MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita6 sp
MS data file : PRT1270_T-BRSC_6_20250714122635.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:38:56 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,869

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 501–600 (out of 3786)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
1170 503.2974 1004.5802 1004.5654 14.7 0 19 0.81 +1Score > 37 indicates identity
Score > 31 indicates homology		 AIDAYIALR
1497 579.7746 1157.5346 1157.5452 -9.11 1 19 0.98 +1Score > 40 indicates identity
Score > 31 indicates homology		 VAPDLTAEDVE
1156 499.7990 997.5834 997.5808 2.69 1 19 0.19 +1Score > 38 indicates identity
Score > 24 indicates homology		 LLQSIPEAK
3708 1152.0691 2302.1236 2302.1176 2.62 1 19 0.12 +1Score > 39 indicates identity
Score > 22 indicates homology		 SVTIDKDSVNAVLLDTDPQDR + 2 Deamidated (NQ)
236 531.2910 530.2837 530.2813 4.67 1 19 0.98 +1Score > 46 indicates identity
Score > 31 indicates homology		 EVGAR
681 395.7212 789.4278 789.4232 5.87 0 19 0.57 +1Score > 41 indicates identity
Score > 29 indicates homology		 VISAQSGK + Deamidated (NQ)
1772 421.5728 1261.6966 1261.6918 3.79 1 19 0.69 +1Score > 39 indicates identity
Score > 30 indicates homology		 GLSVEFALLADK
1335 542.7901 1083.5656 1083.5601 5.16 0 19 0.062 +1Score > 40 indicates identity
Score > 19 indicates homology		 GGSYYPITVK
299 301.6984 601.3822 601.3911 -14.8 1 19 0.15 +1Score > 41 indicates identity
Score > 23 indicates homology		 ISVKR
1877 437.2217 1308.6433 1308.6561 -9.82 1 19 0.26 +1Score > 41 indicates identity
Score > 26 indicates homology		 QNITETSFVLR + 2 Deamidated (NQ)
1410 374.5374 1120.5904 1120.5798 9.45 1 19 0.99 +1Score > 40 indicates identity
Score > 31 indicates homology		 KTIMLTGDNK + Deamidated (NQ)
1785 635.3817 1268.7488 1268.7704 -17.0 0 19 0.81 +1Score > 35 indicates identity
Score > 30 indicates homology		 LIVIDLLSVQR + Deamidated (NQ)
2180 726.3380 1450.6614 1450.6463 10.4 1 19 0.96 +1Score > 40 indicates identity
Score > 31 indicates homology		 SEGFILANPTSNAE + 2 Deamidated (NQ)
1728 414.2371 1239.6895 1239.7122 -18.3 1 19 0.77 +1Score > 38 indicates identity
Score > 30 indicates homology		 VGRCLGAPIAVK
703 401.2144 800.4142 800.4253 -13.8 1 19 0.5 +1Score > 42 indicates identity
Score > 28 indicates homology		 QDGRGIR
2402 778.4544 1554.8942 1554.8729 13.7 1 19 0.71 +1Score > 35 indicates identity
Score > 30 indicates homology		 QQLKSAGSVLISAPR + Deamidated (NQ)
3209 670.7066 2009.0980 2009.1381 -20.0 1 19 0.15 +1Score > 36 indicates identity
Score > 23 indicates homology		 TSLGTINHSLLSLAALRSR
237 531.3182 530.3109 530.3176 -12.6 0 19 0.72 +1Score > 47 indicates identity
Score > 30 indicates homology		 AITAR
2053 695.8816 1389.7486 1389.7616 -9.31 0 19 0.31 +1Score > 40 indicates identity
Score > 26 indicates homology		 LQYAGLLSQLQR + Deamidated (NQ)
3218 1006.4937 2010.9728 2010.9660 3.40 0 19 0.17 +1Score > 40 indicates identity
Score > 24 indicates homology		 QLTHQQIYPHFADGSNR
887 444.2661 886.5176 886.5124 5.96 1 19 0.86 +1Score > 40 indicates identity
Score > 31 indicates homology		 VDVNIKAK + Deamidated (NQ)
2741 854.9531 1707.8916 1707.8679 13.9 1 19 0.71 +1Score > 39 indicates identity
Score > 30 indicates homology		 QLQLVKDVLNHAAQE + 3 Deamidated (NQ)
594 379.2510 756.4874 756.4858 2.21 0 19 0.99 +1Score > 39 indicates identity
Score > 31 indicates homology		 VIVTGLR
1020 316.4915 946.4527 946.4607 -8.49 1 19 0.67 +1Score > 42 indicates identity
Score > 29 indicates homology		 ANEVTLNGK + 2 Deamidated (NQ)
611 381.2068 760.3990 760.3967 3.15 0 19 0.6 +1Score > 43 indicates identity
Score > 29 indicates homology		 INSADLK + Deamidated (NQ)
1527 584.8431 1167.6716 1167.6863 -12.5 1 19 0.93 +1Score > 37 indicates identity
Score > 31 indicates homology		 QEGLLLPSALK
1406 560.8057 1119.5968 1119.5812 14.0 0 19 0.53 +1Score > 41 indicates identity
Score > 28 indicates homology		 LISDPYGNLK + Deamidated (NQ)
1602 600.3370 1198.6594 1198.6418 14.7 1 19 0.49 +1Score > 40 indicates identity
Score > 28 indicates homology		 DVLALARDGNR
1932 444.5923 1330.7551 1330.7609 -4.36 1 19 0.25 +1Score > 36 indicates identity
Score > 25 indicates homology		 FANVLKDLGVQK
2219 491.9370 1472.7892 1472.7657 15.9 0 19 0.51 +1Score > 40 indicates identity
Score > 28 indicates homology		 AGMKPLQASLQGVR + 2 Deamidated (NQ); Oxidation (M)
1283 529.7916 1057.5686 1057.5655 2.99 1 19 0.86 +1Score > 42 indicates identity
Score > 30 indicates homology		 QQLNEILAK + 2 Deamidated (NQ)
570 370.7258 739.4370 739.4341 4.03 0 19 0.13 +1Score > 35 indicates identity
Score > 22 indicates homology		 AVAVAGPR
442 340.6806 679.3466 679.3442 3.61 0 19 0.66 +1Score > 40 indicates identity
Score > 29 indicates homology		 FPNFR
2461 790.4498 1578.8850 1578.8729 7.68 1 19 0.39 +1Score > 36 indicates identity
Score > 27 indicates homology		 ELQANVVHLSLLSR + Deamidated (NQ)
454 344.2247 686.4348 686.4439 -13.2 1 18 0.62 +1Score > 40 indicates identity
Score > 29 indicates homology		 SVKVVR
786 423.7282 845.4418 845.4429 -1.26 0 18 0.55 +1Score > 42 indicates identity
Score > 28 indicates homology		 LSVGPMAR + Oxidation (M)
3465 721.0508 2160.1306 2160.1063 11.3 0 18 0.67 +1Score > 38 indicates identity
Score > 29 indicates homology		 TDPYVTPGSVINNLSINTVR + Deamidated (NQ)
1500 387.2041 1158.5905 1158.5993 -7.62 1 18 0.5 +1Score > 42 indicates identity
Score > 28 indicates homology		 GKQQDTVLGGR + Deamidated (NQ)
1850 432.9158 1295.7256 1295.7193 4.88 1 18 0.66 +1Score > 37 indicates identity
Score > 29 indicates homology		 MSMLSTLLLKK + 2 Oxidation (M)
2641 829.9388 1657.8630 1657.8410 13.3 0 18 0.66 +1Score > 40 indicates identity
Score > 29 indicates homology		 SLIQNGADINVIASIE + 2 Deamidated (NQ)
3324 696.3436 2086.0090 2085.9967 5.89 1 18 0.84 +1Score > 39 indicates identity
Score > 30 indicates homology		 RDASWQHLTALASTLTADE + Deamidated (NQ)
3524 730.3752 2188.1038 2188.1198 -7.32 1 18 0.13 +1Score > 39 indicates identity
Score > 22 indicates homology		 INAAHGFSLIQVDNTKVTMK + 2 Deamidated (NQ)
327 308.6872 615.3598 615.3592 1.12 0 18 0.56 +1Score > 46 indicates identity
Score > 28 indicates homology		 ADVIAK
967 463.2815 924.5484 924.5392 9.95 0 18 0.097 +1Score > 36 indicates identity
Score > 21 indicates homology		 GLQIAAVPR + Deamidated (NQ)
1010 471.7740 941.5334 941.5294 4.30 0 18 0.85 +1Score > 39 indicates identity
Score > 30 indicates homology		 IQNAILDR
2052 695.8795 1389.7444 1389.7503 -4.24 1 18 0.74 +1Score > 40 indicates identity
Score > 29 indicates homology		 YIAKNIVAADLAE
2892 897.9699 1793.9252 1793.9523 -15.1 1 18 0.29 +1Score > 39 indicates identity
Score > 25 indicates homology		 QQDIPVENVLALGGIAR + 2 Deamidated (NQ)
3374 706.0299 2115.0679 2115.0670 0.40 0 18 0.7 +1Score > 39 indicates identity
Score > 29 indicates homology		 AMIDQVAAVGILQSWLDQR + 2 Deamidated (NQ)
2737 570.2945 1707.8617 1707.8389 13.3 0 18 0.29 +1Score > 40 indicates identity
Score > 25 indicates homology		 TSFQMLPISNEPISK + Deamidated (NQ); Oxidation (M)
620 382.7327 763.4508 763.4480 3.79 0 18 0.51 +1Score > 35 indicates identity
Score > 28 indicates homology		 IINYIK + Deamidated (NQ)
1912 331.2001 1320.7713 1320.7666 3.54 0 18 0.95 +1Score > 32 indicates identity
Score > 30 indicates homology		 LLQTAHLWLAR
2183 485.9579 1454.8519 1454.8318 13.8 1 18 0.11 +1Score > 34 indicates identity
Score > 21 indicates homology		 TAVVVGAGRSGLAAAR
841 434.2581 866.5016 866.4974 4.93 1 18 0.28 +1Score > 36 indicates identity
Score > 25 indicates homology		 HEILISR
1015 473.2493 944.4840 944.4927 -9.15 0 18 0.84 +1Score > 42 indicates identity
Score > 30 indicates homology		 NNTQLVQK + Deamidated (NQ)
297 602.3638 601.3565 601.3548 2.95 0 18 0.69 +1Score > 43 indicates identity
Score > 29 indicates homology		 GVASLR
2261 747.8837 1493.7528 1493.7699 -11.4 1 18 0.86 +1Score > 41 indicates identity
Score > 30 indicates homology		 LNTLSPANGARHSR + Deamidated (NQ)
985 465.2776 928.5406 928.5342 6.98 1 18 0.27 +1Score > 41 indicates identity
Score > 25 indicates homology		 VLLEAGATR
1541 586.8512 1171.6878 1171.6812 5.68 1 18 0.91 +1Score > 36 indicates identity
Score > 30 indicates homology		 VNNIKITQIK + 2 Deamidated (NQ)
2626 825.9310 1649.8474 1649.8624 -9.09 0 18 0.61 +1Score > 40 indicates identity
Score > 28 indicates homology		 ITTVQAAIDFIQSSR + Deamidated (NQ)
250 558.3638 557.3565 557.3537 5.10 0 18 0.76 +1Score > 40 indicates identity
Score > 29 indicates homology		 AGAVLK
1394 558.3033 1114.5920 1114.5982 -5.52 0 18 0.69 +1Score > 41 indicates identity
Score > 29 indicates homology		 ANILNSDQIK
831 430.7494 859.4842 859.4837 0.63 1 18 0.86 +1Score > 41 indicates identity
Score > 30 indicates homology		 LLMKDPK + Oxidation (M)
2051 695.8285 1389.6424 1389.6372 3.78 1 18 0.33 +1Score > 40 indicates identity
Score > 26 indicates homology		 ADSLTAGGLDARDE
1876 654.8464 1307.6782 1307.6721 4.70 1 18 0.87 +1Score > 40 indicates identity
Score > 30 indicates homology		 LYELIDSNSVR
2490 531.2798 1590.8176 1590.8293 -7.39 0 18 0.46 +1Score > 40 indicates identity
Score > 27 indicates homology		 YSLDSYLISVYIR
1735 415.5678 1243.6816 1243.7023 -16.7 1 18 0.84 +1Score > 40 indicates identity
Score > 30 indicates homology		 IAIITNNNKLK + 3 Deamidated (NQ)
417 336.2268 670.4390 670.4377 1.94 0 18 0.34 +1Score > 37 indicates identity
Score > 26 indicates homology		 VAGLAIK
1401 560.7929 1119.5712 1119.5924 -18.9 1 18 0.31 +1Score > 42 indicates identity
Score > 25 indicates homology		 QFLNEILSR + Deamidated (NQ)
2544 538.6297 1612.8673 1612.8395 17.2 1 18 0.44 +1Score > 39 indicates identity
Score > 27 indicates homology		 CPYLQAILHETLR
1686 410.5760 1228.7062 1228.7139 -6.32 1 18 0.7 +1Score > 38 indicates identity
Score > 29 indicates homology		 QTQVTVQKIGK
3156 986.5366 1971.0586 1971.0425 8.18 1 18 0.18 +1Score > 37 indicates identity
Score > 23 indicates homology		 ADVLSDTRALLSALDFHK
1247 519.2740 1036.5334 1036.5488 -14.8 1 18 0.15 +1Score > 40 indicates identity
Score > 22 indicates homology		 LAAAFKMNR + Oxidation (M)
2637 828.9891 1655.9636 1655.9458 10.8 1 18 0.14 +1Score > 32 indicates identity
Score > 22 indicates homology		 ILSTRSQIVGIDLIE
1684 615.3379 1228.6612 1228.6663 -4.13 1 18 0.42 +1Score > 41 indicates identity
Score > 27 indicates homology		 LVDGAQVTGELK
223 516.3042 515.2969 515.2955 2.79 0 18 0.38 +1Score > 47 indicates identity
Score > 26 indicates homology		 ALAQL + Deamidated (NQ)
729 409.7175 817.4204 817.4293 -10.9 1 18 0.44 +1Score > 44 indicates identity
Score > 27 indicates homology		 KNSNNIK + Deamidated (NQ)
1638 405.5731 1213.6975 1213.7104 -10.7 1 18 0.79 +1Score > 38 indicates identity
Score > 29 indicates homology		 IDLQKMLLPK + Oxidation (M)
1935 667.8631 1333.7116 1333.6990 9.49 1 18 0.22 +1Score > 40 indicates identity
Score > 24 indicates homology		 SFLVNLDRNQK + Deamidated (NQ)
897 447.7387 893.4628 893.4607 2.43 0 18 0.6 +1Score > 41 indicates identity
Score > 28 indicates homology		 VTGLGQYR + Deamidated (NQ)
519 358.7016 715.3886 715.3752 18.8 0 18 0.46 +1Score > 44 indicates identity
Score > 27 indicates homology		 SPALISE
563 734.3674 733.3601 733.3606 -0.68 1 18 0.47 +1Score > 42 indicates identity
Score > 27 indicates homology		 DTTLER
3141 980.0217 1958.0288 1957.9938 17.9 0 18 0.22 +1Score > 38 indicates identity
Score > 24 indicates homology		 FLSQPFFVAAQFTGVDGK
2891 897.9683 1793.9220 1793.9523 -16.9 1 18 0.27 +1Score > 40 indicates identity
Score > 25 indicates homology		 QQDIPVENVLALGGIAR + 2 Deamidated (NQ)
914 300.8195 899.4367 899.4501 -15.0 0 18 1 +1Score > 39 indicates identity
Score > 30 indicates homology		 DAWGPGVAK
1931 666.3821 1330.7496 1330.7570 -5.56 0 18 0.41 +1Score > 38 indicates identity
Score > 26 indicates homology		 LLMVSVVNFVPL + Deamidated (NQ)
546 364.7044 727.3942 727.3977 -4.69 1 18 0.96 +1Score > 38 indicates identity
Score > 30 indicates homology		 KSSAAHK
1252 347.8551 1040.5435 1040.5251 17.7 1 18 0.68 +1Score > 39 indicates identity
Score > 29 indicates homology		 DLPGQAEVGR
1123 494.2667 986.5188 986.5032 15.8 1 18 0.82 +1Score > 42 indicates identity
Score > 29 indicates homology		 LQQAQAEAK + Deamidated (NQ)
608 381.2009 760.3872 760.3902 -3.82 0 18 0.36 +1Score > 43 indicates identity
Score > 26 indicates homology		 KPMDVR + Oxidation (M)
649 388.7092 775.4038 775.4076 -4.79 0 18 0.98 +1Score > 42 indicates identity
Score > 30 indicates homology		 QSSNVIK + Deamidated (NQ)
1021 316.4919 946.4539 946.4720 -19.1 1 18 0.73 +1Score > 42 indicates identity
Score > 29 indicates homology		 GENIGTTVR + Deamidated (NQ)
502 352.2220 702.4294 702.4276 2.66 0 18 0.86 +1Score > 38 indicates identity
Score > 30 indicates homology		 ITTAGIK
1297 534.2437 1066.4728 1066.4567 15.2 1 18 0.38 +1Score > 40 indicates identity
Score > 26 indicates homology		 FENNTVNAR + 3 Deamidated (NQ)
2171 722.8694 1443.7242 1443.7358 -7.99 1 18 0.46 +1Score > 41 indicates identity
Score > 27 indicates homology		 WDVNIGELAGITR + Deamidated (NQ)
2515 536.3085 1605.9037 1605.9164 -7.91 1 18 0.18 +1Score > 35 indicates identity
Score > 23 indicates homology		 IVSAVMLALIELYR + Oxidation (M)
1710 413.2139 1236.6199 1236.6271 -5.87 1 18 0.58 +1Score > 41 indicates identity
Score > 28 indicates homology		 SLVQLSTMETK + Deamidated (NQ)
313 307.1818 612.3490 612.3595 -17.1 1 18 0.39 +1Score > 38 indicates identity
Score > 26 indicates homology		 IEVPR
2554 807.9299 1613.8452 1613.8373 4.94 1 18 0.2 +1Score > 40 indicates identity
Score > 23 indicates homology		 QAADDPLNTVSSIKR
1766 421.2318 1260.6736 1260.6979 -19.3 1 18 0.89 +1Score > 41 indicates identity
Score > 30 indicates homology		 FPFSRPELLR
1475 574.7845 1147.5544 1147.5656 -9.69 1 18 0.98 +1Score > 42 indicates identity
Score > 30 indicates homology		 EQATVVCVSR
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