MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita6 sp
MS data file : PRT1270_T-BRSC_6_20250714122635.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:38:56 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,869

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3501–3600 (out of 3786)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4407 1033.5427 3097.6063
4415 1044.5585 3130.6537
4417 1046.2271 3135.6595
4424 785.4330 3137.7029
4425 1047.2289 3138.6649
4426 1050.2273 3147.6601
4427 787.9258 3147.6741
4428 787.9274 3147.6805
4429 1050.5571 3148.6495
4432 790.4242 3157.6677
4438 792.4265 3165.6769
4439 792.4279 3165.6825
4441 1056.5837 3166.7293
4442 1057.6187 3169.8343
4444 1057.9657 3170.8753
4447 636.5300 3177.6136
4454 1060.5826 3178.7260
4455 1061.5226 3181.5460
4459 1061.5261 3181.5565
4463 1064.9000 3191.6782
4467 800.1569 3196.5985
4468 800.4476 3197.7613
4470 801.7011 3202.7753
4471 1068.6014 3202.7824
4472 641.5639 3202.7831
4474 801.7053 3202.7921
4477 802.1709 3204.6545
4479 802.4218 3205.6581
4480 802.4225 3205.6609
4481 802.4254 3205.6725
4482 1070.2318 3207.6736
4484 1070.5587 3208.6543
4486 1070.5649 3208.6729
4490 1071.2211 3210.6415
4497 1078.1971 3231.5695
4498 808.9452 3231.7517
4499 809.1744 3232.6685
4500 1078.5890 3232.7452
4504 810.6482 3238.5637
4506 810.6997 3238.7697
4507 1080.6007 3238.7803
4509 1082.5431 3244.6075
4510 812.4467 3245.7577
4511 1082.9287 3245.7643
4512 813.4529 3249.7825
4513 813.4566 3249.7973
4514 1084.2745 3249.8017
4515 813.4586 3249.8053
4516 1084.9346 3251.7820
4517 651.9630 3254.7786
4520 1087.5525 3259.6357
4523 817.4613 3265.8161
4524 817.9099 3267.6105
4525 819.4162 3273.6357
4529 820.4237 3277.6657
4530 1094.5542 3280.6408
4532 1099.7841 3296.3305
4537 826.9551 3303.7913
4538 1102.5927 3304.7563
4539 661.9708 3304.8176
4541 827.7101 3306.8113
4544 1103.6030 3307.7872
4547 1103.6141 3307.8205
4548 827.9636 3307.8253
4550 828.9550 3311.7909
4551 1105.5653 3313.6741
4552 1105.9369 3314.7889
4554 1106.8970 3317.6692
4556 833.1854 3328.7125
4559 1112.2639 3333.7699
4562 668.3729 3336.8281
4566 836.2143 3340.8281
4567 836.4542 3341.7877
4568 669.5639 3342.7831
4573 840.4703 3357.8521
4574 1120.6178 3358.8316
4576 1120.9454 3359.8144
4578 673.1656 3360.7916
4579 841.2080 3360.8029
4580 841.9633 3363.8241
4582 1124.5920 3370.7542
4583 1125.2550 3372.7432
4584 844.4348 3373.7101
4585 678.7661 3388.7941
4591 681.9833 3404.8801
4593 683.5922 3412.9246
4595 1138.9377 3413.7913
4597 683.7928 3413.9276
4601 858.4440 3429.7469
4606 861.7344 3442.9085
4607 690.3768 3446.8476
4608 1163.5881 3487.7425
4609 874.7444 3494.9485
4610 1168.2646 3501.7720
4612 1173.3147 3516.9223
4613 706.1699 3525.8131
4615 883.9832 3531.9037
4616 1178.6373 3532.8901
4617 884.2320 3532.8989
4618 1178.6426 3532.9060
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