MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita6 sp
MS data file : PRT1270_T-BRSC_6_20250714122635.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:38:56 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,869

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 301–400 (out of 3786)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
896 894.4700 893.4627 893.4607 2.31 1 23 0.7 +1Score > 41 indicates identity
Score > 34 indicates homology		 GISEGLYR
2105 471.2877 1410.8413 1410.8235 12.6 0 23 0.59 +1Score > 33 indicates identity LTPFINPLVLQR + Deamidated (NQ)
1425 565.8236 1129.6326 1129.6165 14.3 1 23 0.59 +1Score > 40 indicates identity
Score > 33 indicates homology		 KAMANILLQE
980 464.7127 927.4108 927.3974 14.5 1 23 0.29 +1Score > 38 indicates identity
Score > 30 indicates homology		 EFFDQNK + Deamidated (NQ)
500 352.2034 702.3922 702.4024 -14.5 0 23 0.96 +1Score > 44 indicates identity
Score > 35 indicates homology		 LGSASLR
2054 695.8868 1389.7590 1389.7616 -1.82 1 23 0.36 +1Score > 39 indicates identity
Score > 31 indicates homology		 AFIQQTIQKANK + Deamidated (NQ)
2957 610.3140 1827.9202 1827.9407 -11.2 1 23 0.66 +1Score > 40 indicates identity
Score > 33 indicates homology		 FKNIIASPLSAGFNYGK + 2 Deamidated (NQ)
873 440.7269 879.4392 879.4450 -6.55 1 22 0.33 +1Score > 42 indicates identity
Score > 30 indicates homology		 TEPYRSK
572 372.2196 742.4246 742.4225 2.91 0 22 0.33 +1Score > 41 indicates identity
Score > 30 indicates homology		 VATNLPK + Deamidated (NQ)
733 821.4821 820.4748 820.4728 2.44 0 22 0.83 +1Score > 37 indicates identity
Score > 34 indicates homology		 LTTMLVK + Oxidation (M)
1857 434.2315 1299.6727 1299.6531 15.1 1 22 0.71 +1Score > 41 indicates identity
Score > 33 indicates homology		 GNRSNGLITPNR + 2 Deamidated (NQ)
1794 425.5432 1273.6078 1273.6262 -14.5 1 22 0.86 +1Score > 41 indicates identity
Score > 34 indicates homology		 SRNDQVAVDIR + 2 Deamidated (NQ)
322 308.6750 615.3354 615.3452 -15.9 1 22 0.66 +1Score > 43 indicates identity
Score > 33 indicates homology		 AGNKAR
1476 574.7863 1147.5580 1147.5469 9.70 1 22 0.63 +1Score > 42 indicates identity
Score > 33 indicates homology		 GESSGNLQTVR + Deamidated (NQ)
1573 594.3198 1186.6250 1186.6346 -8.04 1 22 0.79 +1Score > 42 indicates identity
Score > 34 indicates homology		 AKASLIAQAWE
554 365.7342 729.4538 729.4497 5.70 1 22 0.85 +1Score > 36 indicates identity
Score > 34 indicates homology		 KISINR
1628 606.3563 1210.6980 1210.6921 4.88 0 22 0.99 +1Score > 36 indicates identity
Score > 35 indicates homology		 QVILQVGVINE
2211 490.9285 1469.7637 1469.7627 0.67 1 22 0.11 +1Score > 40 indicates identity
Score > 25 indicates homology		 HSVYGGVVPELASR
1900 660.8264 1319.6382 1319.6622 -18.2 1 22 0.42 +1Score > 41 indicates identity
Score > 31 indicates homology		 INQQAFQWKR + 2 Deamidated (NQ)
1140 497.2743 992.5340 992.5178 16.3 1 22 0.48 +1Score > 40 indicates identity
Score > 32 indicates homology		 SALLFDAEK
1041 317.8354 950.4844 950.4709 14.2 1 22 0.81 +1Score > 41 indicates identity
Score > 34 indicates homology		 AFEQGTLGK + Deamidated (NQ)
1203 339.5303 1015.5691 1015.5550 13.9 1 22 0.91 +1Score > 40 indicates identity
Score > 34 indicates homology		 SVGEAQVVVK + Deamidated (NQ)
934 456.7627 911.5108 911.5116 -0.87 1 22 0.52 +1Score > 38 indicates identity
Score > 32 indicates homology		 KIVDYFK
325 308.6770 615.3394 615.3452 -9.40 1 22 0.5 +1Score > 44 indicates identity
Score > 32 indicates homology		 AGNKAR
1952 672.8516 1343.6886 1343.6867 1.46 1 22 0.46 +1Score > 41 indicates identity
Score > 31 indicates homology		 AIKIMQNEPQR + Deamidated (NQ); Oxidation (M)
1368 553.3081 1104.6016 1104.5815 18.2 1 22 0.97 +1Score > 40 indicates identity
Score > 34 indicates homology		 QEIQFLQAK + Deamidated (NQ)
665 392.7699 783.5252 783.5218 4.39 0 22 0.46 +1Score > 32 indicates identity
Score > 31 indicates homology		 IIAQIVK
1512 581.8027 1161.5908 1161.5778 11.2 0 22 0.5 +1Score > 42 indicates identity
Score > 31 indicates homology		 QPAFTVQASGR + Deamidated (NQ)
1347 545.2843 1088.5540 1088.5349 17.6 0 22 0.94 +1Score > 42 indicates identity
Score > 34 indicates homology		 NASAATAAAVLE + Deamidated (NQ)
1479 575.7943 1149.5740 1149.5666 6.50 1 22 0.54 +1Score > 42 indicates identity
Score > 32 indicates homology		 AQIDFSAEIR + Deamidated (NQ)
490 351.7017 701.3888 701.3894 -0.82 0 22 0.74 +1Score > 42 indicates identity
Score > 33 indicates homology		 VSMLPR
2714 566.6347 1696.8823 1696.8858 -2.10 1 22 0.21 +1Score > 39 indicates identity
Score > 28 indicates homology		 FGALLIIDEVMTGFR + Oxidation (M)
1608 601.3365 1200.6584 1200.6437 12.3 0 22 0.43 +1Score > 41 indicates identity
Score > 31 indicates homology		 MLNVVWNAVR
1119 328.8549 983.5429 983.5512 -8.44 1 22 0.39 +1Score > 37 indicates identity
Score > 30 indicates homology		 QAKLNRPR + 2 Deamidated (NQ)
1551 589.2982 1176.5818 1176.5662 13.3 1 22 0.62 +1Score > 42 indicates identity
Score > 32 indicates homology		 AIAQAYDPEAK + Deamidated (NQ)
1613 301.4195 1201.6489 1201.6316 14.4 1 22 0.47 +1Score > 41 indicates identity
Score > 31 indicates homology		 WTSNALRQAR
1126 494.7856 987.5566 987.5726 -16.2 1 22 0.87 +1Score > 40 indicates identity
Score > 34 indicates homology		 LPHPAARAR
1538 586.8334 1171.6522 1171.6448 6.34 1 22 0.3 +1Score > 40 indicates identity
Score > 29 indicates homology		 NLSAAVLDELK
960 461.7375 921.4604 921.4590 1.62 0 22 0.95 +1Score > 42 indicates identity
Score > 34 indicates homology		 LMAVSGNSK + Oxidation (M)
872 440.7254 879.4362 879.4450 -9.96 1 22 0.5 +1Score > 42 indicates identity
Score > 31 indicates homology		 TEPYRSK
931 454.7468 907.4790 907.4651 15.4 1 22 0.87 +1Score > 41 indicates identity
Score > 34 indicates homology		 QINEYIK + Deamidated (NQ)
1586 398.2009 1191.5809 1191.5884 -6.30 0 22 0.84 +1Score > 41 indicates identity
Score > 33 indicates homology		 NPSVASQYGIR + Deamidated (NQ)
1378 554.7874 1107.5602 1107.5812 -18.9 0 22 0.56 +1Score > 42 indicates identity
Score > 32 indicates homology		 YNTLIDLQK + Deamidated (NQ)
1280 529.2871 1056.5596 1056.5563 3.15 1 22 0.48 +1Score > 40 indicates identity
Score > 31 indicates homology		 IALEQANAAR + Deamidated (NQ)
1755 627.2912 1252.5678 1252.5506 13.8 0 22 0.31 +1Score > 40 indicates identity
Score > 29 indicates homology		 NAMNGDAVAFSR + Deamidated (NQ)
1944 447.9218 1340.7436 1340.7664 -17.0 0 22 0.23 +1Score > 39 indicates identity
Score > 28 indicates homology		 VTVPDLSALAVTR
669 394.2078 786.4010 786.3984 3.38 1 22 0.81 +1Score > 42 indicates identity
Score > 33 indicates homology		 EANAIGGR
1517 583.7863 1165.5580 1165.5476 9.00 0 22 0.66 +1Score > 41 indicates identity
Score > 32 indicates homology		 NAPIHSQQNR + 2 Deamidated (NQ)
2337 507.2785 1518.8137 1518.8003 8.79 0 22 0.085 +1Score > 39 indicates identity
Score > 23 indicates homology		 MSVNINYLLLIPE + Deamidated (NQ)
2915 602.3056 1803.8950 1803.8811 7.67 1 22 0.096 +1Score > 40 indicates identity
Score > 24 indicates homology		 ANIVNLMLSSPEQIQK + 4 Deamidated (NQ); Oxidation (M)
612 381.2077 760.4008 760.3967 5.52 1 22 0.84 +1Score > 44 indicates identity
Score > 33 indicates homology		 ATQALEK + Deamidated (NQ)
1467 571.8627 1141.7108 1141.6958 13.2 1 21 0.45 +1Score > 30 indicates identity SLIIVNEILK + Deamidated (NQ)
844 435.2415 868.4684 868.4807 -14.1 0 21 0.55 +1Score > 38 indicates identity
Score > 31 indicates homology		 LPWPAGTK
2019 686.8860 1371.7574 1371.7544 2.24 0 21 0.82 +1Score > 39 indicates identity
Score > 33 indicates homology		 LQQNAMLLLATR + Deamidated (NQ)
1346 545.2833 1088.5520 1088.5648 -11.7 1 21 0.98 +1Score > 42 indicates identity
Score > 34 indicates homology		 DLMQQKAQK
3140 980.0209 1958.0272 1957.9938 17.1 0 21 0.1 +1Score > 39 indicates identity
Score > 24 indicates homology		 FLSQPFFVAAQFTGVDGK
2531 538.2913 1611.8521 1611.8580 -3.68 1 21 0.44 +1Score > 39 indicates identity
Score > 30 indicates homology		 LSARPGNEVNLLDSK
1990 682.3269 1362.6392 1362.6627 -17.2 1 21 0.59 +1Score > 41 indicates identity
Score > 31 indicates homology		 SSKADLQGSASPSK + Deamidated (NQ)
1057 319.8684 956.5834 956.5728 11.0 1 21 0.29 +1Score > 39 indicates identity
Score > 28 indicates homology		 KMLPNLLK + Deamidated (NQ)
1774 632.8172 1263.6198 1263.6129 5.52 0 21 0.49 +1Score > 41 indicates identity
Score > 31 indicates homology		 SDALASLMNGLR + Deamidated (NQ); Oxidation (M)
1002 470.7917 939.5688 939.5753 -6.87 0 21 0.51 +1Score > 36 indicates identity
Score > 31 indicates homology		 QPVLLITR + Deamidated (NQ)
1278 528.7946 1055.5746 1055.5723 2.20 1 21 0.28 +1Score > 39 indicates identity
Score > 28 indicates homology		 KLAAQPSDAR
1891 439.8836 1316.6290 1316.6096 14.7 0 21 0.67 +1Score > 41 indicates identity
Score > 32 indicates homology		 NATSDILVSPGNE + Deamidated (NQ)
698 400.7134 799.4122 799.4076 5.86 1 21 0.09 +1Score > 39 indicates identity
Score > 23 indicates homology		 KAGADPIE
1314 536.7875 1071.5604 1071.5560 4.14 1 21 0.96 +1Score > 42 indicates identity
Score > 33 indicates homology		 VQQALDELR + Deamidated (NQ)
1744 625.8325 1249.6504 1249.6740 -18.9 1 21 0.39 +1Score > 41 indicates identity
Score > 29 indicates homology		 VNELIYLVMR + Deamidated (NQ)
1529 585.3466 1168.6786 1168.6703 7.13 1 21 0.74 +1Score > 37 indicates identity
Score > 32 indicates homology		 GAEPETLLVIK
641 773.4170 772.4097 772.4191 -12.2 0 21 0.74 +1Score > 43 indicates identity
Score > 32 indicates homology		 QASALQR
2126 710.8428 1419.6710 1419.6738 -1.90 1 21 0.86 +1Score > 41 indicates identity
Score > 33 indicates homology		 MQETALLNMVPR + 2 Deamidated (NQ); Oxidation (M)
308 305.1724 608.3302 608.3282 3.39 0 21 0.26 +1Score > 38 indicates identity
Score > 28 indicates homology		 YASIR
571 371.7462 741.4778 741.4749 4.04 0 21 0.86 +1Score > 37 indicates identity
Score > 33 indicates homology		 LAQLIGK
1572 593.8245 1185.6344 1185.6353 -0.73 0 21 0.25 +1Score > 41 indicates identity
Score > 28 indicates homology		 QQLINLAATGR + 2 Deamidated (NQ)
2460 790.4490 1578.8834 1578.8729 6.66 1 21 0.52 +1Score > 36 indicates identity
Score > 31 indicates homology		 AAVQEGIVPGGGIALAR + Deamidated (NQ)
2722 567.9526 1700.8360 1700.8370 -0.58 0 21 0.082 +1Score > 40 indicates identity
Score > 23 indicates homology		 LGIDFQSDAQHVTIR + 2 Deamidated (NQ)
1743 625.8320 1249.6494 1249.6740 -19.7 1 21 0.89 +1Score > 41 indicates identity
Score > 33 indicates homology		 VNELIYLVMR + Deamidated (NQ)
392 329.7112 657.4078 657.4173 -14.4 0 21 0.52 +1Score > 41 indicates identity
Score > 31 indicates homology		 IVSLAR
1677 410.2276 1227.6610 1227.6823 -17.4 0 21 0.29 +1Score > 40 indicates identity
Score > 28 indicates homology		 NAVSTPLQTGLK
911 449.7428 897.4710 897.4668 4.70 0 21 0.49 +1Score > 39 indicates identity
Score > 30 indicates homology		 QQLTGHSK
3200 1001.0352 2000.0558 2000.0289 13.5 1 21 0.26 +1Score > 38 indicates identity
Score > 28 indicates homology		 VILTPTHLAIVMEYASGGE
404 334.7272 667.4398 667.4381 2.65 0 21 0.094 +1Score > 29 indicates identity
Score > 23 indicates homology		 VPLALR
479 350.7041 699.3936 699.4027 -13.0 0 21 0.94 +1Score > 39 indicates identity
Score > 33 indicates homology		 AIQAAAR
2217 369.2044 1472.7885 1472.8099 -14.6 1 21 0.7 +1Score > 40 indicates identity
Score > 32 indicates homology		 AVPQRVSLYNAVR + Deamidated (NQ)
1705 411.8955 1232.6647 1232.6652 -0.45 0 21 0.31 +1Score > 41 indicates identity
Score > 28 indicates homology		 TLNLLFALAQE + Deamidated (NQ)
1578 594.8350 1187.6554 1187.6510 3.77 1 21 0.68 +1Score > 40 indicates identity
Score > 32 indicates homology		 LTKSQLQQNK + Deamidated (NQ)
1222 343.1941 1026.5605 1026.5498 10.4 1 21 0.079 +1Score > 38 indicates identity
Score > 22 indicates homology		 RVYDTFVK
403 334.7089 667.4032 667.4017 2.29 0 21 0.11 +1Score > 30 indicates identity
Score > 24 indicates homology		 VPVPTR
2220 737.4052 1472.7958 1472.8086 -8.66 0 21 0.47 +1Score > 39 indicates identity
Score > 30 indicates homology		 SLDTASVDLSIKPK
1813 427.2411 1278.7015 1278.7197 -14.2 0 21 0.92 +1Score > 38 indicates identity
Score > 33 indicates homology		 WLLAHSVGQIR
375 322.7215 643.4284 643.4269 2.46 0 21 0.43 +1Score > 33 indicates identity
Score > 29 indicates homology		 LSVVVK
263 574.3348 573.3275 573.3235 7.10 0 21 0.69 +1Score > 42 indicates identity
Score > 32 indicates homology		 AGGTIR
1042 476.2503 950.4860 950.4709 16.0 1 21 0.65 +1Score > 41 indicates identity
Score > 31 indicates homology		 AQFENTLK + Deamidated (NQ)
1548 587.7801 1173.5456 1173.5553 -8.25 1 21 0.43 +1Score > 40 indicates identity
Score > 29 indicates homology		 IQGWENLLAE + 2 Deamidated (NQ)
1701 617.3152 1232.6158 1232.5997 13.1 0 21 0.68 +1Score > 42 indicates identity
Score > 31 indicates homology		 GQLTLTDQSNR + Deamidated (NQ)
1951 672.8496 1343.6846 1343.6867 -1.52 1 21 0.44 +1Score > 41 indicates identity
Score > 30 indicates homology		 AIKIMQNEPQR + Deamidated (NQ); Oxidation (M)
585 375.6934 749.3722 749.3742 -2.55 1 21 0.68 +1Score > 42 indicates identity
Score > 31 indicates homology		 MEVISR + Oxidation (M)
1284 529.7928 1057.5710 1057.5768 -5.39 1 21 0.68 +1Score > 42 indicates identity
Score > 31 indicates homology		 KIQTSAGQPK + Deamidated (NQ)
286 301.1878 600.3610 600.3595 2.56 0 21 0.33 +1Score > 44 indicates identity
Score > 28 indicates homology		 IGQVGK
749 415.7444 829.4742 829.4658 10.2 0 21 0.98 +1Score > 41 indicates identity
Score > 33 indicates homology		 QLTNVQK
2652 834.4499 1666.8852 1666.9076 -13.4 1 20 0.21 +1Score > 39 indicates identity
Score > 26 indicates homology		 LAMLQLTHNKLLQE + Oxidation (M)
2638 415.2335 1656.9049 1656.8868 10.9 1 20 0.37 +1Score > 38 indicates identity
Score > 29 indicates homology		 QIDMDILNNKALLR + Deamidated (NQ)
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