MASCOT Search Results

Search metadata
User : Jennifer
E-mail : [email protected]
Search title : Rita6 sp
MS data file : PRT1270_T-BRSC_6_20250714122635.mgf
Database : SwissProt 57.15 (515,203 sequences; 181,334,896 residues)
Timestamp : 12 Aug 2025 at 23:38:56 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : GluC_Trypsin
Fixed modifications : Carbamidomethyl (C)
Variable modifications : Deamidated (NQ), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.1 Da
Max missed cleavages : 1
Instrument type : ESI-FTICR
Number of queries : 4,869

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event. Individual ions scores > 39 indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Legend
Dupes Expect Rank U 1 2 Peptide
0.037 +2 GAYSLSLR significant
9 +1 GFFLFVEGGR top ranking
6.4e-005 +1 GSSIFGLAPGK significant and top ranking
1.3e-006 +1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-007 +1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-005 +1 U GSSIFGLAPGK unique
+2 5.7e-005 +1 LNTLETEEWFFK peptide has two duplicates
0.18 +1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (+) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (-) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Unassigned peptides, 3401–3500 (out of 3786)

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Peptide matches not assigned to protein families (no details means no match)

Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
Query Observed Mr(expt) Mr(calc) ppm  M Score Expect Rank Peptide
4118 910.8610 2729.5612
4119 683.3993 2729.5681
4120 910.8640 2729.5702
4121 683.5794 2730.2885
4126 913.5114 2737.5124
4127 916.4935 2746.4587
4132 917.8491 2750.5255
4133 917.8507 2750.5303
4134 917.8508 2750.5306
4135 689.1382 2752.5237
4140 690.3865 2757.5169
4141 920.1847 2757.5323
4144 693.6125 2770.4209
4145 924.5333 2770.5781
4146 555.2930 2771.4286
4151 925.8453 2774.5141
4152 925.8454 2774.5144
4155 926.5465 2776.6177
4166 930.5132 2788.5178
4174 933.8539 2798.5399
4176 936.5240 2806.5502
4177 936.8517 2807.5333
4187 937.1839 2808.5299
4193 704.8509 2815.3745
4195 939.5228 2815.5466
4199 705.8510 2819.3749
4200 941.1403 2820.3991
4206 944.5259 2830.5559
4210 948.5073 2842.5001
4213 948.8867 2843.6383
4219 952.8824 2855.6254
4223 716.8548 2863.3901
4225 955.8576 2864.5510
4226 956.1633 2865.4681
4229 956.4808 2866.4206
4230 478.9448 2867.6251
4231 574.5333 2867.6301
4234 574.7344 2868.6356
4235 718.8915 2871.5369
4236 958.5189 2872.5349
4237 958.5475 2872.6207
4238 958.5483 2872.6231
4240 959.4956 2875.4650
4247 721.1527 2880.5817
4250 722.4096 2885.6093
4254 963.5613 2887.6621
4256 724.1233 2892.4641
4265 967.5047 2899.4923
4269 726.1602 2900.6117
4270 726.1613 2900.6161
4271 968.1873 2901.5401
4272 968.5216 2902.5430
4275 582.5415 2907.6711
4276 970.4927 2908.4563
4277 728.1295 2908.4889
4278 970.5049 2908.4929
4279 728.1309 2908.4945
4286 585.3094 2921.5106
4287 975.5264 2923.5574
4288 585.9388 2924.6576
4292 732.9009 2927.5745
4294 978.2043 2931.5911
4298 979.1763 2934.5071
4302 984.5276 2950.5610
4312 988.7866 2963.3380
4313 741.8691 2963.4473
4314 742.3873 2965.5201
4317 990.5219 2968.5439
4319 990.8682 2969.5828
4320 991.2431 2970.7075
4323 746.4341 2981.7073
4324 995.5197 2983.5373
4329 997.5202 2989.5388
4331 748.4056 2989.5933
4336 998.5020 2992.4842
4338 750.6512 2998.5757
4340 1000.5352 2998.5838
4344 1006.2338 3015.6796
4348 756.0967 3020.3577
4353 1008.5144 3022.5214
4356 757.9297 3027.6897
4357 1010.5601 3028.6585
4359 1010.5667 3028.6783
4364 1013.5175 3037.5307
4365 760.3917 3037.5377
4366 760.6544 3038.5885
4369 763.4362 3049.7157
4373 1019.1684 3054.4834
4374 1019.2249 3054.6529
4379 1021.5336 3061.5790
4382 1023.4725 3067.3957
4383 767.8568 3067.3981
4385 1023.9034 3068.6884
4387 770.8905 3079.5329
4389 770.8926 3079.5413
4392 1028.2216 3081.6430
4393 771.4487 3081.7657
4399 1030.5085 3088.5037
4401 1031.5289 3091.5649
4403 773.9182 3091.6437
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