MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita6 sp
MS data file : PRT1270_T-BRSC_6_20250714122635.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:38:56 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,869

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 401–500 (out of 3786)


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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
402 334.7089 667.4032 667.4017 2.31 0 20 0.27 +1Score > 30 indicates identity
Score > 27 indicates homology
VPIPSR
1589 597.3406 1192.6666 1192.6676 -0.83 1 20 0.17 +1Score > 38 indicates identity
Score > 25 indicates homology
GALEVRPPQAR
1729 414.2466 1239.7180 1239.7339 -12.8 1 20 0.17 +1Score > 37 indicates identity
Score > 25 indicates homology
HPSKLLAYALK
2380 774.3989 1546.7832 1546.8103 -17.5 1 20 0.56 +1Score > 41 indicates identity
Score > 30 indicates homology
EAAAPGAAPAAAAAAPAAK
862 438.7521 875.4896 875.4865 3.62 1 20 0.38 +1Score > 40 indicates identity
Score > 29 indicates homology
LFAEQIR
595 379.2512 756.4878 756.4858 2.75 0 20 0.51 +1Score > 39 indicates identity
Score > 30 indicates homology
VIVASIR
365 322.1886 642.3626 642.3701 -11.5 0 20 0.54 +1Score > 35 indicates identity
Score > 30 indicates homology
IAPGSAK
1185 506.3130 1010.6114 1010.6012 10.2 0 20 0.46 +1Score > 34 indicates identity
Score > 29 indicates homology
INALQLIPK + 2 Deamidated (NQ)
1654 610.3046 1218.5946 1218.5961 -1.22 1 20 0.9 +1Score > 42 indicates identity
Score > 32 indicates homology
AVMDVANGRMR
336 312.1739 622.3332 622.3439 -17.0 0 20 0.079 +1Score > 41 indicates identity
Score > 22 indicates homology
ITFSR
2743 854.9540 1707.8934 1707.8679 15.0 1 20 0.71 +1Score > 39 indicates identity
Score > 31 indicates homology
QLQLVKDVLNHAAQE + 3 Deamidated (NQ)
1431 566.2869 1130.5592 1130.5642 -4.35 0 20 0.61 +1Score > 41 indicates identity
Score > 31 indicates homology
VVIQDPMGQK + Deamidated (NQ); Oxidation (M)
1379 554.7884 1107.5622 1107.5812 -17.1 0 20 0.49 +1Score > 41 indicates identity
Score > 30 indicates homology
YNTLIDLQK + Deamidated (NQ)
357 316.7033 631.3920 631.3905 2.50 0 20 0.69 +1Score > 31 indicates identity
Score > 31 indicates homology
ITTIGK
1433 566.2872 1130.5598 1130.5642 -3.82 0 20 0.99 +1Score > 42 indicates identity
Score > 33 indicates homology
VVIQDPMGQK + Deamidated (NQ); Oxidation (M)
984 465.2774 928.5402 928.5454 -5.53 1 20 0.87 +1Score > 41 indicates identity
Score > 32 indicates homology
IINSARQK
528 360.1838 718.3530 718.3610 -11.0 0 20 0.47 +1Score > 43 indicates identity
Score > 29 indicates homology
TGIDASR
2250 496.5933 1486.7581 1486.7793 -14.3 1 20 0.46 +1Score > 41 indicates identity
Score > 29 indicates homology
LAQWFDAARRPR + Deamidated (NQ)
1725 620.3400 1238.6654 1238.6805 -12.1 1 20 0.59 +1Score > 39 indicates identity
Score > 30 indicates homology
ALHQQICKIK + Deamidated (NQ)
561 366.7177 731.4208 731.4290 -11.1 1 20 0.99 +1Score > 41 indicates identity
Score > 33 indicates homology
TGTARVK
1579 594.8350 1187.6554 1187.6510 3.77 1 20 0.78 +1Score > 40 indicates identity
Score > 32 indicates homology
LTKSQLQQNK + Deamidated (NQ)
2795 866.9467 1731.8788 1731.8890 -5.88 0 20 0.61 +1Score > 40 indicates identity
Score > 31 indicates homology
LVQAVIAGGSSSLINSSE + Deamidated (NQ)
1207 510.2827 1018.5508 1018.5335 17.1 0 20 0.62 +1Score > 41 indicates identity
Score > 30 indicates homology
AGFNLLLAAE + Deamidated (NQ)
599 380.2356 758.4566 758.4538 3.76 1 20 0.89 +1Score > 40 indicates identity
Score > 32 indicates homology
TLVIGEK
859 438.2268 874.4390 874.4508 -13.5 1 20 0.084 +1Score > 43 indicates identity
Score > 22 indicates homology
QSAARLTE
932 455.7573 909.5000 909.5106 -11.6 0 20 0.26 +1Score > 38 indicates identity
Score > 27 indicates homology
MLPGVLHK + Oxidation (M)
834 431.2388 860.4630 860.4603 3.16 0 20 0.85 +1Score > 42 indicates identity
Score > 32 indicates homology
TATIVNNK + Deamidated (NQ)
886 444.2595 886.5044 886.4872 19.5 0 20 0.63 +1Score > 42 indicates identity
Score > 31 indicates homology
LAQSQALR + Deamidated (NQ)
420 336.7108 671.4070 671.4078 -1.18 1 20 0.87 +1Score > 42 indicates identity
Score > 32 indicates homology
LAAGRGK
2375 515.2872 1542.8398 1542.8617 -14.2 0 20 0.56 +1Score > 39 indicates identity
Score > 30 indicates homology
SGATLNQLLVQSAIK + Deamidated (NQ)
900 447.7388 893.4630 893.4528 11.4 1 20 0.68 +1Score > 41 indicates identity
Score > 31 indicates homology
TGVEMTLK + Oxidation (M)
800 426.7138 851.4130 851.4025 12.4 1 20 0.074 +1Score > 41 indicates identity
Score > 21 indicates homology
TAAQFEGK + Deamidated (NQ)
785 423.7274 845.4402 845.4317 10.1 1 20 0.56 +1Score > 43 indicates identity
Score > 30 indicates homology
IPDDKMK
901 447.7391 893.4636 893.4607 3.34 1 20 0.25 +1Score > 41 indicates identity
Score > 27 indicates homology
LSGELYGR
1076 322.1756 963.5050 963.5025 2.55 1 20 0.6 +1Score > 41 indicates identity
Score > 30 indicates homology
SFAQAAIEK
1991 455.2211 1362.6415 1362.6561 -10.8 1 20 0.46 +1Score > 41 indicates identity
Score > 29 indicates homology
SGLNMQDRANLK + Deamidated (NQ); Oxidation (M)
1412 374.8619 1121.5639 1121.5499 12.5 1 20 0.54 +1Score > 41 indicates identity
Score > 30 indicates homology
TGSQANKLMR + Deamidated (NQ); Oxidation (M)
2116 708.3635 1414.7124 1414.7245 -8.50 0 20 0.82 +1Score > 40 indicates identity
Score > 32 indicates homology
LIWGVYPANEPR + Deamidated (NQ)
1405 560.8030 1119.5914 1119.6036 -10.9 1 20 0.5 +1Score > 41 indicates identity
Score > 29 indicates homology
KGNSYPVISR
1536 586.8329 1171.6512 1171.6448 5.49 1 20 0.5 +1Score > 40 indicates identity
Score > 29 indicates homology
QLGLINEAVSK + Deamidated (NQ)
2360 512.6511 1534.9315 1534.9017 19.4 1 20 0.28 +1Score > 28 indicates identity
Score > 27 indicates homology
MNLILVKGQIPGPR
525 360.1780 718.3414 718.3432 -2.44 0 20 0.94 +1Score > 43 indicates identity
Score > 32 indicates homology
APMSTGR
1601 600.3364 1198.6582 1198.6418 13.7 1 20 0.52 +1Score > 40 indicates identity
Score > 30 indicates homology
DVLALARDGNR
1603 600.3387 1198.6628 1198.6418 17.5 1 20 0.96 +1Score > 40 indicates identity
Score > 32 indicates homology
DVLALARDGNR
1114 491.7408 981.4670 981.4556 11.7 1 20 0.42 +1Score > 41 indicates identity
Score > 29 indicates homology
WEQYVTR + Deamidated (NQ)
2403 519.6351 1555.8835 1555.8756 5.07 0 20 0.36 +1Score > 36 indicates identity
Score > 28 indicates homology
LMNVPIAIVSLGASR + Oxidation (M)
1666 611.8103 1221.6060 1221.6023 3.06 1 20 0.51 +1Score > 41 indicates identity
Score > 29 indicates homology
QKGLSSAMLDR + Deamidated (NQ); Oxidation (M)
1461 571.8091 1141.6036 1141.5866 14.9 1 20 0.7 +1Score > 40 indicates identity
Score > 31 indicates homology
IDQIKINPAE + 2 Deamidated (NQ)
2997 619.6636 1855.9690 1855.9891 -10.8 0 20 0.1 +1Score > 39 indicates identity
Score > 22 indicates homology
VLTSDSNIVPVDISGIAR + Deamidated (NQ)
1089 324.8388 971.4946 971.5036 -9.28 1 20 0.9 +1Score > 40 indicates identity
Score > 32 indicates homology
AIEQVGGAAR + Deamidated (NQ)
1404 560.7968 1119.5790 1119.5924 -11.9 1 20 0.49 +1Score > 41 indicates identity
Score > 29 indicates homology
GLQLFSVARE + Deamidated (NQ)
2543 807.4170 1612.8194 1612.8308 -7.05 1 20 0.74 +1Score > 40 indicates identity
Score > 31 indicates homology
VTGALESGANAVLVSPE
867 440.2525 878.4904 878.4974 -7.90 1 20 0.82 +1Score > 38 indicates identity
Score > 31 indicates homology
AKFSTGIR
1326 540.3030 1078.5914 1078.6022 -10.00 1 20 0.9 +1Score > 38 indicates identity
Score > 32 indicates homology
IISQQFKSK + Deamidated (NQ)
1834 645.3408 1288.6670 1288.6623 3.70 1 20 0.81 +1Score > 42 indicates identity
Score > 31 indicates homology
ALASEIQTSVDR
1193 1015.6124 1014.6051 1014.5896 15.3 0 20 0.46 +1Score > 37 indicates identity
Score > 29 indicates homology
SVIMALIPR + Oxidation (M)
699 400.7182 799.4218 799.4188 3.80 0 20 0.73 +1Score > 39 indicates identity
Score > 31 indicates homology
VNSVQPR + Deamidated (NQ)
2459 789.4291 1576.8436 1576.8395 2.63 1 20 0.24 +1Score > 39 indicates identity
Score > 26 indicates homology
FLGNLMQLLNNKR + Deamidated (NQ); Oxidation (M)
806 854.4744 853.4671 853.4657 1.63 0 20 0.21 +1Score > 37 indicates identity
Score > 25 indicates homology
APAAAPAASK
2181 484.5867 1450.7383 1450.7589 -14.2 1 20 0.23 +1Score > 41 indicates identity
Score > 26 indicates homology
LSTMLTTNAILKE + Deamidated (NQ); Oxidation (M)
2349 510.6246 1528.8520 1528.8249 17.7 0 20 0.14 +1Score > 37 indicates identity
Score > 23 indicates homology
FNHQLTDLTSIIK
1235 517.7535 1033.4924 1033.4928 -0.31 0 20 0.56 +1Score > 42 indicates identity
Score > 30 indicates homology
TLSDSGLNVE
711 401.7217 801.4288 801.4344 -6.99 1 20 0.96 +1Score > 43 indicates identity
Score > 32 indicates homology
LPKDSSR
3289 686.9671 2057.8795 2057.9187 -19.0 1 20 0.17 +1Score > 36 indicates identity
Score > 24 indicates homology
DLYNWTMDEIVSCAGIR + Oxidation (M)
1580 594.8380 1187.6614 1187.6510 8.82 1 19 0.68 +1Score > 40 indicates identity
Score > 30 indicates homology
LTKSQLQQNK + Deamidated (NQ)
1238 345.8500 1034.5282 1034.5145 13.2 1 19 0.29 +1Score > 41 indicates identity
Score > 27 indicates homology
GNEFGVVTGR
2353 766.9410 1531.8674 1531.8947 -17.8 1 19 0.11 +1Score > 35 indicates identity
Score > 22 indicates homology
GSVVLLNRAPTLHR
2470 791.3878 1580.7610 1580.7504 6.71 1 19 0.55 +1Score > 41 indicates identity
Score > 29 indicates homology
SRLQSIMGYPVQGE + Deamidated (NQ); Oxidation (M)
989 465.7684 929.5222 929.5042 19.4 1 19 1 +1Score > 42 indicates identity
Score > 32 indicates homology
IASARNAAR + Deamidated (NQ)
3294 687.3573 2059.0501 2059.0143 17.4 1 19 0.69 +1Score > 39 indicates identity
Score > 30 indicates homology
TTDLQTTLQLSMKAIQHE + 2 Deamidated (NQ)
2464 527.3040 1578.8902 1578.9093 -12.1 1 19 0.32 +1Score > 35 indicates identity
Score > 27 indicates homology
VSELVNLPAVGVNLR
1239 518.2791 1034.5436 1034.5430 0.63 1 19 0.48 +1Score > 41 indicates identity
Score > 29 indicates homology
LMNKQSGLK + Deamidated (NQ); Oxidation (M)
3347 1051.0117 2100.0088 2100.0348 -12.4 1 19 0.2 +1Score > 39 indicates identity
Score > 25 indicates homology
GSRSVDHGYLQQIAQVADR + Deamidated (NQ)
3941 837.0775 2508.2107 2508.1703 16.1 0 19 0.61 +1Score > 38 indicates identity
Score > 30 indicates homology
ATCTTHHDSPDADLITANLLWR + Deamidated (NQ)
1894 659.8907 1317.7668 1317.7656 0.93 1 19 0.59 +1Score > 33 indicates identity
Score > 30 indicates homology
KLSVAIAFVGDAK
2285 501.9205 1502.7397 1502.7148 16.6 0 19 0.29 +1Score > 41 indicates identity
Score > 26 indicates homology
MTQVGQTHTSGSIR + Deamidated (NQ)
340 314.6750 627.3354 627.3340 2.28 0 19 0.61 +1Score > 37 indicates identity
Score > 30 indicates homology
IGADPR
1082 483.7496 965.4846 965.4818 2.97 0 19 0.42 +1Score > 41 indicates identity
Score > 28 indicates homology
QYLSNNVK + Deamidated (NQ)
1637 304.4159 1213.6345 1213.6415 -5.74 1 19 0.59 +1Score > 40 indicates identity
Score > 30 indicates homology
LDNGQLRIQR + 2 Deamidated (NQ)
696 399.7593 797.5040 797.5123 -10.4 0 19 0.39 +1Score > 33 indicates identity
Score > 28 indicates homology
VLIGQLR
2872 889.9611 1777.9076 1777.9145 -3.85 0 19 0.27 +1Score > 40 indicates identity
Score > 26 indicates homology
GIIAHATPQCQINQVK + Deamidated (NQ)
272 586.3885 585.3812 585.3737 12.8 0 19 0.69 +1Score > 39 indicates identity
Score > 30 indicates homology
ILDIL
1066 480.7747 959.5348 959.5287 6.37 1 19 0.65 +1Score > 40 indicates identity
Score > 30 indicates homology
LIKTGNDAK + Deamidated (NQ)
1343 544.3105 1086.6064 1086.6073 -0.81 0 19 0.26 +1Score > 40 indicates identity
Score > 26 indicates homology
VPIQIQNFK + Deamidated (NQ)
1574 594.3202 1186.6258 1186.6081 15.0 1 19 0.81 +1Score > 41 indicates identity
Score > 31 indicates homology
KAQAAILDDLE + Deamidated (NQ)
496 351.7075 701.4004 701.3959 6.41 1 19 0.69 +1Score > 43 indicates identity
Score > 30 indicates homology
VQEVVK + Deamidated (NQ)
512 354.6889 707.3632 707.3602 4.27 0 19 0.81 +1Score > 41 indicates identity
Score > 31 indicates homology
FIADSR
2056 464.2610 1389.7612 1389.7616 -0.29 1 19 0.51 +1Score > 39 indicates identity
Score > 29 indicates homology
AQAKNLTFQQLK + Deamidated (NQ)
1276 527.8218 1053.6290 1053.6182 10.3 0 19 0.26 +1Score > 35 indicates identity
Score > 26 indicates homology
LNPATVLAVR + Deamidated (NQ)
1292 531.2968 1060.5790 1060.5665 11.8 0 19 0.39 +1Score > 41 indicates identity
Score > 28 indicates homology
LGANAVVNFR + Deamidated (NQ)
2596 545.6292 1633.8658 1633.8974 -19.3 1 19 0.9 +1Score > 39 indicates identity
Score > 31 indicates homology
YLQATPMVIGIRTR + Oxidation (M)
418 336.2270 670.4394 670.4377 2.54 0 19 0.62 +1Score > 37 indicates identity
Score > 30 indicates homology
VAGAILK
3380 1059.0403 2116.0660 2116.0973 -14.8 0 19 0.35 +1Score > 39 indicates identity
Score > 27 indicates homology
TVTVLNTILLQNMGAALTPE + 2 Deamidated (NQ); Oxidation (M)
986 465.2776 928.5406 928.5229 19.1 1 19 0.78 +1Score > 41 indicates identity
Score > 30 indicates homology
NLLEAQIK + Deamidated (NQ)
1483 385.2260 1152.6562 1152.6364 17.2 1 19 0.45 +1Score > 36 indicates identity
Score > 28 indicates homology
VQVVAPDRNR
440 675.4056 674.3983 674.3963 3.02 0 19 0.67 +1Score > 40 indicates identity
Score > 30 indicates homology
QVTISK
1024 316.5038 946.4896 946.4872 2.48 1 19 0.27 +1Score > 42 indicates identity
Score > 26 indicates homology
FGIDPDKR
770 421.7234 841.4322 841.4294 3.42 0 19 0.27 +1Score > 39 indicates identity
Score > 26 indicates homology
DPNDLLR
3221 1006.5229 2011.0312 2011.0473 -8.01 1 19 0.3 +1Score > 39 indicates identity
Score > 26 indicates homology
GQIVDTVVQEQRPSVLLE + 2 Deamidated (NQ)
3219 1006.4962 2010.9778 2010.9660 5.88 0 19 0.63 +1Score > 40 indicates identity
Score > 29 indicates homology
QLTHQQIYPHFADGSNR
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