identity
Score > 19 indicates homology
FGIGADGVLFLAKPLHTSLHMR
2326 758.3718 1514.7290 1514.7293 -0.18 1 17 0.94 +1Score > 41 indicates identity
Score > 29 indicates homology
VFSVFALFENDVE
3051 629.6771 1886.0095 1885.9997 5.20 0 17 0.3 +1Score > 38 indicates identity
Score > 24 indicates homology
TQLDTTTLPDLSAIAGLR + Deamidated (NQ)
962 308.5161 922.5265 922.5123 15.3 1 17 1 +1Score > 37 indicates identity
Score > 29 indicates homology
KYNLSVAK + Deamidated (NQ)
666 392.7702 783.5258 783.5331 -9.19 1 17 0.76 +1Score > 32 indicates identity
Score > 28 indicates homology
LGIVVKR
643 388.1914 774.3682 774.3733 -6.47 1 17 0.72 +1Score > 42 indicates identity
Score > 28 indicates homology
GGSRDQR
927 302.8478 905.5216 905.5083 14.7 1 17 0.79 +1Score > 38 indicates identity
Score > 28 indicates homology
IRFTVNR + Deamidated (NQ)
271 586.3885 585.3812 585.3737 12.8 0 17 0.38 +1Score > 39 indicates identity
Score > 25 indicates homology
ILDIL
693 399.2309 796.4472 796.4443 3.70 0 17 0.21 +1Score > 36 indicates identity
Score > 23 indicates homology
GAPAVTPGK
717 813.4374 812.4301 812.4392 -11.2 1 17 0.36 +1Score > 39 indicates identity
Score > 25 indicates homology
NPVVEVR + Deamidated (NQ)
1540 391.5694 1171.6864 1171.6673 16.3 1 17 0.33 +1Score > 37 indicates identity
Score > 25 indicates homology
QLQTGLSRLR + Deamidated (NQ)
281 300.6902 599.3658 599.3643 2.65 0 17 0.84 +1Score > 40 indicates identity
Score > 29 indicates homology
SVLGPK
211 503.3192 502.3119 502.3115 0.86 0 17 0.47 +1Score > 44 indicates identity
Score > 26 indicates homology
ASVVK
1671 408.5607 1222.6603 1222.6454 12.2 0 17 0.3 +1Score > 39 indicates identity
Score > 24 indicates homology
QISMIYPMLK
1717 619.8772 1237.7398 1237.7394 0.35 0 17 0.64 +1Score > 33 indicates identity
Score > 27 indicates homology
VAQALAVLTPVR + Deamidated (NQ)
1911 331.1999 1320.7705 1320.7765 -4.56 1 17 0.24 +1Score > 33 indicates identity
Score > 23 indicates homology
KPTSHILVEVAK
946 458.7716 915.5286 915.5250 3.96 1 17 0.92 +1Score > 40 indicates identity
Score > 29 indicates homology
VGVTTGRAR
1922 664.3893 1326.7640 1326.7659 -1.43 1 17 0.29 +1Score > 37 indicates identity
Score > 24 indicates homology
ISLDHLIFNKK
1511 388.2037 1161.5893 1161.5990 -8.35 1 17 0.74 +1Score > 42 indicates identity
Score > 28 indicates homology
TGTTSPSVQRK + Deamidated (NQ)
1920 663.4156 1324.8166 1324.8078 6.67 1 17 0.27 +1Score > 29 indicates identity
Score > 24 indicates homology
NVVVVAANGKILK + Deamidated (NQ)
852 437.2581 872.5016 872.4967 5.65 1 17 0.86 +1Score > 41 indicates identity
Score > 29 indicates homology
VLLGTENK
3120 969.5520 1937.0894 1937.0595 15.5 1 17 0.36 +1Score > 33 indicates identity
Score > 25 indicates homology
RAVAGNLSNILFIHADAR
1726 413.8959 1238.6659 1238.6507 12.3 1 17 0.51 +1Score > 39 indicates identity
Score > 26 indicates homology
QPIQLVEGLNE
551 365.2256 728.4366 728.4432 -9.02 0 17 0.85 +1Score > 41 indicates identity
Score > 29 indicates homology
ALADLVK
2258 747.8829 1493.7512 1493.7362 10.1 0 17 0.87 +1Score > 41 indicates identity
Score > 29 indicates homology
QVTLLGQNVNSYR + 3 Deamidated (NQ)
2740 854.9518 1707.8890 1707.8827 3.71 1 17 0.71 +1Score > 39 indicates identity
Score > 28 indicates homology
QELLLIIMGGVFVME + Deamidated (NQ); Oxidation (M)
1594 598.3866 1194.7586 1194.7448 11.6 0 17 0.19 +1Score > 27 indicates identity
Score > 22 indicates homology
IVVALGGNALLR
2477 529.2900 1584.8482 1584.8471 0.67 0 17 0.12 +1Score > 39 indicates identity
Score > 20 indicates homology
LSAQQLQTNVNVLR + 2 Deamidated (NQ)
1543 586.8562 1171.6978 1171.6812 14.2 1 17 0.99 +1Score > 35 indicates identity
Score > 29 indicates homology
LTLKLQTLNE
1610 601.3412 1200.6678 1200.6601 6.42 1 17 0.57 +1Score > 40 indicates identity
Score > 27 indicates homology
DLVENAISLVK + Deamidated (NQ)
298 301.6980 601.3814 601.3799 2.55 0 17 0.74 +1Score > 41 indicates identity
Score > 28 indicates homology
SVLVGK
1575 396.8766 1187.6080 1187.5894 15.6 1 17 0.41 +1Score > 42 indicates identity
Score > 25 indicates homology
QQIQRTQQR + 3 Deamidated (NQ)
3293 1030.5316 2059.0486 2059.0545 -2.85 1 17 0.69 +1Score > 39 indicates identity
Score > 28 indicates homology
IVSSQINSGNALSADVQKAR + 2 Deamidated (NQ)
471 348.1715 694.3284 694.3286 -0.19 1 17 0.75 +1Score > 41 indicates identity
Score > 28 indicates homology
IYEDR
1243 518.2980 1034.5814 1034.6012 -19.1 1 17 0.36 +1Score > 38 indicates identity
Score > 25 indicates homology
IEIYIVGTK
3612 746.4070 2236.1992 2236.2150 -7.09 1 17 0.32 +1Score > 36 indicates identity
Score > 24 indicates homology
MFLQARAALVTGGALYIVGNR + Oxidation (M)
1104 325.1949 972.5629 972.5604 2.58 1 17 0.41 +1Score > 39 indicates identity
Score > 25 indicates homology
KLQQLSQK + Deamidated (NQ)
2036 693.8922 1385.7698 1385.7765 -4.83 1 17 0.42 +1Score > 38 indicates identity
Score > 25 indicates homology
LLAAQKQAIATQK + 3 Deamidated (NQ)
3857 605.3223 2417.2601 2417.2660 -2.43 0 17 0.079 +1Score > 37 indicates identity
Score > 18 indicates homology
LSAMMVTSFILISVLALGLMYE + Oxidation (M)
1331 361.8495 1082.5267 1082.5067 18.5 0 17 0.79 +1Score > 40 indicates identity
Score > 28 indicates homology
MNVFNTDVK + Oxidation (M)
1598 399.8961 1196.6665 1196.6877 -17.7 0 17 0.86 +1Score > 38 indicates identity
Score > 28 indicates homology
AVLSAIGNPSLR
1893 439.8843 1316.6311 1316.6395 -6.37 0 17 0.28 +1Score > 41 indicates identity
Score > 24 indicates homology
VPVIAGCGSNDTK
1780 633.8815 1265.7484 1265.7343 11.2 1 17 0.6 +1Score > 33 indicates identity
Score > 27 indicates homology
LQLQELGPVIR + Deamidated (NQ)
1853 648.8711 1295.7276 1295.7193 6.48 1 17 0.74 +1Score > 37 indicates identity
Score > 28 indicates homology
MSMLSTLLLKK + 2 Oxidation (M)
1006 314.8369 941.4889 941.5042 -16.3 1 17 0.53 +1Score > 40 indicates identity
Score > 26 indicates homology
ASRLPGANR + Deamidated (NQ)
2400 519.3011 1554.8815 1554.8617 12.7 0 17 0.43 +1Score > 36 indicates identity
Score > 25 indicates homology
VTQLLSLLANVQQE
2953 609.3014 1824.8824 1824.8754 3.81 1 17 0.66 +1Score > 40 indicates identity
Score > 27 indicates homology
QNRPYNHPELQGITR + 3 Deamidated (NQ)
2824 879.9953 1757.9760 1757.9411 19.9 1 16 0.82 +1Score > 36 indicates identity
Score > 28 indicates homology
QLNILTQTVSILEQR + 3 Deamidated (NQ)
1466 571.8625 1141.7104 1141.7070 2.98 0 16 0.76 +1Score > 30 indicates identity
Score > 28 indicates homology
ISIQVLQTLK
2302 755.9500 1509.8854 1509.8654 13.3 0 16 0.96 +1Score > 33 indicates identity
Score > 29 indicates homology
LIVLTDTPGILLNE
894 447.2434 892.4722 892.4767 -4.94 1 16 0.96 +1Score > 41 indicates identity
Score > 29 indicates homology
DIFKNTR
2413 781.4036 1560.7926 1560.8181 -16.3 1 16 0.81 +1Score > 41 indicates identity
Score > 28 indicates homology
LSVMAVGGELVDISR + Oxidation (M)
2422 782.4335 1562.8524 1562.8304 14.1 1 16 0.77 +1Score > 37 indicates identity
Score > 28 indicates homology
VDLGSKALDDVLYR
513 354.7064 707.3982 707.3966 2.30 0 16 0.4 +1Score > 39 indicates identity
Score > 25 indicates homology
ISVFSR
2005 456.5699 1366.6879 1366.7092 -15.6 0 16 0.93 +1Score > 40 indicates identity
Score > 29 indicates homology
GVPTGAVAINPANGK + 2 Deamidated (NQ)
1019 316.1801 945.5185 945.5131 5.67 0 16 0.93 +1Score > 41 indicates identity
Score > 29 indicates homology
AGQSSVLVGK + Deamidated (NQ)
1124 494.2783 986.5420 986.5509 -8.96 0 16 0.78 +1Score > 42 indicates identity
Score > 28 indicates homology
VVATAAAATGR
2937 908.4622 1814.9098 1814.8911 10.3 1 16 0.09 +1Score > 40 indicates identity
Score > 18 indicates homology
NNRFIGQAAIGGEPQDK + Deamidated (NQ)
793 425.7155 849.4164 849.4232 -7.97 1 16 0.66 +1Score > 41 indicates identity
Score > 27 indicates homology
EAADFVAK
1607 400.8647 1199.5723 1199.5935 -17.7 1 16 0.64 +1Score > 40 indicates identity
Score > 27 indicates homology
KFDFSNLSSR
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