MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita6 sp
MS data file : PRT1270_T-BRSC_6_20250714122635.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:38:56 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,869

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3601–3700 (out of 3786)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4622 887.7342 3546.9077
4624 889.4818 3553.8981
4625 889.9823 3555.9001
4627 891.4671 3561.8393
4629 715.1613 3570.7701
4630 715.1718 3570.8226
4631 715.2015 3570.9711
4632 893.7540 3570.9869
4634 894.4956 3573.9533
4637 894.7455 3574.9529
4638 718.9875 3589.9011
4639 902.4794 3605.8885
4640 903.4821 3609.8993
4641 903.4862 3609.9157
4643 906.5004 3621.9725
4645 907.4638 3625.8261
4650 909.4579 3633.8025
4651 910.5317 3638.0977
4652 729.2104 3641.0156
4653 913.4753 3649.8721
4655 914.4688 3653.8461
4656 914.7241 3654.8673
4657 914.9766 3655.8773
4658 918.7621 3671.0193
4659 919.4653 3673.8321
4660 921.0032 3679.9837
4662 737.3753 3681.8401
4663 921.5123 3682.0201
4664 921.5125 3682.0209
4666 738.5475 3687.7011
4667 925.4720 3697.8589
4669 529.8569 3701.9474
4670 529.8569 3701.9474
4674 741.4211 3702.0691
4676 743.3848 3711.8876
4679 935.5112 3738.0157
4681 938.7704 3751.0525
4682 751.5907 3752.9171
4687 942.4915 3765.9369
4688 943.5111 3770.0153
4690 757.4047 3781.9871
4691 946.5092 3782.0077
4692 947.7726 3787.0613
4693 951.5007 3801.9737
4694 951.5161 3802.0353
4695 762.4254 3807.0906
4696 953.4396 3809.7293
4697 765.4325 3822.1261
4698 957.5161 3826.0353
4700 957.7608 3827.0141
4704 963.5265 3850.0769
4705 771.0231 3850.0791
4706 963.5288 3850.0861
4708 777.4031 3881.9791
4710 781.4130 3902.0286
4712 782.4266 3907.0966
4714 782.4283 3907.1051
4715 784.1778 3915.8526
4717 989.5434 3954.1445
4718 991.0458 3960.1541
4721 801.8307 4004.1171
4722 1002.0397 4004.1297
4723 1002.2609 4005.0145
4724 1002.2629 4005.0225
4725 1002.2858 4005.1141
4726 1002.2864 4005.1165
4727 1002.2864 4005.1165
4728 1002.3091 4005.2073
4729 803.3981 4011.9541
4730 1003.9972 4011.9597
4731 1003.9974 4011.9605
4732 803.4007 4011.9671
4733 803.6015 4012.9711
4734 804.4358 4017.1426
4735 1006.0232 4020.0637
4736 1006.0375 4020.1209
4737 1007.7559 4026.9945
4738 1008.0620 4028.2189
4739 1011.4965 4041.9569
4740 809.3996 4041.9616
4741 1013.2895 4049.1289
4742 1016.5164 4062.0365
4743 1016.5173 4062.0401
4744 1016.5357 4062.1137
4745 1016.5434 4062.1445
4746 1016.5443 4062.1481
4747 1016.5460 4062.1549
4748 813.4390 4062.1586
4749 1016.7787 4063.0857
4750 1017.0383 4064.1241
4751 1018.2514 4068.9765
4752 814.8052 4068.9896
4754 1018.5014 4069.9765
4755 1020.5305 4078.0929
4756 818.0455 4085.1911
4757 1022.3135 4085.2249
4759 1032.0624 4124.2205
4760 1032.0631 4124.2233
4761 1032.5321 4126.0993
4763 1035.7670 4139.0389
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